Product Details

Uniprot No.

Target Names

NUDT1

Alternative Names

2-hydroxy-dATP diphosphatase antibody; 7 8 dihydro 8 oxoguanine triphosphatase antibody; 7 antibody; 8 oxo 7 8 dihydrodeoxyguanosine triphosphatase antibody; 8 oxo 7 8 dihydroguanosine triphosphatase antibody; 8 oxo dGTPase antibody; 8-dihydro-8-oxoguanine triphosphatase antibody; 8-oxo-dGTPase antibody; 8ODP_HUMAN antibody; MTH 1 antibody; MTH1 antibody; MutT human homolog 1 antibody; Nucleoside diphosphate linked moiety X motif 1 antibody; Nucleoside diphosphate linked moiety X type motif 1 antibody; Nucleoside diphosphate-linked moiety X motif 1 antibody; Nudix (nucleoside diphosphate linked moiety X) type motif 1 antibody; Nudix hydrolase 1 antibody; Nudix motif 1 antibody; Nudix type motif 1 antibody; NUDT 1 antibody; Nudt1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human NUDT1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8B10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NUDT1, also known as MTH1, serves as a critical guardian of genomic integrity by hydrolyzing oxidized nucleotide triphosphates such as 8-oxo-dGTP, preventing their misincorporation into DNA during replication. This sanitization function has garnered significant research attention, particularly in cancer biology, where NUDT1 overexpression in tumor cells may represent an adaptive response to elevated oxidative stress. Understanding NUDT1 expression and function provides valuable insights into DNA damage response pathways and potential therapeutic vulnerabilities in malignant cells.

This recombinant monoclonal antibody, clone 8B10, offers researchers the reproducibility essential for longitudinal studies and cross-laboratory comparisons. Generated from a defined sequence against a synthetic peptide derived from human NUDT1, the antibody delivers consistent performance across experiments, eliminating the lot-to-lot variability that can complicate data interpretation with traditional hybridoma-derived reagents.

Validation studies demonstrate robust detection of NUDT1 across a diverse panel of human cell lines in Western blot applications, including MCF-7, A549, Jurkat, SH-SY5Y, HeLa, HEK293, SW620, and K562 lysates. The antibody produces a clean band at the predicted molecular weight of 18 kDa, confirming specific target recognition. Flow cytometry validation in Jurkat cells further establishes utility for single-cell analysis of intracellular NUDT1 levels, with clear separation between specific staining and isotype control populations.

The antibody's compatibility with Western blotting, flow cytometry, and ELISA provides workflow flexibility for researchers investigating oxidative stress responses, DNA repair mechanisms, and cancer cell metabolism. Whether profiling NUDT1 expression across tumor models or examining its regulation under genotoxic conditions, this reagent supports rigorous, reproducible experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A. Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA. Through this antimutagenic activity protects cells from oxidative stress.

