Recombinant Human C-terminal-binding protein 1 (CTBP1)

Code CSB-YP618772HU
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Source Yeast
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Code CSB-EP618772HU
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Source E.coli
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Code CSB-EP618772HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP618772HU
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Source Baculovirus
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Code CSB-MP618772HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CTBP1
Uniprot No.
Alternative Names
BARS; brefeldin A- ribosylated substrate; C terminal binding protein 1; C-terminal-binding protein 1; CTBP; CtBP1; CTBP1_HUMAN; MGC104684
Species
Homo sapiens (Human)
Expression Region
1-440
Target Protein Sequence
MGSSHLLNKG LPLGVRPPIM NGPLHPRPLV ALLDGRDCTV EMPILKDVAT VAFCDAQSTQ EIHEKVLNEA VGALMYHTIT LTREDLEKFK ALRIIVRIGS GFDNIDIKSA GDLGIAVCNV PAASVEETAD STLCHILNLY RRATWLHQAL REGTRVQSVE QIREVASGAA RIRGETLGII GLGRVGQAVA LRAKAFGFNV LFYDPYLSDG VERALGLQRV STLQDLLFHS DCVTLHCGLN EHNHHLINDF TVKQMRQGAF LVNTARGGLV DEKALAQALK EGRIRGAALD VHESEPFSFS QGPLKDAPNL ICTPHAAWYS EQASIEMREE AAREIRRAIT GRIPDSLKNC VNKDHLTAAT HWASMDPAVV HPELNGAAYR YPPGVVGVAP TGIPAAVEGI VPSAMSLSHG LPPVAHPPHA PSPGQTVKPE ADRDHASDQL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Gene References into Functions
  1. F-box only protein 32 (FBXO32) directly ubiquitinates C-terminal binding protein 1 (CtBP1), which is required for its stability and nuclear retention. PMID: 29142217
  2. As part of a complex including PI4KIIIbeta, a 14-3-3gamma dimer, as well as to ARF and the PKD and PAK kinases, BARS binds to and activates a trans-Golgi lysophosphatidic acid (LPA) acyltransferase type delta (LPAATdelta) that converts LPA into phosphatidic acid (PA); and that this reaction is essential for fission of post-Golgi transport carriers. PMID: 27401954
  3. through targeting CtBP1-mediated suppression of the Epithelial-mesenchymal transition process, miR-644a might suppress the tumor metastasis of gastric cancer cells PMID: 27983935
  4. Overall, our findings reveal that CtBP1/2 is essential to promote to human glioma cell growth through maintaining the DNA stability regulated by the MRN/ATR/Chk1/CDK2/HIF-1alpha signaling pathway. PMID: 27699603
  5. A review of CtBP structure, role in tumor progression, and discovery and development of CtBP inhibitors that target CtBP's dehydrogenase activity and other functions, with a focus on the theory and rationale behind the designs of current inhibitors. [review] PMID: 28532298
  6. miR-644a/CTBP1/p53 have roles in suppressing breast cancer drug resistance by inhibiting cell survival and epithelial-mesenchymal transition PMID: 27409664
  7. Study reports a recurrent de novo C-terminal binding protein 1 (CTBP1) mutation is associated with developmental delay, hypotonia, ataxia, and tooth enamel defects. This is the first report of mutations within CTBP1 in association with any human disease. PMID: 27094857
  8. Pinin and CtBP1 and CtBP2 are oncotargets that closely interact with each other to regulate transcription and pre-mRNA alternative splicing and promote cell adhesion and other epithelial characteristics of ovarian cancer cells. PMID: 26871283
  9. the importance of the oligomeric state of CtBP for coactivation of NeuroD1-dependent transcription, was investigated. PMID: 27880001
  10. C-terminal binding protein 1 is a critical factor linking changes in cell metabolism to cell phenotype in hypoxic and other forms of pulmonary hypertension PMID: 27562971
  11. CtBP1 increased breast tumor growth in MeS mice modulating multiple genes and miRNA expression implicated in cell proliferation, progenitor cells phenotype, epithelial to mesenchymal transition, mammary development and cell communication in the xenografts. PMID: 26933806
  12. These data uncovered that CtBP1 protein is a valuable marker of glioma pathogenic process and that CtBP1 can serve as a novel prognostic marker for glioma therapy. PMID: 27160109
  13. human chorionic gonadotropin stimulated miR-212, which down-regulated OLFM1 and CTBP1 expression in fallopian and endometrial epithelial cells to favor spheroid attachment PMID: 26377223
  14. It is a putative target gene of miR-137 in breast neoplasms. PMID: 26337822
  15. CtBP physically interacted with TCF-4, and this interaction was significantly inhibited in the presence of MTOB. The above findings point to a novel role of CtBPs in the promotion of CSC growth and self-renewal PMID: 25483087
  16. High CtBP1 expression is associated with Prostate tumor. PMID: 24842953
  17. C-terminal-binding protein (CtBP) was found to have an essential role in promoting glutaminolysis by directly repressing the expression of SIRT4. PMID: 25633289
  18. MCRIP1, an ERK substrate, mediates ERK-induced gene silencing during epithelial-mesenchymal transition by regulating the co-repressor CtBP. PMID: 25728771
  19. Transactivation of Ctbp was dependent on the histone H3 lysine 9 (H3K9) demethylase activity of LSD1 facilitates subsequent H3K9 acetylation by the NeuroD1-associated histone acetyltransferase, P300/CBP-associated factor. PMID: 24732800
