Code | CSB-RA437652A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:2000 |
IHC | 1:50-1:200 |
The process of generating the ALCAM recombinant monoclonal antibody is complex and requires several stages. Firstly, the ALCAM monoclonal antibody is obtained and its gene sequence is determined. Next, a vector containing the ALCAM monoclonal antibody gene is created and introduced into a host cell line for culture. During the production of the ALCAM monoclonal antibody, a synthesized peptide based on human ALCAM is utilized as the immunogen. Affinity chromatography is then employed to purify the resulting ALCAM recombinant monoclonal antibody, which is further evaluated for specificity using ELISA, WB, and IHC applications. This ALCAM recombinant monoclonal antibody is reactive with human and mouse ALCAM proteins.
ALCAM, also known as CD166, is a type I transmembrane protein that belongs to the immunoglobulin superfamily. ALCAM is expressed in various cell types such as lymphocytes, monocytes, endothelial cells, and certain epithelial cells. Its main function is to mediate cell-to-cell adhesion by binding to different receptors such as CD6, CD166, and integrin αvβ3. ALCAM is involved in various biological processes including cell migration, lymphocyte activation, and differentiation, and is also implicated in various diseases such as cancer and autoimmune disorders.
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