Recombinant Human Anthrax toxin receptor 1(ANTXR1),partial,Yeast

Code CSB-YP001832HU
Size US$1298 How to order?
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names ANTXR1
Uniprot No. Q9H6X2
Research Area Tags & Cell Markers
Alternative Names Anthrax toxin receptor 1; ANTR1_HUMAN; Antxr1; ATR; Tumor endothelial marker 8
Species Homo sapiens (Human)
Source Yeast
Mol. Weight 34.4kDa
Protein Length Extracellular Domain
Tag Info N-terminal His-tagged
Tag-Free
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.; (Microbial infection) Acts as a receptor for protective antigen (PA) of B.anthracis.
Gene References into Functions
  1. Silencing TEM8 may inhibit proliferation of XWLC05 lung cancer cells, promote cell apoptosis, arrest the cell cycle at G1 phase and decrease the migration and invasive ability. PMID: 29115620
  2. Novel targets ANTXR1 and RSPO2 were confirmed to be suppressed by miR-493 directly. PMID: 28651234
  3. These studies identify ANTXR1, a class of receptor that is shared by a mammalian virus and a bacterial toxin, as the cellular receptor for Seneca Valley virus. PMID: 28650343
  4. expression does not affect cytotoxicity to anthrax toxin PMID: 27170489
  5. Findings suggest that down-regulation of tumor endothelial marker 8 play an important role in the inhibition of tumorigenesis and development of osteosarcoma. PMID: 26996335
  6. TEM8 may be differentially expressed between wound types and loss of this molecule impacts HaCaT growth and migration, potentially implicating this molecule as a factor involved in successful progression of wound healing. PMID: 26677171
  7. In the absence of any N-linked glycans, TEM8 fails to fold correctly and is recognized by the ER quality control machinery. PMID: 25781883
  8. These studies expand the allelic spectrum in this rare condition and potentially provide insight into the role of ANTXR1 in the regulation of the extracellular matrix. PMID: 25045128
  9. TEM8-targeted siRNAs also offered significant protection against lethal toxin in human macrophage-like cells. PMID: 24742682
  10. ANTXR2 is expressed by human uterine smooth muscle cell and appears important for normal human uterine smooth muscle cell viability, migration and contractility. PMID: 24060446
  11. High ANTXR1 accelerates breast tumor growth and lung metastasis. PMID: 23832666
  12. There is an attenuation of ANTXR1 expression post-infection which may be a protective mechanism that has evolved to prevent reinfection. PMID: 23607659
  13. Mutations affecting ANTXR1 function are responsible for GAPO syndrome's characteristic generalized defect in extracellular-matrix homeostasis. PMID: 23602711
  14. Two new splice variants, one encoding a membrane-bound form of the receptor and the other secreted, which we have designated V4 and V5 (the latter being the only variant expressed in the prostate). PMID: 22912819
  15. An acidic region in the cytosolic tail of ANTXR1 decreases actin association, sending a signal that prevents binding of ANTXR1 to the protective antigen and providing evidence that cytoskeletal dynamics regulate ANTXR1 function. PMID: 22303962
  16. Disruption of Tem8 results in impaired growth of human tumor xenografts of diverse origin including melanoma, breast, colon, and lung cancer. PMID: 22340594
  17. The copy number of CEA and TEM-8 mRNA, as detected by a real-time quantitative PCR, appears to be a promising marker for evaluating the risk of tumor spread. PMID: 21573768
  18. postulate that the developmentally controlled expression of TEM8 modulates endothelial cell response to canonical Wnt signaling to regulate vessel patterning and density PMID: 21829615
  19. TEM8.1 expression in breast cancer cells confers a more aggressive, proangiogenic phenotype. PMID: 22085271
  20. TEM8 was expressed at a higher level in the stroma adjacent to the triple-negative breast cancer in all cases, with focal immunoreactive areas within the tumor. PMID: 21965755
  21. studies reveal that TEM8 exists in different forms at the cell surface, a structure dependent on interactions with components of the actin cytoskeleton PMID: 21129411
  22. Data show that the two different PA oligomers are equally stabilized by ANTXR interactions. PMID: 21079738
  23. Results describe the expression, purification and crystallization of human anthrax toxin receptor 1 vWA domain to 1.8 A resolution from a single crystal. PMID: 21206026
  24. the crystal structure of the TEM8 extracellular vWA domain at 1.7 A resolution. PMID: 20585457
  25. actin was also found to be essential for efficient heptamerization of anthrax toxin PA, but only when bound to one of its 2 receptors, TEM8 PMID: 20221438
  26. Here we describe the cloning of the human PA receptor using a genetic complementation approach PMID: 11700562
  27. This is the first demonstration that the ATR/TEM8 protein is highly expressed in epithelial cells, suggesting that the ATR/TEM8 expression pattern is highly relevant for understanding the pathogenesis of anthrax infection. PMID: 15689409
  28. results indicate that TEM8 plays a positive role in endothelial cell activities related to angiogenesis PMID: 15777794
  29. These results suggest that the TEM-8 vW and transmembrane domains may play an important biological role in TEM-8 related tubule formation. PMID: 15993844
  30. because protective antigen binds to CMG2 with much higher affinity than it does to TEM8, a lower pH is needed to attenuate CMG2 binding to allow pore formation; toxin can form pores at different points in the endocytic pathway PMID: 16141341
  31. Data show that cells expressing palmitoylation-defective mutant receptors are less sensitive to anthrax toxin due to a lower number of surface receptors as well as premature internalization of protective antigen without a requirement for heptamerization. PMID: 16401723
  32. TEM8 is a new adhesion molecule linking collagen I or PA to the actin cytoskeleton PMID: 16762926
  33. TEM8 expression levels in DC-based therapeutic vaccines would allow the selection of a subgroup of patients who are most likely to benefit from therapeutic vaccination. PMID: 19440709
  34. ANTXR1 does not use an adaptor to bind the cytoskeleton. This peptide orders actin filaments into arrays, demonstrating an actin bundling activity that is novel for a membrane protein PMID: 19817382
  35. ATR/TEM8 protein is highly expressed in epithelial cells, which represent the primary location for bacterial invasion. PMID: 15689409

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Involvement in disease Hemangioma, capillary infantile (HCI); GAPO syndrome (GAPO)
Subcellular Location Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell projection, filopodium membrane; Single-pass type I membrane protein.
Protein Families ATR family
Tissue Specificity Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
Database Links

HGNC: 21014

OMIM: 230740

KEGG: hsa:84168

STRING: 9606.ENSP00000301945

UniGene: Hs.165859

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