Recombinant Escherichia coli 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)

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Code CSB-EP332636ENV
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
dxr
Uniprot No.
Research Area
Cardiovascular
Alternative Names
dxr; ispC; yaeM; b0173; JW01681-deoxy-D-xylulose 5-phosphate reductoisomerase; DXP reductoisomerase; EC 1.1.1.267; 1-deoxyxylulose-5-phosphate reductoisomerase; 2-C-methyl-D-erythritol 4-phosphate synthase
Species
Escherichia coli (strain K12)
Source
E.coli
Expression Region
1-398aa
Target Protein Sequence
MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVEQCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIVGAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSLPQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKISVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTALNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKEVMRLAS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
48.4 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

This recombinant E.coli K12 dxr protein is an E.coli-expressed Full Length protein. At the theoretical level, the steps needed for obtaining a recombinant protein are pretty straightforward. Obtain the dxr gene, clone it in an expression vector, transform it into the host of choice, induce it, and then, the protein is ready for purification and characterization. In practice, however, dozens of things can go wrong. Poor growth of the host, inclusion body formation, protein inactivity, and even not obtaining any protein at all are some of the problems often found down the pipeline. So we choose the most suitable strain and vector for this recombinant dxr protein expression and adopt the most optimized expression condition and purification method. Finally, mild condition and subsequent cumbersome protein-refolding procedures ensure this dxr protein is soluble.

dxr (also known as ispC or yaeM) is a protein encoding gene that provides an instruction in making a protein named 1-deoxy-D-xylulose 5-phosphate reductoisomerase (short name is DXP reductoisomerase) in Escherichia coli. The protein encoded by this gene belongs to DXR family and has a role as an Escherichia coli metabolite. This enzyme converts 1-deoxy-D-xylulose 5-phosphate (DXP) into the branched compound 2-C-methyl-D-erythritol 4-phosphate (MEP). It has been shown to be the molecular target for fosmidomycin, a promising antimalarial drug.

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Target Background

Function
Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).
Gene References into Functions
  1. chemical mechanism of D-1-deoxyxylulose-5-phosphate reductoisomerase PMID: 17516600
Protein Families
DXR family
Database Links
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