Recombinant Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1)

Code CSB-YP622985HU
MSDS
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Source Yeast
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Code CSB-EP622985HU
MSDS
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Source E.coli
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Code CSB-EP622985HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP622985HU
MSDS
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Source Baculovirus
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Code CSB-MP622985HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ACAP1
Uniprot No.
Alternative Names
ACAP 1; ACAP1; ACAP1_HUMAN; Arf GAP with coiled coil ANK repeat and PH domains 1; Arf GAP with coiled coil; ankyrin repeat; and pleckstrin homology domains 1; Arf-GAP with coiled-coil; ANK repeat and PH domain-containing protein 1; ArfGAP with coiled-coil; ankyrin repeat and PH domains 1; CENT B1; Centaurin beta 1; Centaurin-beta-1; CENTB 1; Cnt b1; Cnt-b1; Cntb1; KIAA0050
Species
Homo sapiens (Human)
Expression Region
1-740
Target Protein Sequence
MTVKLDFEEC LKDSPRFRAS IELVEAEVSE LETRLEKLLK LGTGLLESGR HYLAASRAFV VGICDLARLG PPEPMMAECL EKFTVSLNHK LDSHAELLDA TQHTLQQQIQ TLVKEGLRGF REARRDFWRG AESLEAALTH NAEVPRRRAQ EAEEAGAALR TARAGYRGRA LDYALQINVI EDKRKFDIME FVLRLVEAQA THFQQGHEEL SRLSQYRKEL GAQLHQLVLN SAREKRDMEQ RHVLLKQKEL GGEEPEPSLR EGPGGLVMEG HLFKRASNAF KTWSRRWFTI QSNQLVYQKK YKDPVTVVVD DLRLCTVKLC PDSERRFCFE VVSTSKSCLL QADSERLLQL WVSAVQSSIA SAFSQARLDD SPRGPGQGSG HLAIGSAATL GSGGMARGRE PGGVGHVVAQ VQSVDGNAQC CDCREPAPEW ASINLGVTLC IQCSGIHRSL GVHFSKVRSL TLDSWEPELV KLMCELGNVI INQIYEARVE AMAVKKPGPS CSRQEKEAWI HAKYVEKKFL TKLPEIRGRR GGRGRPRGQP PVPPKPSIRP RPGSLRSKPE PPSEDLGSLH PGALLFRASG HPPSLPTMAD ALAHGADVNW VNGGQDNATP LIQATAANSL LACEFLLQNG ANVNQADSAG RGPLHHATIL GHTGLACLFL KRGADLGARD SEGRDPLTIA METANADIVT LLRLAKMREA EAAQGQAGDE TYLDIFRDFS LMASDDPEKL SRRSHDLHTL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Gene References into Functions
  1. The serum lever of Alzheimer's disease were decrease and the expression of ACAP1 strongly correlated with the Mini-Mental State Examination scores of the AD patients PMID: 25502766
  2. ACAP1 and ARAP2 each colocalize with Arf6 but they did not colocalize with each other and have opposing effects on focal adhesions. PMID: 25225293
  3. CENTB1 was up-regulated in patients with active UC as compared to UC remission and healthy control groups suggesting that it is involved in the inflammatory process. PMID: 23317930
  4. phosphorylation of ACAP1 relieves a localized mechanism of autoinhibition in regulating cargo binding PMID: 22645133
  5. the oncogenic potential of USP6 is linked to its ability to integrate cell migration and cytokinesis by regulating Arf6/ACAP1. PMID: 22188517
  6. ACAP1 promotes endocytic recycling by recognizing recycling sorting signals. PMID: 15525538
  7. CENTB1 selectively down-regulates NF-kappaB activation via NODs pathways, creating a "feedback" loop and suggesting a novel role of CENTB1 in innate immune responses to bacteria and inflammatory responses PMID: 17005562
  8. Results suggest that two ARF6 downstream molecules, PIP5K and ACAP1, function together in endosomal tubulation and that phosphoinositide levels may regulate endosomal dynamics. PMID: 17010122
  9. Results suggest that ACAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of a novel clathrin coat complex that is regulated by ARF6 for endocytic recycling in two key physiological settings. PMID: 17664335
  10. ACAP1 has robust, constitutive Arf6 GAP activity in vivo, with little activity toward Arf1. PMID: 18003747
  11. Centaurin beta1/KIAA0050, is a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins. Binding of phosphoinositides to a pleckstrin homology domain may regulate subcellular localisation and activity. PMID: 11050434
  12. Centaurin beta1/KIAA0050, or ACAP1, is a phospholipid dependent, ARF6 preferring GTPase-activating protein. It can regulate cytoskeletal and membrane trafficking events in the cell periphery and may be regulated via a phosphoinositide-binding PH domain. PMID: 11062263

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Subcellular Location
Recycling endosome membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity
Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
Database Links

HGNC: 16467

OMIM: 607763

KEGG: hsa:9744

STRING: 9606.ENSP00000158762

UniGene: Hs.337242

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