Gene References into Functions

overall our cell viability data indicates that targeting MTH1 will likely not be an across-the-board effective non-small cell lung cancer therapeutic strategy; rather it induces non-cytotoxic DNA damage that could promote cancer heterogeneity and evolution. PMID: 29661172
MTH1 is required for maintaining migration and invasion potential of human thyroid cancer cells. PMID: 30055508
PRDX1 and MTH1 cooperate to prevent accumulation of oxidized guanine in the genome PMID: 29773556
Results provide evidence that MTH1 is not essential for cancer cell survival. PMID: 27210421
CDT1, MCM7, and NUDT1 were shown to be up-regulated in hepatocellular carcinoma tissues and provide a more accurate diagnosis than alpha-fetal protein alone. PMID: 29442275
Our results reveal a novel antitumor mechanism of (S)-crizotinib in NSCLC which involves activation of ROS-dependent ER stress apoptotic pathway and is independent of MTH1 inhibition PMID: 28882182
this study showed that MTH1 protein expression was closely related to factors associated with a high malignant potential and poor patient survival PMID: 28577950
method for predicting individual residue contributions to enzyme specificity and binding-site energies, and its application to MTH1. PMID: 27714533
Data indicate the specificity of the enzyme 8-oxo-dGTPase MTH1 toward the substrate 8-oxo-dGTP. PMID: 27350386
We demonstrate that in order to kill cancer cells MTH1 inhibitors must also introduce oxidized nucleotides into DNA. Furthermore, we describe TH1579 as a best-in-class MTH1 inhibitor, which we expect to be useful in order to further validate the MTH1 inhibitor concept. PMID: 27827301
Data indicate a positive correlation of Skp2 and MTH1 expression in melanoma cell lines and patient specimens. PMID: 28947420
Results show that MTH1 is overexpressed in esophageal squamous cell carcinoma, and suggestive of disease progression. PMID: 27917618
MTH1 together with MYH plays an important role in protection against mutations induced by modified dNTPs during chronic oxidative stress. PMID: 28340109
Reduced MUTYH, MTH1, and OGG1 expression and TP53 mutations occur in diffuse-type adenocarcinoma of gastric cardia. PMID: 26980051
Analyses of bond lengths with high-resolution X-ray data and the relationship between the structure and enzymatic activity revealed that hMTH1 recognizes the different oxidized nucleotides via an exchange of the protonation state at two neighboring aspartate residues (Asp-119 and Asp-120) in its substrate binding pocket PMID: 28035004
MTH1 inhibition may offer a general approach to treat cancers characterized by deregulated hypoxia signaling or redox imbalance PMID: 26862114
MTH-1 expression in colorectal cancer cells was upregulated via HIF-1alpha in response to hypoxic stress, emphasizing the crucial role of HIF-1alpha-induced MTH-1 in tumor growth. PMID: 26730155
activity of MTH1 in different breast cancer cell lines has been detected, implying the potential application of this assay method for biomedical research and clinical diagnose in the future PMID: 26755138
a novel approach involving liquid chromatography-isotope-dilution tandem mass spectrometry to positively identify and accurately quantify MTH1 in human tissues, is reported. PMID: 26202347
MTH1 is the most prominent sanitizer of the cellular dNTP pool known to date. PMID: 26238318
MTH1 expression is required for effective transformation of epithelial cells by oncogenic HRAS. PMID: 25893378
Results show that MTH1 plays no role in protecting the cells against ultraviolet radiation-induced cytogenetic damage. PMID: 26520386
Results suggest that the hMTH1-mediated maintenance of mtDNA stability protects cells from the susceptibility to oxidant injury associated with polyQ-expanded Htt, defends against 3-nitropropionic acid-induced neurodegeneration PMID: 22974734
results indicate MTH1 is a novel and critical component of oncogenic KRAS-associated malignancy and its inhibition is likely to yield significant tumor-suppressive outcomes in KRAS-driven tumors. PMID: 25023700
The ectopic expression of hMTH1 in the chloroplasts and mitochondria of Arabidopsis enhanced oxidative stress tolerance by activating the poly(ADP-ribosyl)ation (PAR)reaction and suppressing programmed cell death. PMID: 24928220
Data suggest that hOGG1 could compensate for hMTH1 during oxidative DNA damage caused by H2O2, whereas hMTH1 could not compensate sufficiently for hOGG1 during the process. PMID: 25127756
cancer cells require MTH1 activity to avoid incorporation of oxidized dNTPs, resulting in DNA damage and cell death; validation of MTH1 as an anticancer target in vivo PMID: 24695224
