Recombinant Human 28S ribosomal protein S29, mitochondrial (DAP3)

Code CSB-YP006497HU
MSDS
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Source Yeast
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Code CSB-EP006497HU
MSDS
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Source E.coli
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Code CSB-EP006497HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006497HU
MSDS
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Source Baculovirus
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Code CSB-MP006497HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DAP3
Uniprot No.
Alternative Names
28S ribosomal protein S29; 28S ribosomal protein S29 mitochondrial; bMRP10; DAP 3; DAP-3; Dap3; Death associated protein 3; Death-associated protein 3; DKFZp686G12159; FLJ12817; Ionizing radiation resistance conferring protein; MGC126058; MGC126059; Mitochondrial 28S ribosomal protein S29; mitochondrial; MITOCHONDRIAL RIBOSOMAL PROTEIN S29; MRP S29; MRP-S29; MRPS29; OTTHUMP00000033480; OTTHUMP00000033481; PSD-95/SAP90-binding protein 3; RT29_HUMAN; S29mt; SAP90/PSD-95-associated protein 3; SAPAP3
Species
Homo sapiens (Human)
Expression Region
19-398
Target Protein Sequence
GR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVMQ VKTFSEACLM VRKPALELLH YLKNTSFAYP AIRYLLYGEK GTGKTLSLCH VIHFCAKQDW LILHIPDAHL WVKNCRDLLQ SSYNKQRFDQ PLEASTWLKN FKTTNERFLN QIKVQEKYVW NKRESTEKGS PLGEVVEQGI TRVRNATDAV GIVLKELKRQ SSLGMFHLLV AVDGINALWG RTTLKREDKS PIAPEELALV HNLRKMMKND WHGGAIVSAL SQTGSLFKPR KAYLPQELLG KEGFDALDPF IPILVSNYNP KEFESCIQYY LENNWLQHEK APTEEGKKEL LFLSNANPSL LERHCAYL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in mediating interferon-gamma-induced cell death.
Gene References into Functions
  1. DAP3 plays important roles in mitochondrial function and dynamics PMID: 26306039
  2. DAP3 silencing contributes to breast carcinogenesis. PMID: 25738636
  3. Knockdown of DAP3 expression promoted cell migration. PMID: 24300973
  4. Study demonstrates an inverse association between DAP3 mRNA levels and tumour stage and clinical outcome in breast cancer. PMID: 22287761
  5. identified a novel DAP3-binding protein termed death ligand signal enhancer (DELE); results demonstrated the biological significance of DELE for apoptosis signal mediated by death receptors PMID: 20563667
  6. show that interferon-beta promoter stimulator 1 (IPS-1) binds DAP3 and induces anoikis by caspase activation. PMID: 19644511
  7. Functional interaction between the pro-apoptotic DAP3 and the glucocorticoid receptor PMID: 12099703
  8. not involved in TRAIL-induced apoptosis PMID: 12359235
  9. DAP3 has a role in the molecular pathway that culminates in fragmented mitochondria PMID: 15175341
  10. DAP3 gene might be associated with the mechanisms responsible for adult bronchial asthma and contribute to airway inflammation and remodeling PMID: 15179560
  11. DAP3 has a role in anoikis signaling and in apoptosis induction caused by cell detachment PMID: 15302871
  12. High DAP3 expression is correlated with thymoma stage PMID: 15679048
  13. study investigated the phosphorylation status of ribosomal DAP3 and mapped the phosphorylation sites by tandem mass spectrometry PMID: 18227431
  14. Complex I and DAP3, hNOA1 may play a role in mitochondrial respiration and apoptosis. PMID: 19103604
  15. DAP3 may participate in mitochondrial maintenance and play a role in the balance between mitochondrial homoeostasis and tumourigenesis in thyroid oncocytic tumors. PMID: 19536094

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Subcellular Location
Mitochondrion.
Protein Families
Mitochondrion-specific ribosomal protein mS29 family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 2673

OMIM: 602074

KEGG: hsa:7818

STRING: 9606.ENSP00000341692

UniGene: Hs.516746

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