Product Details

Uniprot No.

Target Names

Alternative Names

Actin, alpha skeletal muscle (Alpha-actin-1) [Cleaved into: Actin, alpha skeletal muscle, intermediate form], ACTA1, ACTA

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human ACTA1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4C1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Alpha-skeletal muscle actin (ACTA1) serves as a fundamental structural protein in skeletal muscle fibers, where it forms the thin filaments essential for muscle contraction. As a member of the highly conserved actin family, ACTA1 plays critical roles in cytoskeletal organization and cellular motility, making it a valuable marker in studies of muscle biology, cancer research, and signal transduction pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human ACTA1, offers the consistency and reliability that demanding research applications require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, you can expect exceptional lot-to-lot reproducibility throughout your long-term studies. The rabbit IgG format and affinity-chromatography purification ensure high specificity for your target.

Validation studies demonstrate robust performance across multiple experimental platforms. In immunohistochemistry, this antibody produces clear staining in paraffin-embedded human skeletal muscle tissue at dilutions of 1:50, confirming its utility for detecting ACTA1 in its native tissue context. Additionally, IHC validation in human breast cancer tissue expands its applicability to oncology research where cytoskeletal alterations may be of interest. Flow cytometry analysis using A549 cells shows distinct positive signal separation from isotype controls, indicating reliable detection of intracellular ACTA1 in fixed and permeabilized samples.

The antibody's validated performance across ELISA, immunohistochemistry, and flow cytometry provides flexibility for researchers investigating muscle-related pathologies, tumor biology, or using ACTA1 as a loading control. Whether you are characterizing tissue samples or analyzing cultured cell populations, this antibody delivers the reproducible results essential for meaningful experimental conclusions.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA289956A0HU
Size	US$210
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Image	IHC image of CSB-RA289956A0HU diluted at 1:50 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.72% DAB. IHC image of CSB-RA289956A0HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.72% DAB. Overlay Peak curve showing A549 cells stained with CSB-RA289956A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ACTA1 Recombinant Monoclonal Antibody

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