Recombinant Human Carbonyl reductase [NADPH] 3 (CBR3)

Code CSB-YP004587HU
MSDS
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Source Yeast
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Code CSB-EP004587HU
MSDS
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Source E.coli
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Code CSB-EP004587HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004587HU
MSDS
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Source Baculovirus
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Code CSB-MP004587HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CBR3
Uniprot No.
Alternative Names
Carbonyl reductase (NADPH) 3; Carbonyl reductase (NADPH) 3 EC 1.1.1.184; Carbonyl reductase [NADPH] 3; Carbonyl reductase 3; CBR 3; CBR3; CBR3_HUMAN; EC 1.1.1.184; Epididymis secretory protein Li 25; hCBR3; HEL S 25; NADPH dependent carbonyl reductase 3; NADPH-dependent carbonyl reductase 3; SDR21C2; Short chain dehydrogenase/reductase family 21C member 2
Species
Homo sapiens (Human)
Expression Region
1-277
Target Protein Sequence
MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET PVYLALLPPD ATEPQGQLVH DKVVQNW
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).
Gene References into Functions
  1. CBR3 730G>A is significantly associated with recurrence- free survival in patients who received intravesical chemotherapy with pirarubicin after complete transurethral resection. PMID: 30125887
  2. No significant correlation between cardiotoxicity and SNPs within the CBR pathway. Further investigation into CBR SNPs in a larger adult sample is needed. PMID: 26563179
  3. association of SNPs in ABCB1 and CBR3 with chronic cardiotoxicity in a cohort of breast cancer patients treated with anthracyclines. PMID: 26799497
  4. Variants of CBR3 and GSTP1 enzymes may be associated with changes in short-term functional cardiac parameters. PMID: 23182048
  5. genetic polymorphism in the CBR3 gene conferred risk of type 2 diabetes and insulin resistance in Chinese. The association was probably mediated through modulation of adipogenesis. PMID: 22527884
  6. CBR3 polymorphisms contribute to increased cardiomyopathy risk associated with anthracycline treatment of childhood cancer. PMID: 22124095
  7. CBR3 is a novel target gene of inflammatory stimuli; elucidation of its detailed role in inflammation deserves further investigation. PMID: 22425771
  8. Computational searches identify a conserved antioxidant response element(ARE) in the distal carbonyl reductase 3 (CBR3) promoter region. PMID: 22001310
  9. The CBR3 distal promoter contains an activating cis-regulatory element that is responsive to Trichostatin A (TSA) treatment according to promoter reporter assays in HEK 293 cells. PMID: 20494980
  10. CBR3 is regulated via NRF2-dependent signaling pathways, a finding and plays an important role in the cellular response to oxidative stress. PMID: 20806931
  11. analysis of the structural basis for substrate specificity in human monomeric carbonyl reductases CBR3 PMID: 19841672
  12. The common carbonyl reductase 3 (CBR3)valine244methionine polymorphism encodes for CBR3 isoforms with distinctive enzymatic properties, as revealed in DNA variation panels from 10 ethnic groups. PMID: 15537833
  13. There was a trend toward an association between the CBR3 V244M polymorphism and the risk of CHf PMID: 18457324
  14. Polymorphisms in CBR3 may explain interindividual and interethnic variability of doxorubicin pharmacokinetics and pharmacodynamics. PMID: 18551042
  15. CBR3 polymorphisms have no significant influence on the pharmacokinetics of doxorubicin in Asian breast cancer patients. PMID: 19016765
  16. identified the promoter of human CBR3. Liver samples from black donors showed higher relative CBR3 mRNA levels than samples from whites PMID: 19590938
  17. carbonyl reductase has an important function in modifying the metastatic behavior of malignant tumors. PMID: 10728668
  18. Decreased Carbonyl Reductase expression in epithelial ovarian cancer is associated with RLN metastasis and poor survival PMID: 11592776

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Subcellular Location
Cytoplasm.
Protein Families
Short-chain dehydrogenases/reductases (SDR) family
Tissue Specificity
Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.
Database Links

HGNC: 1549

OMIM: 603608

KEGG: hsa:874

STRING: 9606.ENSP00000290354

UniGene: Hs.154510

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