Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ALCAM

Uniprot No.

Q13740

Research Area

Immunology

Alternative Names

Activated leukocyte cell adhesion molecule; ALCAM; ALCAM protein; CD 166; CD166; CD166 antigen; CD166_HUMAN; FLJ3851; FLJ38514; MEMD; MGC71733

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

28-516aa

Target Protein Sequence

WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

70.8kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human ALCAM protein is made through genetic engineering, also called gene splicing or recombinant DNA technology. By putting Human ALCAM genes into the genetic material of the E.coli system. These microorganisms can be used as factories or producers to make proteins for medical, academic and research uses. DNA to be manipulated it must be placed within a “transport vehicle” in which proteins may be produced from the genetic code of the DNA. The host cells used for Human ALCAM protein synthesis are E.coli cells, the whole production processes include isolation of ALCAM gene, amplification of ALCAM gene, cloning, ALCAM gene selection, and expression, and the ALCAM protein purification, the vector contains N-terminal 6xHis-SUMO tag in addition to the specific DNA sequence, this facilitates the purification of the recombinant protein and it’s finally detected with a purity of 90%+ by SDS-PAGE.

ALCAM, also called CD166, is a cell-cell adhesion molecule that engages in heterotypic interactions via the ligation to CD6 on T cells thus acting in homophilic adhesion complexes between epithelial cells and that acts as a cell surface marker for a group of hematopoietic progenitor cells, multiple mesenchymal stem cells, and cancer stem cells. CD166 is upregulated and considered a valuable prognostic marker of disease progression and dismal survival in several categories of epithelial cancers such as colorectal carcinoma, breast cancer, and pancreatic cancer. Studies have shown that CD166 can play an anti-apoptotic role by enhancing the function of YAP and exert its pro-carcinogenic role by inhibiting FOXO proteins.

Target Background

Function

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Required for normal hematopoietic stem cell engraftment in the bone marrow. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation. Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons.; Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

