Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ACTA1

Uniprot No.

P68133

Research Area

Signal Transduction

Alternative Names

a actin; ACTA; ACTA1; ACTA2; ACTC; ACTC1; Actin; ACTS_HUMAN; ACTSA; Alpha 2 actin; alpha skeletal muscle; Alpha-actin-1; Cardiac muscle alpha actin 1; Skeletal muscle alpha actin 1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

3-377aa

Target Protein Sequence

DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

45.8kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human ACTA1 covers amino acids 3-377. This ACTA1 protein is expected to have a theoretical molecular weight of 45.8 kDa. Expression of this ACTA1 protein is conducted in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of ACTA1, making it easier to detect and purify the ACTA1 recombinant protein in the later stages of expression and purification.

Actin, alpha skeletal muscle (ACTA1) is a widely expressed protein in muscle tissue, primarily involved in the study of muscle biology. In this field, ACTA1 plays a crucial role as it constitutes an essential component of the cell skeleton in muscle cells. Researchers focus on understanding the function of ACTA1 in muscle contraction and movement processes, as well as its role in maintaining muscle structure and stability. One of the most significant research directions is exploring the relationship between ACTA1 and muscle diseases, such as muscular dystrophy. Scientists also investigate the mechanisms of ACTA1 involvement in muscle regeneration and repair, which holds important implications for treating muscle injuries and diseases.

Target Background

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Gene References into Functions

