Recombinant Human Alpha-actinin-1 (ACTN1), partial

Code CSB-YP001241HU
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Source Yeast
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Code CSB-EP001241HU
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Source E.coli
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Code CSB-EP001241HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001241HU
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Source Baculovirus
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Code CSB-MP001241HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ACTN1
Uniprot No.
Alternative Names
actinin 1 smooth muscle; Actinin alpha 1; actinin; alpha 1; ACTN 1; Actn1; ACTN1_HUMAN; Alpha Actinin 1; Alpha actinin cytoskeletal isoform; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; BDPLT15; F actin cross linking protein; F-actin cross-linking protein; FLJ40884; FLJ54432; Non muscle alpha actinin 1; Non-muscle alpha-actinin-1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Gene References into Functions
  1. these results indicate that increased alpha-actinin-1 expression destabilizes E-cadherin-based adhesions, which is likely to promote the migratory potential of breast cancer cells. Furthermore, our results identify a-actinin-1 as a candidate prognostic biomarker in basal-like breast cancer. PMID: 29742177
  2. provide a structural model of regulation of the actin crosslinking activity of alpha-actinin-1 where calcium induced structural stabilisation causes fastening of the juxtaposed actin binding domain, leading to impaired capacity to crosslink actin PMID: 27272015
  3. our 11 patients with macrothrombocytopenia related to ACTN1 mutation are characterized by an isolated autosomal dominant mild thrombocytopenia, with low bleeding tendency. PMID: 28562514
  4. Calcium modulates calmodulin-ACTN1 interaction with and agonist-dependent internalization of the adenosine A2A receptor. PMID: 28130124
  5. Actinin-1 binds to the C-terminus of A2B adenosine receptor (A2BAR) and enhances A2BAR cell-surface expression PMID: 27208173
  6. This suggests that a precise dosage of PC2 is necessary for an adequate mechanosensitive alpha-actinin recruitment at junctions. PMID: 27575580
  7. The present study identified a de novo mutation in ACTA1 (c.350A>G; p.Asn117Ser) in a Chinese patient using targetcapture sequencing of a panel containing 125 known causal genes for inherited muscle diseases. PMID: 27357517
  8. Drp1 is in dynamic equilibrium on mitochondria in a fission-independent manner, and that fission factors such as actin filaments target productive oligomerization to fission sites. PMID: 26609810
  9. Host cortactin, PKD1 and actin are recruited by Trypanosoma cruzi extracellular amastigotes based on experiments in fixed and live cells by time lapse confocal microscopy. PMID: 26096820
  10. The authors show that the microtubule binding protein EB1 (end-binding protein 1), a key regulator of microtubule dynamics, can bind directly to filamentous actin (F-actin) F-actin. PMID: 26854759
  11. Several dominantly inherited congenital macrothrombocytopenia-associated mutations that are located within the actinin-1 actin-binding domain cause increased binding of actinin-1 to actin filaments and enhance filament bundling in vitro. PMID: 26879394
  12. Data indicate that alpha-actinin (110 kDa) autoantibody was characterized as a major target in lupus nephritis (LN) patients positive for Anti-membrane autoantibodies (MbA). PMID: 26071203
  13. ACTN1 determines the motility of keratinocytes by regulating the organization of the actin cytoskeleton, focal adhesion, and hemidesmosome proteins complexes, thereby modulating cell speed, lamellipodial dynamics, and directed migration PMID: 25431851
  14. ArgBP2 interaction with alpha-actinin and actin stress fibers inhibits cell migration PMID: 25429109
  15. ACTN1-related thrombocytopenia is characterized by mild course with platelet macrocytosis and low risk for bleeding. PMID: 25361813
  16. A missense mutation in the alpha-actinin 1 gene (ACTN1) is the cause of autosomal dominant macrothrombocytopenia in a large French family. PMID: 24069336
  17. The C-terminal polybasic region of CYTH2 participates in interacting directly with the EFh2 domain of ACTN1. PMID: 22659138
  18. Loss of dorsal stress fibers either by depleting alpha-actinin-1 or Rac1 results in a beta-actin accumulation at the leading edge in migrating and spreading cells. PMID: 23132927
  19. Actinin-alpha1 readily forms heterodimers composed of monomers that may have different properties and interacting proteins, altering our view of non-muscle actinin, thus altering our view of non-muscle actinin function. PMID: 23557398
  20. Transduction of mouse fetal liver-derived megakaryocytes with disease-associated ACTN1 variants caused a disorganized actin-based cytoskeleton in megakaryocytes. PMID: 23434115
