Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
2 phospho D glycerate hydro lyase; 2 phospho D glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; Beta enolase; Beta-enolase; ENO3; ENOB_HUMAN; Enolase 3 (beta muscle); Enolase 3; Enolase3; GSD13; MSE; Muscle specific enolase; Muscle-specific enolase; Skeletal muscle enolase
Species
Homo sapiens (Human)
Expression Region
7-432aa
Target Protein Sequence
FAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Dive into the complex world of metabolic research with our high-quality Recombinant Human ENO3 protein. Beta-enolase, also known as 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, or Skeletal muscle enolase, plays a vital role in glycolysis and glucose metabolism. This protein is essential for energy production in skeletal muscles and serves as an important biomarker in various pathological conditions.
Our Recombinant Human ENO3 protein, expressed in E. coli, features a partial construct that covers the 7-432aa region, ensuring the availability of crucial domains for your metabolic research. The N-terminal 6xHis-tag facilitates efficient protein purification and identification, simplifying your experimentation process. With a purity greater than 90% as determined by SDS-PAGE, our Recombinant Human ENO3 protein is supplied in a lyophilized powder format, guaranteeing you a high-quality product for your research needs.