Our study demonstrates that high ActA level is an independent prognosis factor of survival in cancer patients. More than a basic marker of the severity of the neoplastic disease or of the inflammatory process, ActA seems to influence survival by contributing to the development of cachexia and loss of skeletal muscle mass. PMID: 28712119
The study confirmed ACTA1 mutations in four patients, including one with intranuclear rods, one with large intracytoplasmic aggregates, and two with nemaline intracytoplasmic rods. PMID: 28780987
Study shows that clinically severe ACTA1-related myopathy can present with muscle morphological findings suggestive of cytoplasmic body myopathy in the absence of definite nemaline rods. PMID: 28416349
Shorter than normal thin filament length contributes to the impaired force generation in patients with thin filament myopathy, but only in those who harbor specific mutations in NEB or ACTA1. PMID: 27074222
Over-expression of TNC, SMA, and vimentin were significantly correlated with the lower overall survival in prostate cancer patients. PMID: 28341124
ANA and ASMA evaluation in patients with liver transplantation and no history of autoimmune disease has no clinical relevance, since it varies in time and is not related to any risk factors or liver injury. Routine autoimmunity evaluation should be avoided. PMID: 28337446
Upon actin engagement, the N-terminal "strap" and helix 1 are displaced from the vinculin tail helical bundle to mediate actin bundling. PMID: 26493222
This study reported the new information on the frequency and phenotypes of congenital myopathy caused by ACTA1 mutations in subjects >/=5 years of age. PMID: 26172852
The authors propose that Lpd delivers Ena/VASP proteins to growing barbed ends and increases their actin polymerase activity by tethering them to actin filaments. PMID: 26295568
Mutations in ACTA1 can cause pathologic features consistent with myofibrillar myopathy. PMID: 25913210
TIMP-1 significantly increased levels of alpha-SMA. PMID: 25895516
novel homozygous recessive missense variant (c.460G>C, P.(Val154Leu)) was found in two brothers with infantile-onset congenital muscular dystrophy with rigid spine. PMID: 25182138
Myopathy associated with zebra bodies is part of the spectrum of myopathies associated with the ACTA1 gene. PMID: 25747004
New scapuloperoneal phenotype associated with an ACTA1 mutation. PMID: 25938801
Antisynthetase syndrome-associated myositis is characterized by distinctive myonuclear actin filament inclusions. PMID: 25746564
The predominant pathway mediated by Australian bat lyssavirus G envelope for internalization into HEK293T cells is clathrin-and actin-dependent also requiring Rab5. PMID: 24576301
Data show that the width and speed of the waves depend counter-intuitively on parameters, negative feedback, and the F-actin time scale. PMID: 23831272
Although most cases of severe infantile form of nemaline myopathy caused by ACTA1 mutations are sporadic and have no family history PMID: 24313005
These findings suggest that the direct contact of Dengue virus 2 E protein with 43 kDa actin protein may have a crucial function in Dengue virus 2 infection of ECV304 cells. PMID: 23376163
Myotonia in adult human skeletal actin transgenic mice may be explained on the basis of a mosaic expression of ClC-1 channels in different fibres and/or on alterations of other conductances. PMID: 23247112
Respiratory muscles from an animal model of nemaline myopathy with an ACTA1 mutation show distinct muscle fiber weakness compared to limb muscles. PMID: 23656990
The objective was to evaluate the presence and distribution of the lubricating and anti-adhesion glycoprotein lubricin and cells containing the contractile isoform smooth muscle alpha-actin (SMA) in pseudomembranes around loose hip prostheses. PMID: 23174700
analysis of the pattern of of evolutionarily conserved basic and acidic residues that constitutes the binding interface of actin-tropomyosin PMID: 23420843
Data show that GnRHR activation affected several cellular markers of locomotion, including actin organization and polymerization as well as active RhoA-GTP levels. PMID: 23176180
Data indicate that actin nucleation on Chlamydia inclusions exhibited moderate depolymerization dynamics. PMID: 23071671
The organization of actin and cytokeratin cytoskeleton and the expression of TCTP, p53,cyclin A, RhoA and actin in HIO180 non-transformed ovarian epithelial cells, and OVCAR3 and SKOV3 ovarian epithelial cancer cells, was studied. PMID: 23042265
Data show that the urinary messenger RNA (mRNA) levels of alpha-smooth muscle actin (alpha-SMA), fibronectin, and matrix metalloproteinase-9 (MMP-9) were significantly higher in the diabetic nephropathy (DN) group, and mRNA levels increased with DN progression. PMID: 21824169
High alpha-actin is associated with colorectal carcinogenesis. PMID: 21912905
Over-expression of Nkx2.5 and/or cardiac alpha-actin inhibit the contraction ability of adipose tissue-derived stromal cells-derived cardiomyocytes. PMID: 21691712
Platelets gal-1 forms an intracellular complex with monomeric actin. PMID: 22081313
Herpesviruses exploit actin and actin-associated myosin motors for viral entry and virion egress. [review] PMID: 21994736
CK2 regulates vaccinia virus dissemination and actin tail formation. PMID: 22209233
Actin playes differential roles in trafficking of Epstein-Barr virus in B cells and epithelial cells. PMID: 22031939
a link between extramuscular expression of alpha-skeletal muscle actin and clinical symptoms in non-skeletal muscle tissues of patients with ACTA1 mutations, and probably a functional role of alpha-skeletal muscle actin during fetal development PMID: 21514153
The filopodium: a stable structure with highly regulated repetitive cycles of elongation and persistence depending on the actin cross-linker fascin PMID: 21975552
Actin accumulates throughout the activated immunological synapse. PMID: 21931536
Actin-EGFP and ezrin-EGFP accumulated below pilus-coated beads when force was applied. PMID: 21340023
Data show that concurrent phosphorylation of cortactin by ERK1/2 and tyrosine kinases enables cells with the ability to regulate actin dynamics. PMID: 21079800
BCL2 interaction with actin in vitro may inhibit cell motility by enhancing actin polymerization PMID: 20716950
Results suggest that actin polymerization and bundling by VASP are critical for spine formation, expansion, and modulating synaptic strength. PMID: 20826790
Centrosomal actin was detected with the anti-actin antibody 1C7 that recognizes antiparallel ("lower dimer") actin dimers. PMID: 21108927
These findings demonstrated that PI3K-mediated actin rearrangements are required for Cronobacter sakazakii invasion of human brain microvascular endothelial cells. PMID: 20809254
Results confirm that DNaseI-binding loop (D-loop) is involved in stabilization of skeletal muscle actin structure, both as monomers and filaments; actin is stabilized by ligands (by phalloidin, aluminum fluoride, & ATP [and to lesser extent by ADP]). PMID: 20718862
Simultaneous tracking of both TCR clusters and GFP-actin speckles reveals their dynamic association and individual flow patterns. Actin retrograde flow directs the inward transport of TCR clusters PMID: 20686692
We conclude that the Drosophila indirect flight muscles provide a good model system for studying ACTA1 mutations PMID: 20452215
Data demonstrate for the first time that L-plastin contributes to the fine-tuning of actin turn-over, an activity which is regulated by Ser5 phosphorylation promoting its high affinity binding to the cytoskeleton. PMID: 20169155
Podocyte BK(Ca) channels are regulated by synaptopodin, Rho, and actin microfilaments. PMID: 20630939
these data identify NHS as a new regulator of actin remodelling. PMID: 20332100
ACTA1 mutations are involved in fiber size disproportion in congenital myotonic dystrophy PMID: 20179953
First insights of S100A10 function as a regulator of the filamentous actin network. PMID: 20100475

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Involvement in disease

Nemaline myopathy 3 (NEM3); Myopathy, actin, congenital, with excess of thin myofilaments (MPCETM); Myopathy, congenital, with fiber-type disproportion (CFTD); Myopathy, scapulohumeroperoneal (SHPM)

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Families

Actin family

Database Links

HGNC: 129

OMIM: 102610

KEGG: hsa:58

STRING: 9606.ENSP00000355645

UniGene: Hs.1288

Code	CSB-EP001205HU
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Actin, alpha skeletal muscle (ACTA1)

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