Recombinant Human Beta-enolase (ENO3), partial

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Code	CSB-RP131674h
Abbreviation	Recombinant Human ENO3 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ENO3

Uniprot No.

P13929

Research Area

Metabolism

Alternative Names

2 phospho D glycerate hydro lyase; 2 phospho D glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; Beta enolase; Beta-enolase; ENO3; ENOB_HUMAN; Enolase 3 (beta muscle); Enolase 3; Enolase3; GSD13; MSE; Muscle specific enolase; Muscle-specific enolase; Skeletal muscle enolase

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

7-432aa

Target Protein Sequence

FAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

50.1kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Dive into the complex world of metabolic research with our high-quality Recombinant Human ENO3 protein. Beta-enolase, also known as 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, or Skeletal muscle enolase, plays a vital role in glycolysis and glucose metabolism. This protein is essential for energy production in skeletal muscles and serves as an important biomarker in various pathological conditions.

Our Recombinant Human ENO3 protein, expressed in E. coli, features a partial construct that covers the 7-432aa region, ensuring the availability of crucial domains for your metabolic research. The N-terminal 6xHis-tag facilitates efficient protein purification and identification, simplifying your experimentation process. With a purity greater than 90% as determined by SDS-PAGE, our Recombinant Human ENO3 protein is supplied in a lyophilized powder format, guaranteeing you a high-quality product for your research needs.

Customer Reviews and Q&A

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Target Background

Function

Appears to have a function in striated muscle development and regeneration.

Gene References into Functions

Molecular genetic analysis of ENO3 gene revealed two novel homozygous missense mutations, (p.Asn151Ser and p.Glu187Lys)in patients presenting with recurrent rhabdomyolysis. PMID: 25267339
Pyridoxamine and carnosine protected enolase against the total loss of catalytic activity. PMID: 21347838

Involvement in disease

Glycogen storage disease 13 (GSD13)

Subcellular Location

Cytoplasm.

Protein Families

Enolase family

Tissue Specificity

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Database Links

HGNC: 3354

OMIM: 131370

KEGG: hsa:2027

STRING: 9606.ENSP00000324105

UniGene: Hs.224171

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