Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

NUDT1

Uniprot No.

P36639

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

2-hydroxy-dATP diphosphatase; 7 8 dihydro 8 oxoguanine triphosphatase; 7; 8 oxo 7 8 dihydrodeoxyguanosine triphosphatase; 8 oxo 7 8 dihydroguanosine triphosphatase; 8 oxo dGTPase; 8-dihydro-8-oxoguanine triphosphatase; 8-oxo-dGTPase; 8ODP_HUMAN; MTH 1; MTH1; MutT human homolog 1; Nucleoside diphosphate linked moiety X motif 1; Nucleoside diphosphate linked moiety X type motif 1; Nucleoside diphosphate-linked moiety X motif 1; Nudix (nucleoside diphosphate linked moiety X) type motif 1; Nudix hydrolase 1; Nudix motif 1; Nudix type motif 1; NUDT 1; Nudt1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

19-197aa

Target Protein Sequence

MSGISPQQMGEPEGSWSGKNPGTMGASRLYTLVLVLQPQRVLLGMKKRGFGAGRWNGFGGKVQEGETIEDGARRELQEESGLTVDALHKVGQIVFEFVGEPELMDVHVFCTDSIQGTPVESDEMRPCWFQLDQIPFKDMWPDDSYWFPLLLQKKKFHGYFKFQGQDTILDYTLREVDTV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

20.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Human NUDT1 recombinant protein was produced in E.coli, where the gene sequence encoding Human NUDT1 (19-197aa) was expressed with Tag-Free. The purity of this NUDT1 protein was greater than 85% by SDS-PAGE.
NUDT1's primary function is to prevent the accumulation of 7,8-dihydro-8-oxoguanine triphosphate (8-oxo-dGTP) in cells. This compound is one of the most common oxidative damages to DNA and can lead to DNA mutations and genomic instability, increasing the risk of cancer and other diseases. NUDT1 hydrolyzes 8-oxo-dGTP, converting it into 8-oxo-dGMP, thereby preventing this oxidative damage from being incorporated into newly synthesized DNA strands. This helps maintain the integrity and stability of DNA. NUDT1 has generated significant interest in cancer research. Some studies have found that the activity of NUDT1 is reduced in certain cancer cells, leading to the accumulation of oxidative damage in DNA, which promotes the development of tumors. Therefore, researchers are investigating whether defects in NUDT1 can be exploited to develop new anti-cancer drugs or treatment approaches.

Target Background

Function

Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A. Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA. Through this antimutagenic activity protects cells from oxidative stress.

