Recombinant Human A-kinase anchor protein 1, mitochondrial (AKAP1), partial

Code CSB-YP835689HU
MSDS
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Source Yeast
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Code CSB-EP835689HU
MSDS
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Source E.coli
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Code CSB-EP835689HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835689HU
MSDS
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Source Baculovirus
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Code CSB-MP835689HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AKAP1
Uniprot No.
Alternative Names
A kinase anchor protein; A-kinase anchor protein 1; A-kinase anchor protein 149 kDa; AKAP 149; AKAP; Akap1; AKAP1_HUMAN; D-AKAP-1; DAKAP1; Dual specificity A-kinase-anchoring protein 1; mitochondrial; PRKA1; Protein kinase A-anchoring protein 1; Protein kinase A1; S-AKAP84; SAKAP84; Spermatid A-kinase anchor protein 84
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. Involved in mitochondrial-mediated antiviral innate immunity. Promotes translocation of NDUFS1 into mitochondria to regulate mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) activity.
Gene References into Functions
  1. AKAP1 is a transcriptional target of Myc, and it supports mTOR pathway and the growth of cancer cells. PMID: 28569781
  2. Furthermore, we discuss the role of hypoxia in prompting cellular stress and damage, which has been demonstrated to mediate the proteosomal degradation of AKAP121, leading to an increase in reactive oxgyen species production, mitochondrial dysfunction, and ultimately cell death. [review] PMID: 26825124
  3. Functional characterization revealed an undescribed role for AMPK-dependent phosphorylation of AKAP1 in mitochondrial respiration. PMID: 26437602
  4. AKAP 149-PKA-PDE4A complex localization is related with YTX effect in K-562 cell line PMID: 24813785
  5. In the case of the beta2AR, this process is facilitated by the presence of A-Kinase Anchoring Proteins (AKAPs) that serve as scaffolding proteins for the L-type calcium channel and the beta2AR complex. PMID: 23557075
  6. AKAP1 anchors Star mRNA at the mitochondria, thus stabilizing the translational complex at this organelle, a situation that might affect STAR production and steroidogenesis. PMID: 23077346
  7. In tumoural lymphocytes, yessotoxin decreases AKAP149 cytosolic expression and increases cAMP levels which leads to cell death. PMID: 22807343
  8. Shp2 is a component of the AKAP-Lbc complex and is inhibited by protein kinase A under pathological hypertrophic conditions in the heart. PMID: 23045525
  9. AMY-1 interacts with this and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits cAMP-dependent protein kinase activity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex. PMID: 12414807
  10. RIalpha and RIbeta homodimers as well as an RIalpha:RIbeta heterodimer and several of the mutants were able to bind to the R-binding domain of AKAP149/D-AKAP1 PMID: 12634056
  11. AKAP149-regulated PP1 activity at the NE during G1 is required to maintain nuclear integrity and cell survival. PMID: 12697839
  12. DAKAP1alpha overexpression induced regulatory (R) or catalytic (C) subunits of PKA to outer mitochondrial colocalization and showed similar regulation PMID: 17884635
  13. both the constitutive and cAMP-induced release of TNFR1 exosome-like vesicles occur via PKA-dependent pathways that are regulated by the anchoring of RIIbeta to BIG2 via AKAP domains B and C PMID: 18625701
  14. Results suggest that the interaction between reverse transcriptase and AKAP149 in infected cells may play an important role in HIV-1 reverse transcription. PMID: 18786546
  15. Protein phosphatase 1 binding occurs through a conserved RVXF motif found in the KH domain of AKAP149. PMID: 19074462

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Subcellular Location
Mitochondrion outer membrane. Mitochondrion.
Tissue Specificity
Isoform 1 is detected in thymus, prostate, testis, ovary, colon and small intestine. Isoform 2 is highly expressed in testis and detected at much lower levels in kidney, pancreas, liver, lung and brain.
Database Links

HGNC: 367

OMIM: 602449

KEGG: hsa:8165

STRING: 9606.ENSP00000337736

UniGene: Hs.463506

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