Product Details

Uniprot No.

Target Names

Alternative Names

a actin antibody; ACTA antibody; ACTA1 antibody; ACTA2 antibody; ACTC antibody; ACTC1 antibody; Actin antibody; ACTS_HUMAN antibody; ACTSA antibody; Alpha 2 actin antibody; alpha skeletal muscle antibody; Alpha-actin-1 antibody; Cardiac muscle alpha actin 1 antibody; Skeletal muscle alpha actin 1 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

25E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500
IF	1:30-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Alpha-skeletal muscle actin (ACTA1) serves as a fundamental structural protein in skeletal muscle, where it forms the thin filaments essential for muscle contraction. As a highly conserved cytoskeletal component, ACTA1 provides researchers with a reliable marker for studying muscle biology, cytoskeletal dynamics, and cellular architecture across diverse experimental systems.

This recombinant monoclonal antibody, clone 25E3, offers the consistency and reproducibility that demanding research workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, you can expect uniform performance across lots, eliminating the variability that can complicate long-term studies or multi-site collaborations. The affinity-chromatography purification ensures high specificity for your target.

Validation across multiple platforms demonstrates this antibody's versatility in your experimental toolkit. Western blot analysis confirms detection of the expected 42 kDa band across human cell lines including HeLa, A549, and HepG2, as well as mouse cell lines Raw264.7 and NIH/3T3. The antibody also performs reliably in rat brain and heart tissue lysates, providing flexibility for comparative studies. Immunohistochemistry validation in paraffin-embedded human kidney tissue shows clear staining using standard citrate buffer antigen retrieval, while immunofluorescence studies in HepG2 cells reveal distinct cytoplasmic localization patterns. Flow cytometry analysis in HeLa cells further extends the application range for quantitative single-cell studies.

With validated performance spanning Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and ELISA, this antibody supports researchers investigating cytoskeletal organization, muscle cell differentiation, and cellular marker studies where consistent, sequence-defined reagents are essential for reproducible results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA001205A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue All lanes: Actin antibody at 0.95μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa IHC image of CSB-RA001205A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-RA001205A0HU at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay histogram showing Hela cells stained with CSB-RA001205A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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ACTA1 Recombinant Monoclonal Antibody

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