Recombinant Caenorhabditis elegans Fem-3 mRNA-binding factor 1 (fbf-1), partial

Code CSB-YP885648CXY
MSDS
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Source Yeast
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Code CSB-EP885648CXY
MSDS
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Source E.coli
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Code CSB-EP885648CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885648CXY
MSDS
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Source Baculovirus
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Code CSB-MP885648CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
fbf-1
Uniprot No.
Alternative Names
fbf-1; H12I13.4Fem-3 mRNA-binding factor 1
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the control of stem cells and sex determination in the C.elegans hermaphrodite germline. May also play a role in the hermaphrodite germline proliferation and oogenesis. Binds specifically to the regulatory region of fem-3 3'-UTR and mediates the sperm/oocyte switch. Negatively regulates gld-3 expression, possibly by directly binding to two sites within the 3'-UTR of gld-3 isoform b. In association with the cye-1/cdk-2 complex, negatively regulates gld-1 expression in the distal germline cells of the mitotic zone. By binding to the 3'-UTR, represses phosphatase lip-1 expression in the distal part of the germline mitotic zone. Suppresses germline tumor formation by preventing the dedifferentiation of secondary spermatocytes.
Gene References into Functions
  1. It was concluded that FBF1 is a key functional transition fibre component that actively facilitates the ciliary entry of assembled IFT machinery. PMID: 24231678
  2. Data show that CPB-1 (cytoplasmic polyadenylation element binding protein homolog-1) alters the affinity of FBF (fem-3 mRNA binding factor) for specific RNA sequences. PMID: 23159558
  3. Data indicate that a 10-amino-acid region in germline development defective-3 (GLD-3) is required for fem-3 binding factor (FBF) binding, while a 7-amino-acid loop in FBF between PUF repeats 7 and 8 is necessary for GLD-3 binding. PMID: 23159559
  4. Here, the authors report that FBF/Pumilio, a conserved RNA-binding protein, promotes self-renewal of germline stem cells by repressing CKI-2(Cip/Kip), a Cyclin E/Cdk2 inhibitor. PMID: 21822213
  5. The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells. PMID: 20431119
  6. fbf-1 regulates the size of the mitotic region in helminth germ cells. PMID: 15525531
  7. FBF may coordinately repress sperm-specifying mRNAs to direct oogenesis and it represses the fog-1 mRNA to maintain FOG-1 at a low level appropriate for proliferation PMID: 16000383
  8. all fbf-1 puf-8 double mutants fail in the hermaphrodite sperm/oocyte switch PMID: 16037210
  9. A structural distortion in the central region of FBF imposes the requirement for an additional nucleotide to effect messenger RNA binding, thus defining its specificity. PMID: 16244662
  10. Fbf-1 redundantly inhibits primary vulval cell fate specification in two distinct pathways acting in the soma and in the germline PMID: 16908630
  11. Results show that the RNA-binding PUF protein FBF-1 is required in the adult olfactory sensory neurons of C. elegans for adaptation. PMID: 19146813

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Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed specifically in the germline (at protein level).
Database Links

KEGG: cel:CELE_H12I13.4

STRING: 6239.H12I13.4

UniGene: Cel.23295

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