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Recombinant Human Astacin-like metalloendopeptidase (ASTL)

Recombinant Human Astacin-like metalloendopeptidase (ASTL)

Code	CSB-EP760708HU
MSDS	MSDS
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP760708HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ASTL. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP760708HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ASTL.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ASTL

Uniprot No.

Q6HA08

Research Area

Signal Transduction

Alternative Names

ASTLAstacin-like metalloendopeptidase; EC 3.4.-.-; Oocyte astacin; Ovastacin

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

24-431aa

Target Protein Sequence

APLASSCAGACGTSFPDGLTPEGTQASGDKDIPAINQGLILEETPESSFLIEGDIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERSTCIRFVTYQDQRDFISIIPMYGCFSSVGRSGGMQVVSLAPTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAFSRRGLPTITPLWAPSVHIGQRWNLSASDITRVLKLYGCSPSGPRPRGRGSHAHSTGRSPAPASLSLQRLLEALSAESRSPDPSGSSAGGQPVPAGPGESPHGWESPALKKLSAEASARQPQTLASSPRSRPGAGAPGVAQEQSWLAGVSTKPTVPSSEAGIQPVPVQGSPALPGGCVPRNHFKGMSED
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

50.6 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

I am a pharmaceutical company and is interested in Recombinant Human Astacin-like metalloendopeptidase(ASTL)
For my project he needs a high purity and native material. I also need all the information you have about the purification steps, activity test and so on. I want to know which kind of expression you can recommend me

Thanks for your inquiry.
Our purity standard is over 85% (SDS-PAGE test). If you need higher purity, pls tell us, we will try our best to meet your needs.
As to the purification method, we usually use the Ni column Niaffinity chromatography.
As to the activity, we haven't detected, can't guarantee the activity. We produce the protein in the mild condition, it have activty in theory.
Pls refer: http://www.jbc.org/content/279/25/26627.long

Target Background

Function

Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.

Gene References into Functions

The SAS1B metalloproteinase is proposed as a prototype cancer-oocyte tumor surface neoantigen for development of targeted immunotherapeutics with limited on-target/off tumor effects predicted to be restricted to the population of growing oocytes. PMID: 26327203
SAS1B protein [ovastacin] shows temporal and spatial restriction to oocytes in several eutherian orders and initiates translation at the primary to secondary follicle transition. PMID: 24038607
Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
cloning and characterization; could play in mammals a physiological function similar to that performed by hatching proteases in evolutionary distant species from arthropods to fish PMID: 15087446

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Subcellular Location

Cytoplasm. Cell membrane. Cytoplasmic vesicle, secretory vesicle, Cortical granule.

Protein Families

Peptidase M12A family

Tissue Specificity

Expressed in promyelocytic leukemia HL-60 cells, Burkitt's lymphoma Raji cells, and also in some ovarian carcinomas. Not detected in normal tissues.

Database Links

HGNC: 31704

OMIM: 608860

KEGG: hsa:431705

STRING: 9606.ENSP00000343674