Gene References into Functions

overall our cell viability data indicates that targeting MTH1 will likely not be an across-the-board effective non-small cell lung cancer therapeutic strategy; rather it induces non-cytotoxic DNA damage that could promote cancer heterogeneity and evolution. PMID: 29661172
MTH1 is required for maintaining migration and invasion potential of human thyroid cancer cells. PMID: 30055508
PRDX1 and MTH1 cooperate to prevent accumulation of oxidized guanine in the genome PMID: 29773556
Results provide evidence that MTH1 is not essential for cancer cell survival. PMID: 27210421
CDT1, MCM7, and NUDT1 were shown to be up-regulated in hepatocellular carcinoma tissues and provide a more accurate diagnosis than alpha-fetal protein alone. PMID: 29442275
Our results reveal a novel antitumor mechanism of (S)-crizotinib in NSCLC which involves activation of ROS-dependent ER stress apoptotic pathway and is independent of MTH1 inhibition PMID: 28882182
this study showed that MTH1 protein expression was closely related to factors associated with a high malignant potential and poor patient survival PMID: 28577950
method for predicting individual residue contributions to enzyme specificity and binding-site energies, and its application to MTH1. PMID: 27714533
Data indicate the specificity of the enzyme 8-oxo-dGTPase MTH1 toward the substrate 8-oxo-dGTP. PMID: 27350386
We demonstrate that in order to kill cancer cells MTH1 inhibitors must also introduce oxidized nucleotides into DNA. Furthermore, we describe TH1579 as a best-in-class MTH1 inhibitor, which we expect to be useful in order to further validate the MTH1 inhibitor concept. PMID: 27827301
Data indicate a positive correlation of Skp2 and MTH1 expression in melanoma cell lines and patient specimens. PMID: 28947420
Results show that MTH1 is overexpressed in esophageal squamous cell carcinoma, and suggestive of disease progression. PMID: 27917618
MTH1 together with MYH plays an important role in protection against mutations induced by modified dNTPs during chronic oxidative stress. PMID: 28340109
Reduced MUTYH, MTH1, and OGG1 expression and TP53 mutations occur in diffuse-type adenocarcinoma of gastric cardia. PMID: 26980051
Analyses of bond lengths with high-resolution X-ray data and the relationship between the structure and enzymatic activity revealed that hMTH1 recognizes the different oxidized nucleotides via an exchange of the protonation state at two neighboring aspartate residues (Asp-119 and Asp-120) in its substrate binding pocket PMID: 28035004
MTH1 inhibition may offer a general approach to treat cancers characterized by deregulated hypoxia signaling or redox imbalance PMID: 26862114
MTH-1 expression in colorectal cancer cells was upregulated via HIF-1alpha in response to hypoxic stress, emphasizing the crucial role of HIF-1alpha-induced MTH-1 in tumor growth. PMID: 26730155
activity of MTH1 in different breast cancer cell lines has been detected, implying the potential application of this assay method for biomedical research and clinical diagnose in the future PMID: 26755138
a novel approach involving liquid chromatography-isotope-dilution tandem mass spectrometry to positively identify and accurately quantify MTH1 in human tissues, is reported. PMID: 26202347
MTH1 is the most prominent sanitizer of the cellular dNTP pool known to date. PMID: 26238318
MTH1 expression is required for effective transformation of epithelial cells by oncogenic HRAS. PMID: 25893378
Results show that MTH1 plays no role in protecting the cells against ultraviolet radiation-induced cytogenetic damage. PMID: 26520386
Results suggest that the hMTH1-mediated maintenance of mtDNA stability protects cells from the susceptibility to oxidant injury associated with polyQ-expanded Htt, defends against 3-nitropropionic acid-induced neurodegeneration PMID: 22974734
results indicate MTH1 is a novel and critical component of oncogenic KRAS-associated malignancy and its inhibition is likely to yield significant tumor-suppressive outcomes in KRAS-driven tumors. PMID: 25023700
The ectopic expression of hMTH1 in the chloroplasts and mitochondria of Arabidopsis enhanced oxidative stress tolerance by activating the poly(ADP-ribosyl)ation (PAR)reaction and suppressing programmed cell death. PMID: 24928220
Data suggest that hOGG1 could compensate for hMTH1 during oxidative DNA damage caused by H2O2, whereas hMTH1 could not compensate sufficiently for hOGG1 during the process. PMID: 25127756
cancer cells require MTH1 activity to avoid incorporation of oxidized dNTPs, resulting in DNA damage and cell death; validation of MTH1 as an anticancer target in vivo PMID: 24695224
MTH1 protects cells from mutagenesis induced by ultraviolet ray A and B, but not ultraviolet ray C.hMTH1 prevents induction of transition-type mutations at AT and GC post ultraviolet ray A irradiation. PMID: 24144844
X-ray crystallographic analysis of MTH1 protein structure PMID: 23295485
The risk of type 2 diabetes in the Chinese population is increased from the combined effects of AluYb8MUTYH with either hMTH1 c.247G>A or variants in the 5\'-UTR of the hOGG1. PMID: 23396182
The study provides insight into the influence of MTH1 levels on the epithelial-mesenchymal transition phenotype and Akt activation in RAS-transformed HMLE breast epithelial cells. PMID: 22790201
MutT homolog-1 attenuates oxidative DNA damage and delays photoreceptor cell death in inherited retinal degeneration. PMID: 22841817
These results suggest that MTH1 deficiency might be a causative factor for aging and age-related disorders. PMID: 21538080
results indicate that the nucleotide pool is a significant target for UVA-induced mutations and implicates that hMTH1 plays an important role in protecting cells from UVA-induced oxidative stress PMID: 21784087
the structures of human MTH1 (1.9A) and its complex with the product 8-oxo-dGMP PMID: 21787772
Manipulation of miR-145 expression modulates epidermal growth factor receptor (EGFR) and NUDT1 mRNA expressions. PMID: 21289483
The expression levels of hMTH1 mRNA are highly correlated with hepatic levels of 8-oxo-dG and tail moment, suggesting that hMTH1 gene expression represents a molecular marker of oxidative DNA damage. PMID: 21421019
Two rare variants (OGG1 c.137G>A; MUTYH c.1187G>A) and one common polymorphism (NUDT1 c.426C>T) were associated with colorectal cancer risk. PMID: 21355073
human MTH1, MTH2, and NUDT5 proteins act as a defense against the mutagenesis induced by oxidized dGTP. PMID: 20144704
Trp-117 is essential for MTH1 to recognize both 8-oxo-dGTP and 2-hydroxy-dATP, whereas Asp-119 is only essential for recognizing 2-hydroxy-dATP, thus suggesting that origins of the substrate-binding pockets for MTH1 and MutT are different PMID: 11756418
8-Chloro-dGTP, a hypochlorous acid-modified nucleotide, is hydrolyzed by hMTH1, the human MutT homolog. PMID: 11852070
Role of tryptophan residues in the recognition of mutagenic oxidized nucleotides by human antimutator MTH1 protein PMID: 12051941
These results suggested that increased expression of hMTH in peripheral lymphocytes may be a risk factor for prostate cancer and support our priori hypothesis. PMID: 12619034
cleaves 8-oxo-dGTP to 8-oxo-GMP, an unusable form for DNA synthesis PMID: 12717453
Elevated levels of hMTH1 protein is asociated with non-small-cell lung carcinomas PMID: 12757855
MTH1 protects cells from H2O2-induced cell dysfunction and death by hydrolyzing oxidized purine nucleotides including 8-oxo-dGTP and 2-OH-dATP. PMID: 12857738
dGDP and dADP, at physiological concentrations not exceeding 5 microM and GDP at mean concentration of 30 microM, taken together, can decrease the cellular hMTH1 enzymatic activity vs. 8-oxo-dGTP PMID: 12957652
These results clarify the effects of the anti/syn conformation and the functional groups on the 2 and 6 positions of the purine ring on the recognition by the human MTH1 protein. PMID: 15095864
study presents the solution structure of Nudix family hydrolase MTH1 solved by multidimensional heteronuclear NMR spectroscopy PMID: 15133035
the Met allele at codon 83 of MTH1 gene might be involved in the development of type 1 diabetes mellitus in the Japanese female population. PMID: 15516784

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Subcellular Location

[Isoform p18]: Cytoplasm, cytosol. Mitochondrion matrix. Nucleus.; [Isoform p26]: Mitochondrion matrix.

Protein Families

Nudix hydrolase family

Tissue Specificity

Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.

Database Links

HGNC: 8048

OMIM: 600312

KEGG: hsa:4521

STRING: 9606.ENSP00000339503

UniGene: Hs.534331

Code	CSB-EP016154HU
Abbreviation	Recombinant Human NUDT1 protein
MSDS	MSDS Datasheet
Size	US$422
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human 7,8-dihydro-8-oxoguanine triphosphatase (NUDT1)

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