Recombinant Human 2,4-dienoyl-CoA reductase, mitochondrial (DECR1)

Code CSB-YP624114HU
MSDS
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Source Yeast
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Code CSB-EP624114HU
MSDS
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Source E.coli
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Code CSB-EP624114HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP624114HU
MSDS
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Source Baculovirus
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Code CSB-MP624114HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DECR1
Uniprot No.
Alternative Names
1200012F07Rik; 2; 2,4 dienoyl CoA reductase [NADPH]; 2,4 dienoyl CoA reductase 1; 2,4 dienoyl CoA reductase mitochondrial; 2,4-dienoyl-CoA reductase; mitochondrial; 4 enoyl CoA reductase; 4-dienoyl-CoA reductase [NADPH]; 4-enoyl-CoA reductase [NADPH]; DECR 1; DECR; DECR_HUMAN; DECR1; Nadph; OTTMUSP00000004962; RP23-338J24.3; SDR18C1; Short chain dehydrogenase/reductase family 18C member 1
Species
Homo sapiens (Human)
Expression Region
35-335
Target Protein Sequence
NTEALQ SKFFSPLQKA MLPPNSFQGK VAFITGGGTG LGKGMTTLLS SLGAQCVIAS RKMDVLKATA EQISSQTGNK VHAIQCDVRD PDMVQNTVSE LIKVAGHPNI VINNAAGNFI SPTERLSPNA WKTITDIVLN GTAFVTLEIG KQLIKAQKGA AFLSITTIYA ETGSGFVVPS ASAKAGVEAM SKSLAAEWGK YGMRFNVIQP GPIKTKGAFS RLDPTGTFEK EMIGRIPCGR LGTVEELANL AAFLCSDYAS WINGAVIKFD GGEEVLISGE FNDLRKVTKE QWDTIEELIR KTKGS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in mitochondria. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.
Gene References into Functions
  1. Our study is the first to demonstrate the novel function of UA in NET formation and may provide insight into the management of patients with hyperuricemia. PMID: 24326071
  2. Structure and reactivity of human mitochondrial 2,4-dienoyl-CoA reductase PMID: 15531764
  3. Sequence alignment indicates that there are five highly conserved acidic residues, one of which (E276) might act as a proton donor. PMID: 15629123
  4. mutational defects cause chronic granulomatous disease in humans PMID: 18193341
  5. The luminal NADPH pool is an important antiapoptotic factor in neutrophil granulocytes. PMID: 18472006
  6. The TT genotype of C677T polymorphism in the MTHFR gene contributes to genetic susceptibility of acute ischemic stroke in a Singapore population. PMID: 19049547

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Involvement in disease
2,4-dienoyl-CoA reductase deficiency (DECRD)
Subcellular Location
Mitochondrion.
Protein Families
Short-chain dehydrogenases/reductases (SDR) family, 2,4-dienoyl-CoA reductase subfamily
Tissue Specificity
Heart = liver = pancreas > kidney >> skeletal muscle = lung.
Database Links

HGNC: 2753

OMIM: 222745

KEGG: hsa:1666

STRING: 9606.ENSP00000220764

UniGene: Hs.492212

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