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CTBP1 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA546965A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Mouse brain tissue lysate(30µg)
    All lanes: CtBP1 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 48 kDa
    Observed band size: 48 kDa
    Exposure time：2min
  • IHC image of CSB-RA546965A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of HepG2 cell with CSB-RA546965A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Overlay Peak curve showing HepG2 cells stained with CSB-RA546965A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q13363
Target Names
CTBP1
Alternative Names
C-terminal-binding protein 1 (CtBP1) (EC 1.1.1.-), CTBP1, CTBP
Species Reactivity
Human, Mouse
Immunogen
A synthesized peptide from human CTBP1 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
11B3
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

C-terminal-binding protein 1 (CTBP1) functions as a transcriptional corepressor that plays critical roles in development, cellular differentiation, and oncogenesis. By interacting with various transcription factors, CTBP1 modulates gene expression programs involved in epithelial-mesenchymal transition, apoptosis regulation, and tumor progression, making it a significant target for researchers investigating cancer biology, developmental processes, and epigenetic regulation.

This recombinant monoclonal antibody, generated from clone 11B3, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers benefit from lot-to-lot uniformity that eliminates the variability often encountered with traditional hybridoma-derived antibodies. This consistency is particularly valuable for longitudinal studies or when establishing standardized protocols across research teams.

Validation across multiple applications demonstrates the flexibility this antibody provides for diverse experimental approaches. Western blot analysis confirms specific detection at the expected 48 kDa molecular weight across human cell lines including SH-SY5Y, HeLa, and Jurkat, with cross-species reactivity verified in mouse NIH/3T3 cells and mouse brain tissue. Immunohistochemistry staining has been validated in paraffin-embedded human glioma tissue, while immunofluorescence and flow cytometry applications have been confirmed using HepG2 cells, enabling both fixed-cell imaging and quantitative single-cell analysis.

