Code | CSB-RA001205A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:500 |
IF | 1:30-1:200 |
CUSABIO generated the ACTA1 antibody through a multi-step process. Initially, an animal is immunized with a synthesized peptide derived from human ACTA1. B cells are isolated from the immunized animal. Selecting the ACTA1 antibody-producing B cells and identifying the single clones. The resulting ACTA1 antibody is then sequenced, and its corresponding antibody gene is synthesized. CUSABIO subsequently clones the ACTA1 antibody gene into plasma vectors, which are subsequently introduced into mammalian cells using a lipid-based transfection reagent. Following transient expression, the antibodies against ACTA1 are harvested and thoroughly characterized. The ACTA1 recombinant monoclonal antibody is purified using affinity chromatography from the culture medium. This highly purified antibody is specifically designed for the detection of human, mouse, and rat ACTA1 protein in ELISA, WB, IHC, IF, and FC applications.
There are currently no reviews for this product.