Gene References into Functions

multivariate Cox hazards regression analysis identified ALCAM and PD-L1 (both P < 0.01) as potential independent risk factors for primary diffuse pleural mesotheliomas PMID: 28811252
Activated leukocyte cell adhesion molecule (ALCAM) has been implicated in tumorigenesis. PMID: 29315254
this study establishes for the first time that CD166 is the ligand of ILT3. Blockade of CD166 by ILT3.Fc inhibited progression of human tumor cell lines in NOD.Cg-Prkdc Il-2rg/SzJ mice, suggesting its potential immunotherapeutic value. PMID: 29263213
These data indicate that cardiac surgery influences the expression of CD162, CD166 and CD195 and that the intensity of the immune system response, displayed as the change in the CD162, CD166, CD195 expression, varies, depending on the surgical technique used. PMID: 27625334
These findings indicate that high ALCAM expression is associated with poor prognosis and advanced clinicopathological characteristics in CRC patients. PMID: 28537909
ALCAM is a potential mediator in the late complications of diabetes in the kidney. PMID: 28325697
we show that although the histological detection of ALCAM within the tumor tissue correlates strongly with tumor stage in BCa (Figure (Figure2),2), it does not appear to be prognostic of overall survival. In contrast, urine ALCAM correlates with tumor stage and is a significant independent predictor of 3-year overall survival for patients after cystectomy. PMID: 27894096
Our data indicate that Gal-8 interacts with ALCAM at the surface of breast cancer cells through glycosylation-dependent mechanisms. A novel heterophilic interaction between ALCAM and Gal-8 is demonstrated here, suggesting its physiologic relevance in the biology of breast cancer cells PMID: 27130882
CD166 functions as a risk factor for cancers, and the alterations of its different functional isoforms were observed to correlate with specific or interplayed clinical outcomes. PMID: 27398729
The results highlight the potential of ALCAM as a recurrent biomarker in early-stage endometrioid endometrial cancer and point to ALCAM as an important molecule in endometrial cancer dissemination by regulating cell migration, invasion, and metastasis. PMID: 27873306
MiR-148a and miR-152 can sensitize tamoxifen-resistant MCF-7 breast cancer cells to tamoxifen via downregulating ALCAM. PMID: 28063929
CHIP directly regulates the stability of CD166 protein through the ubiquitin proteasome system. PMID: 28279658
ALCAM is overexpressed on the surface of Human T-lymphotropic virus type 1-infected lymphocytes, both in chronically infected cell lines and in primary infected CD4(+) T lymphocytes. PMID: 27252538
ALCAM is upregulated in pancreatic stellate cells of pancreatic cancer tissues, suggesting a potential role of ALCAM in regulating pancreatic cancer cell-pancreatic stellate cells interactions. PMID: 27573419
PRMT1 is overexpressed in human melanoma, and may regulate tumor growth and metastasis via targeting ALCAM. PMID: 27175582
SFMSCs increased through upregulation of the activated lymphocyte cell adhesion molecule (ALCAM) and N-cadherin by microRNA-192 and -218 downregulation, similar to BMMSCs and ADMSCs. PMID: 28039611
Analysis of KRAS mutation combined with immunohistochemical expression of CD44 and CD166 identified subgroups of patients with colon adenocarcinoma at higher risk of lymph node involvement by the tumor and development of liver and lung metastasis. PMID: 27062566
Weak ALCAM expression was significantly correlated with established markers for poor prognosis, as well as shorter RFS and OS. ALCAM might be considered as a prognostic marker for infantile neuroblastoma PMID: 27466504
CD166 antigen is specifically expressed on corneal endothelial cells. PMID: 26902886
Prognostic Significance of CD44v6, CD133, CD166, and ALDH1 Expression in Small Intestinal Adenocarcinoma. PMID: 25710579
The binding sites on CD6 and CD166 have been characterized to show that a SNP in CD6 causes glycosylation that hinders the CD6/CD166 interaction. PMID: 26146185
a model that CD166 regulates MCAM through a signaling flow from activation of PI3K/AKT and c-Raf/MEK/ERK signaling to the inhibition of potential MCAM ubiquitin E3 ligases, betaTrCP and Smurf1 PMID: 26004137
High ALCAM expression in melanoma cells of the primary tumor may indicate a more invasive phenotype. Low expression of ALCAM in regional lymph node metastases is a feature associated with unfavorable prognosis in patients with cutaneous melanoma. PMID: 26134500
High ALCAM expression is associated with Invasive Cholangiocarcinoma. PMID: 25987048
The Serum CD166 is a novel diagnostic tumor marker for hepatocellular carcinoma (HCC). PMID: 25562819
Overexpression of activated leukocute cell adhesion molecule in gastric cancer is associated with advanced stages and poor prognosis and miR-9 deregulation PMID: 25395097
Differentially expressed/regulated cancer-related genes upon miR-125b expression along with the significant increase of ALCAM PMID: 25539763
Low expression of ALCAM at sites of cell-cell contact in primary breast cancer tumors regardless of differentiation, size and lymph node involvement may contribute to the more aggressive phenotype of breast cancer among African-American women. PMID: 25255861
an overexpression of CD166 was detected in the benign and malignant salivary gland tumors and its expression in the malignant tumor was associated with the aggressive behavior and tumor progression. PMID: 25472586
ALCAM is generally expressed in normal and cancerous breast epithelium and that a marked reduction of ALCAM expression characterizes a subset of breast cancer patients with adverse tumor characteristics and unfavorable clinical outcome. PMID: 25270339
Authors propose a framework for how ALCAMs contribute to DC-T-cell adhesion, stabilize DC-T-cell contacts and form a mechanical link between CD6 and the actin cortex to strengthen cell adhesion at the immunological synapse. PMID: 24496453
CD14(+)CD16(+) monocytes selectively transmigrated across our BBB model as a result of their increased JAM-A and ALCAM expression. PMID: 25420915
miR126-5p is a functional, endothelial-enriched microRNA that participates in the control of leucocyte trafficking by regulating the expression of ALCAM and SetD5. PMID: 24562769
Migration of CD166 positive progenitor cells to sites of cartilage damage may be directed by regulation of DCC/CREB signaling. PMID: 24966904
The expression of CD166 using immunohistochemistry in a large cohort of non-small cell lung cancer patients , was evaluated. PMID: 24501004
These show high p21 and CD166 expression at the pretreatment biopsy were associated with tumor regression and poor prognosis in patients treated with 5-FU based chemoradiotherapy PMID: 24708484
High ALCAM expression is associated with brain metastasis. PMID: 24311639
An antibody drug conjugate was generated recognizing the CD166 antigen which was found to be strongly up-regulated in all AML cell lines and AML blasts of some patients PMID: 24487095
CD166 affected-CD44 expression is dependent of transcription via blocking NF-kappaB pathway. On the contrary, CD44 promoted up-regulation of CD166 mRNA and protein. PMID: 25094049
By directly targeting the Rho GDP dissociation inhibitor alpha (RhoGDI1) and activated leukocyte cell adhesion molecule (ALCAM). PMID: 24710410
ALCAM stably interacts with actin by binding to syntenin-1 and ezrin. PMID: 24662291
this work summarizes a novel link between CD166 and YAP, explores the interplay among related important signaling pathways, and may lead to more effective therapeutic strategies for liver cancer. PMID: 24482231
Patients whose head and neck squamous cell carcinoma tumors expressed high levels of CD166 had a significantly poorer clinical outcome than those whose tumors expressed low levels of CD166. PMID: 23903875
ALCAM expression and shedding is elevated in response to TGF-beta signaling. PMID: 24385212
The entry of HIV infected and uninfected CD14(+)CD16(+) monocytes into the brain was facilitated by significantly increased surface JAM-A, ALCAM, CD99, and PECAM-1, as compared to CD14(+) cells that are CD16 negative. PMID: 23922698
Data indicate that arylsulfonamide inhibited ADAM-17 with selectivity, and maintained inhibitory properties on sALCAM shedding. PMID: 24044434
CD166 expression indicate advanced tumor load category and node-positive status in colorectal cancer specifically. [Meta-analysis] PMID: 23940674
Loss of E-cadherin and beta-catenin with cytoplasmic ALCAM accumulation may play pivotal role in oral cancer development and progression. PMID: 23840677
ALCAM can be taken forward for analysis in sera of patients with thyroid carcinoma to determine its applicability as a minimally invasive serum biomarker for TC aggressiveness and patient disease-free survival. PMID: 23148625
retention of intact ALCAM was associated with improved survival PMID: 23539446

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite.; [Isoform 3]: Secreted.

Tissue Specificity

Detected on hematopoietic stem cells derived from umbilical cord blood. Detected on lymph vessel endothelial cells, skin and tonsil. Detected on peripheral blood monocytes. Detected on monocyte-derived dendritic cells (at protein level). Detected at low l

Database Links

HGNC: 400

OMIM: 601662

KEGG: hsa:214

STRING: 9606.ENSP00000305988

UniGene: Hs.591293

Code	CSB-EP614444HU
Abbreviation	Recombinant Human ALCAM protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human CD166 antigen (ALCAM), partial

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