Greater than 90% as determined by SDS-PAGE.
Activated leukocyte cell adhesion molecule; ALCAM; ALCAM protein; CD 166; CD166; CD166 antigen; CD166_HUMAN; FLJ3851; FLJ38514; MEMD; MGC71733
Homo sapiens (Human)
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The recombinant Human ALCAM protein is made through genetic engineering, also called gene splicing or recombinant DNA technology. By putting Human ALCAM genes into the genetic material of the E.coli system. These microorganisms can be used as factories or producers to make proteins for medical, academic and research uses. DNA to be manipulated it must be placed within a “transport vehicle” in which proteins may be produced from the genetic code of the DNA. The host cells used for Human ALCAM protein synthesis are E.coli cells, the whole production processes include isolation of ALCAM gene, amplification of ALCAM gene, cloning, ALCAM gene selection, and expression, and the ALCAM protein purification, the vector contains N-terminal 6xHis-SUMO tag in addition to the specific DNA sequence, this facilitates the purification of the recombinant protein and it’s finally detected with a purity of 90%+ by SDS-PAGE.
ALCAM, also called CD166, is a cell-cell adhesion molecule that engages in heterotypic interactions via the ligation to CD6 on T cells thus acting in homophilic adhesion complexes between epithelial cells and that acts as a cell surface marker for a group of hematopoietic progenitor cells, multiple mesenchymal stem cells, and cancer stem cells. CD166 is upregulated and considered a valuable prognostic marker of disease progression and dismal survival in several categories of epithelial cancers such as colorectal carcinoma, breast cancer, and pancreatic cancer. Studies have shown that CD166 can play an anti-apoptotic role by enhancing the function of YAP and exert its pro-carcinogenic role by inhibiting FOXO proteins.