MTH1 protects cells from mutagenesis induced by ultraviolet ray A and B, but not ultraviolet ray C.hMTH1 prevents induction of transition-type mutations at AT and GC post ultraviolet ray A irradiation. PMID: 24144844
X-ray crystallographic analysis of MTH1 protein structure PMID: 23295485
The risk of type 2 diabetes in the Chinese population is increased from the combined effects of AluYb8MUTYH with either hMTH1 c.247G>A or variants in the 5\'-UTR of the hOGG1. PMID: 23396182
The study provides insight into the influence of MTH1 levels on the epithelial-mesenchymal transition phenotype and Akt activation in RAS-transformed HMLE breast epithelial cells. PMID: 22790201
MutT homolog-1 attenuates oxidative DNA damage and delays photoreceptor cell death in inherited retinal degeneration. PMID: 22841817
These results suggest that MTH1 deficiency might be a causative factor for aging and age-related disorders. PMID: 21538080
results indicate that the nucleotide pool is a significant target for UVA-induced mutations and implicates that hMTH1 plays an important role in protecting cells from UVA-induced oxidative stress PMID: 21784087
the structures of human MTH1 (1.9A) and its complex with the product 8-oxo-dGMP PMID: 21787772
Manipulation of miR-145 expression modulates epidermal growth factor receptor (EGFR) and NUDT1 mRNA expressions. PMID: 21289483
The expression levels of hMTH1 mRNA are highly correlated with hepatic levels of 8-oxo-dG and tail moment, suggesting that hMTH1 gene expression represents a molecular marker of oxidative DNA damage. PMID: 21421019
Two rare variants (OGG1 c.137G>A; MUTYH c.1187G>A) and one common polymorphism (NUDT1 c.426C>T) were associated with colorectal cancer risk. PMID: 21355073
human MTH1, MTH2, and NUDT5 proteins act as a defense against the mutagenesis induced by oxidized dGTP. PMID: 20144704
Trp-117 is essential for MTH1 to recognize both 8-oxo-dGTP and 2-hydroxy-dATP, whereas Asp-119 is only essential for recognizing 2-hydroxy-dATP, thus suggesting that origins of the substrate-binding pockets for MTH1 and MutT are different PMID: 11756418
8-Chloro-dGTP, a hypochlorous acid-modified nucleotide, is hydrolyzed by hMTH1, the human MutT homolog. PMID: 11852070
Role of tryptophan residues in the recognition of mutagenic oxidized nucleotides by human antimutator MTH1 protein PMID: 12051941
These results suggested that increased expression of hMTH in peripheral lymphocytes may be a risk factor for prostate cancer and support our priori hypothesis. PMID: 12619034
cleaves 8-oxo-dGTP to 8-oxo-GMP, an unusable form for DNA synthesis PMID: 12717453
Elevated levels of hMTH1 protein is asociated with non-small-cell lung carcinomas PMID: 12757855
MTH1 protects cells from H2O2-induced cell dysfunction and death by hydrolyzing oxidized purine nucleotides including 8-oxo-dGTP and 2-OH-dATP. PMID: 12857738
dGDP and dADP, at physiological concentrations not exceeding 5 microM and GDP at mean concentration of 30 microM, taken together, can decrease the cellular hMTH1 enzymatic activity vs. 8-oxo-dGTP PMID: 12957652
These results clarify the effects of the anti/syn conformation and the functional groups on the 2 and 6 positions of the purine ring on the recognition by the human MTH1 protein. PMID: 15095864
study presents the solution structure of Nudix family hydrolase MTH1 solved by multidimensional heteronuclear NMR spectroscopy PMID: 15133035
the Met allele at codon 83 of MTH1 gene might be involved in the development of type 1 diabetes mellitus in the Japanese female population. PMID: 15516784

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Subcellular Location

[Isoform p18]: Cytoplasm, cytosol. Mitochondrion matrix. Nucleus.; [Isoform p26]: Mitochondrion matrix.

Protein Families

Nudix hydrolase family

Tissue Specificity

Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.

Database Links

HGNC: 8048

OMIM: 600312

KEGG: hsa:4521

STRING: 9606.ENSP00000339503

UniGene: Hs.534331

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Code	CSB-RA037177A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate(30µg), A549 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), HeLa whole cell lysate(30µg), HEK293 whole cell lysate(30µg), SW620 whole cell lysate(30µg), K562 whole cell lysate(30µg) All lanes: NUDT1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3min Overlay Peak curve showing jurkat cells stained with CSB-RA037177A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NUDT1 Recombinant Monoclonal Antibody

Product Details

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Target Background