  20. Crystal structures of human CtBP1 and CtBP2 in complex with 4-Methylthio 2-oxobutyric acid and NAD. PMID: 24657618
  21. A transgenic model suggests transcriptional activities of CtBP1 for epithelial mesenchymal interplay and a possibility of a pathogenic role in hair follicle morphogenesis and differentiation. PMID: 24280726
  22. CtBP1 was upregulated in HCC. PMID: 23756565
  23. High CTBP1 expression is associated with gastric cancer. PMID: 23907728
  24. dinucleotide binding permits CtBP1 to form an intranuclear homodimer through a Trp(318) switch, creating a nucleation site for multimerization through the C-terminal domain for tetramerization to form an effective repression complex PMID: 23940047
  25. Interaction of E1A with importin alpha3/Qip1, dual-specificity tyrosine-regulated kinase 1A (DYRK1A), HAN11, and CtBP influenced transformation with E1B-55K. PMID: 23864635
  26. Authors found that PLEIAD also interacts with CTBP1 (C-terminal binding protein 1), a transcriptional co-regulator, and CTBP1 is proteolyzed in COS7 cells expressing CAPN3. PMID: 23707407
  27. Interaction with CtBP suppresses the immortalization activity of adenovirus E1A in primary epithelial cells and is needed for efficient virus replication during productive infecction. PMID: 23747199
  28. Data show that ADP-ribosylation of CtBP1-S/BARS by brefeldin A (BFA) occurs via synthesis of a BFA-ADP-ribose conjugate by the ADP-ribosyl cyclase CD38 and covalent binding of the BFA-ADP-ribose conjugate into the CtBP1-S/BARS NAD(+)-binding pocket. PMID: 23716697
  29. These findings provide connections of AMPK with CtBP1-mediated regulation of Bax expression for cell death under metabolic stresses. PMID: 23291169
  30. CtBP1 is expressed in melanoma and represses the transcription of p16INK4a and Brca1. PMID: 23303449
  31. These findings define broad roles for CtBP in breast cancer biology. PMID: 23385593
  32. High CtBP1 expression is associated with prostate cancer progression. PMID: 23097625
  33. CtBP is expressed in adenohypophyseal cells and is expressed at high levels in human corticotroph, somatotroph, and lactotroph pituitary adenomas PMID: 22301782
  34. CtBP1 down-regulates Brca1 and E-cadherin genes in human breast cancer. PMID: 21681822
  35. CtBP1 and CtBP2 promote the oligomerization of truncated APC through binding to the 15 amino acid repeats of truncated APC. PMID: 21665989
  36. In breast tumours, both major CTBP1 mRNA splice forms are variably expressed. PMID: 20964627
  37. CtBP1 represses Brca1 transcription by binding to the E2F4 site of the Brca1 promoter. The recruitment of CtBP1 to the Brca1 promoter increased at high NADH levels in hypoxic conditions. PMID: 20818429
  38. The Tel-CtBP complex conditions endothelial cells for angiogenesis by controlling the balance between stimulatory and antagonistic sprouting cues. PMID: 20835243
  39. this study reveals a novel combinatorial role for Bcl3 and CtBP1, providing an explanation for the acquisition of resistance to apoptosis in cancer cells, which is a major requirement for cancer development. PMID: 20800578
  40. Co-expression of Pc2 and Akt1 results in both phosphorylation and ubiquitylation of CtBP1, thereby targeting CtBP1 for degradation. PMID: 20361981
  41. CtBP proteins repress transcription in a histone deacetylase dependent or independent manner. PMID: 11864595
  42. Interaction with CtBP was shown to be important in the repression of transcription by EBNA3A and in the ability of EBNA3A to immortalize and transform primary cells PMID: 12372828
  43. biochemical and crystallographic studies reveal that CtBP, a transcription corepressor, is a functional NAD(+)-regulated dehydrogenase. PMID: 12419229
  44. CtBP co-repressor complex mediates coordinated histone modifications PMID: 12700765
  45. The corepressor C-terminal-binding protein binds the MLL repression domain. PMID: 12829790
  46. Smad6 repressed bone morphogenetic protein-induced Id1 transcription through recruiting transcriptional corepressor C-terminal binding protein (CtBP). PMID: 14645520
  47. the interaction of Pnn with the corepressor CtBP1 may modulate repression of E-cadherin transcription by CtBP1 PMID: 15542832
  48. AML1-FOG2 and FOG2-AML1 are expressed in myelodysplastic syndrome; results suggest a central role for CtBP in AML1-FOG2 transcriptional repression and implicate coordinated disruption of AML1 and GATA developmental programs in the disease pathogenesis PMID: 15705784
  49. Homeodomain-interacting protein kinase-2 (HIPK2) mediates CtBP phosphorylation and degradation in UV-triggered apoptosis. PMID: 15708980
  50. results lead to conclusion that, in colon epithelial cells, the expression level of the K18 gene is kept in check by a repression mechanism involving CtBP1, HDAC & BRCA1; mechanism is altered in SW613-S colon carcinoma cells that overexpress the K18 gene PMID: 15831101

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
D-isomer specific 2-hydroxyacid dehydrogenase family
Tissue Specificity
Expressed in germinal center B-cells.
Database Links

HGNC: 2494

OMIM: 602618

KEGG: hsa:1487

STRING: 9606.ENSP00000290921

UniGene: Hs.208597

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