The antibody's performance across neuronal, epithelial, and immune cell types, combined with its demonstrated utility in both human and mouse samples, supports investigations into CTBP1's diverse biological functions. Whether examining transcriptional regulation in cancer models, characterizing protein expression in neural tissues, or exploring developmental pathways, this antibody delivers the specificity and versatility needed for rigorous CTBP1 research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Gene References into Functions
  1. F-box only protein 32 (FBXO32) directly ubiquitinates C-terminal binding protein 1 (CtBP1), which is required for its stability and nuclear retention. PMID: 29142217
  2. As part of a complex including PI4KIIIbeta, a 14-3-3gamma dimer, as well as to ARF and the PKD and PAK kinases, BARS binds to and activates a trans-Golgi lysophosphatidic acid (LPA) acyltransferase type delta (LPAATdelta) that converts LPA into phosphatidic acid (PA); and that this reaction is essential for fission of post-Golgi transport carriers. PMID: 27401954
  3. through targeting CtBP1-mediated suppression of the Epithelial-mesenchymal transition process, miR-644a might suppress the tumor metastasis of gastric cancer cells PMID: 27983935
  4. Overall, our findings reveal that CtBP1/2 is essential to promote to human glioma cell growth through maintaining the DNA stability regulated by the MRN/ATR/Chk1/CDK2/HIF-1alpha signaling pathway. PMID: 27699603
  5. A review of CtBP structure, role in tumor progression, and discovery and development of CtBP inhibitors that target CtBP's dehydrogenase activity and other functions, with a focus on the theory and rationale behind the designs of current inhibitors. [review] PMID: 28532298
  6. miR-644a/CTBP1/p53 have roles in suppressing breast cancer drug resistance by inhibiting cell survival and epithelial-mesenchymal transition PMID: 27409664
  7. Study reports a recurrent de novo C-terminal binding protein 1 (CTBP1) mutation is associated with developmental delay, hypotonia, ataxia, and tooth enamel defects. This is the first report of mutations within CTBP1 in association with any human disease. PMID: 27094857
  8. Pinin and CtBP1 and CtBP2 are oncotargets that closely interact with each other to regulate transcription and pre-mRNA alternative splicing and promote cell adhesion and other epithelial characteristics of ovarian cancer cells. PMID: 26871283
  9. the importance of the oligomeric state of CtBP for coactivation of NeuroD1-dependent transcription, was investigated. PMID: 27880001
  10. C-terminal binding protein 1 is a critical factor linking changes in cell metabolism to cell phenotype in hypoxic and other forms of pulmonary hypertension PMID: 27562971
  11. CtBP1 increased breast tumor growth in MeS mice modulating multiple genes and miRNA expression implicated in cell proliferation, progenitor cells phenotype, epithelial to mesenchymal transition, mammary development and cell communication in the xenografts. PMID: 26933806
  12. These data uncovered that CtBP1 protein is a valuable marker of glioma pathogenic process and that CtBP1 can serve as a novel prognostic marker for glioma therapy. PMID: 27160109
  13. human chorionic gonadotropin stimulated miR-212, which down-regulated OLFM1 and CTBP1 expression in fallopian and endometrial epithelial cells to favor spheroid attachment PMID: 26377223
  14. It is a putative target gene of miR-137 in breast neoplasms. PMID: 26337822
  15. CtBP physically interacted with TCF-4, and this interaction was significantly inhibited in the presence of MTOB. The above findings point to a novel role of CtBPs in the promotion of CSC growth and self-renewal PMID: 25483087
  16. High CtBP1 expression is associated with Prostate tumor. PMID: 24842953
  17. C-terminal-binding protein (CtBP) was found to have an essential role in promoting glutaminolysis by directly repressing the expression of SIRT4. PMID: 25633289
  18. MCRIP1, an ERK substrate, mediates ERK-induced gene silencing during epithelial-mesenchymal transition by regulating the co-repressor CtBP. PMID: 25728771
  19. Transactivation of Ctbp was dependent on the histone H3 lysine 9 (H3K9) demethylase activity of LSD1 facilitates subsequent H3K9 acetylation by the NeuroD1-associated histone acetyltransferase, P300/CBP-associated factor. PMID: 24732800
  20. Crystal structures of human CtBP1 and CtBP2 in complex with 4-Methylthio 2-oxobutyric acid and NAD. PMID: 24657618
  21. A transgenic model suggests transcriptional activities of CtBP1 for epithelial mesenchymal interplay and a possibility of a pathogenic role in hair follicle morphogenesis and differentiation. PMID: 24280726
  22. CtBP1 was upregulated in HCC. PMID: 23756565
  23. High CTBP1 expression is associated with gastric cancer. PMID: 23907728
  24. dinucleotide binding permits CtBP1 to form an intranuclear homodimer through a Trp(318) switch, creating a nucleation site for multimerization through the C-terminal domain for tetramerization to form an effective repression complex PMID: 23940047
  25. Interaction of E1A with importin alpha3/Qip1, dual-specificity tyrosine-regulated kinase 1A (DYRK1A), HAN11, and CtBP influenced transformation with E1B-55K. PMID: 23864635
  26. Authors found that PLEIAD also interacts with CTBP1 (C-terminal binding protein 1), a transcriptional co-regulator, and CTBP1 is proteolyzed in COS7 cells expressing CAPN3. PMID: 23707407
  27. Interaction with CtBP suppresses the immortalization activity of adenovirus E1A in primary epithelial cells and is needed for efficient virus replication during productive infecction. PMID: 23747199
  28. Data show that ADP-ribosylation of CtBP1-S/BARS by brefeldin A (BFA) occurs via synthesis of a BFA-ADP-ribose conjugate by the ADP-ribosyl cyclase CD38 and covalent binding of the BFA-ADP-ribose conjugate into the CtBP1-S/BARS NAD(+)-binding pocket. PMID: 23716697
  29. These findings provide connections of AMPK with CtBP1-mediated regulation of Bax expression for cell death under metabolic stresses. PMID: 23291169
  30. CtBP1 is expressed in melanoma and represses the transcription of p16INK4a and Brca1. PMID: 23303449
  31. These findings define broad roles for CtBP in breast cancer biology. PMID: 23385593
  32. High CtBP1 expression is associated with prostate cancer progression. PMID: 23097625
  33. CtBP is expressed in adenohypophyseal cells and is expressed at high levels in human corticotroph, somatotroph, and lactotroph pituitary adenomas PMID: 22301782
  34. CtBP1 down-regulates Brca1 and E-cadherin genes in human breast cancer. PMID: 21681822
  35. CtBP1 and CtBP2 promote the oligomerization of truncated APC through binding to the 15 amino acid repeats of truncated APC. PMID: 21665989
  36. In breast tumours, both major CTBP1 mRNA splice forms are variably expressed. PMID: 20964627
  37. CtBP1 represses Brca1 transcription by binding to the E2F4 site of the Brca1 promoter. The recruitment of CtBP1 to the Brca1 promoter increased at high NADH levels in hypoxic conditions. PMID: 20818429
  38. The Tel-CtBP complex conditions endothelial cells for angiogenesis by controlling the balance between stimulatory and antagonistic sprouting cues. PMID: 20835243
  39. this study reveals a novel combinatorial role for Bcl3 and CtBP1, providing an explanation for the acquisition of resistance to apoptosis in cancer cells, which is a major requirement for cancer development. PMID: 20800578
  40. Co-expression of Pc2 and Akt1 results in both phosphorylation and ubiquitylation of CtBP1, thereby targeting CtBP1 for degradation. PMID: 20361981
  41. CtBP proteins repress transcription in a histone deacetylase dependent or independent manner. PMID: 11864595
  42. Interaction with CtBP was shown to be important in the repression of transcription by EBNA3A and in the ability of EBNA3A to immortalize and transform primary cells PMID: 12372828
  43. biochemical and crystallographic studies reveal that CtBP, a transcription corepressor, is a functional NAD(+)-regulated dehydrogenase. PMID: 12419229
  44. CtBP co-repressor complex mediates coordinated histone modifications PMID: 12700765
  45. The corepressor C-terminal-binding protein binds the MLL repression domain. PMID: 12829790
  46. Smad6 repressed bone morphogenetic protein-induced Id1 transcription through recruiting transcriptional corepressor C-terminal binding protein (CtBP). PMID: 14645520
  47. the interaction of Pnn with the corepressor CtBP1 may modulate repression of E-cadherin transcription by CtBP1 PMID: 15542832
  48. AML1-FOG2 and FOG2-AML1 are expressed in myelodysplastic syndrome; results suggest a central role for CtBP in AML1-FOG2 transcriptional repression and implicate coordinated disruption of AML1 and GATA developmental programs in the disease pathogenesis PMID: 15705784
  49. Homeodomain-interacting protein kinase-2 (HIPK2) mediates CtBP phosphorylation and degradation in UV-triggered apoptosis. PMID: 15708980
  50. results lead to conclusion that, in colon epithelial cells, the expression level of the K18 gene is kept in check by a repression mechanism involving CtBP1, HDAC & BRCA1; mechanism is altered in SW613-S colon carcinoma cells that overexpress the K18 gene PMID: 15831101

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
D-isomer specific 2-hydroxyacid dehydrogenase family
Tissue Specificity
Expressed in germinal center B-cells.
Database Links

HGNC: 2494

OMIM: 602618

KEGG: hsa:1487

STRING: 9606.ENSP00000290921

UniGene: Hs.208597

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