  21. Alpha actinin 1, a cytoskeleton protein implicated in inflammatory/degenerative autoimmune diseases, might be regarded as a novel multiple sclerosis autoantigen. PMID: 23139387
  22. We conclude that alpha-actinin stabilizes Ca(V)1.2 at the plasma membrane and that its displacement by calcium-calmodulin triggers calcium-induced endocytosis of Ca(V)1.2, thus providing an important negative feedback mechanism for calcium influx. PMID: 23664615
  23. The alpha-actinin/EWI motif-containing protein 2 (EWI-2) complex is involved in regulation of the actin cytoskeleton at T cell immune and virological synapses, providing a link between membrane microdomains and structures involved in T cell recognition. PMID: 22689882
  24. Actinin-1 and cortactin showed matrix-contact-side localization in adenocarcinoma cells. PMID: 21474972
  25. Identification of a 31 kD amino-terminal fragment of alpha-actinin protein, named mactinin, that promotes monocyte/macrophage maturation in vitro. PMID: 10029173
  26. Mactinin is a novel inducer of Hsp90 activity on monocytes and may serve to perpetuate and augment monocytic activation. PMID: 19715605
  27. cathepsin X overexpression reduced LFA-1 clustering and induced an intermediate affinity LFA-1 conformation that is known to associate with alpha-actinin-1. PMID: 19750481
  28. the focal adhesion component alpha-actinin interacts with syndecan-4 in a beta-integrin-independent manner PMID: 12493766
  29. an alpha-actinin-dependent association between the actin cytoskeleton and A2AR trafficking PMID: 12837758
  30. the dynamics of alpha-actinin are important for PI 3-kinase-induced reorganization of the actin cytoskeleton PMID: 15710624
  31. GluR4 may regulate its synaptic targeting through phosphorylation-dependent interactions with alpha-Actinin-1 and IQGAP1 PMID: 16190873
  32. S1P-induced recruitment of S1P1 to CEM fractions promotes PI3 kinase-mediated Tiam1/Rac1 activation required for alpha-actinin-1/4-regulated cortical actin rearrangement and EC barrier enhancement PMID: 16195373
  33. Alpha-actinin plays a role in regulating cell survival through stabilization of focal adhesions and regulation of TNF-alpha-induced survival signaling. PMID: 16807302
  34. Results show that the interaction between ICAM-5 and alpha-actinin is mediated through binding of positively charged amino acids near the transmembrane domain of ICAM-5, and this interaction may play an important role in neuronal differentiation. PMID: 16820411
  35. Interaction of alpha 1 actinin with ICAM-1 is required for leukocyte extravasation. PMID: 16951376
  36. Molecular dynamics method was applied to investigate the mechanical behaviour of the human skeletal muscle alpha-actinin. PMID: 17115122
  37. there is an alpha-actinin-1-dependent mGlu(5b) receptor association with the actin cytoskeleton modulating receptor cell surface expression and functioning PMID: 17311919
  38. ACT1 is de-expressed in endometriosis and endometrioid carcinoma compared with normal uterine epithelium. PMID: 17525629
  39. Alpha-actinin-1 phosphorylation at Y12 plays a crucial role in pressure-activated cell adhesion and mechanotransduction by facilitating Src recruitment to beta(1)-integrin, and consequently the association of focal adhesion kinase with Src. PMID: 17898132
  40. Taken together, alpha-actinin not only attaches TRPP3 to the cytoskeleton but also up-regulates TRPP3 channel function. PMID: 17944866
  41. study examines the mechanism by which phosphoinositide binding regulates alpha-actinin function PMID: 17965186
  42. A subset of the tumor-specific splicing alterations (ACTN1, CALD1, and VCL) was found in all three organs and may represent general cancer-related splicing events. PMID: 18353764
  43. alpha-actinin-1 may play a role in human glomerular disease PMID: 18408146
  44. two sm-titin Zq domains interact with each other and with the two R2-R3 sites in the alpha-actinin central rod PMID: 18519573
  45. interaction of GNE with alpha-actinin 1 might point to its involvement in alpha-actinin mediated processes PMID: 18560563
  46. ICAM-2 mediates suppression of metastatic phenotype and the interaction of ICAM-2/alpha-actinin/actin represents the first complete membrane-linker protein-actin linkage to impact tumor cell motility in vitro and metastatic potential in an in vivo model. PMID: 18978946
  47. Data describe alterations of myocardial intercellular and cell-matrix contacts in hypertrophic tissue, and show intracellular translocation of beta-catenin, alpha-actinin and chondroitin sulfate proteoglycan 6 in both an animal model and in LVH patients. PMID: 19094982

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Involvement in disease
Bleeding disorder, platelet-type 15 (BDPLT15)
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. Cell membrane. Cell junction. Cell projection, ruffle.
Protein Families
Alpha-actinin family
Database Links

HGNC: 163

OMIM: 102575

KEGG: hsa:87

STRING: 9606.ENSP00000377941

UniGene: Hs.235750

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