Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Catechol O methyltransferase; Catechol O-methyltransferase; COMT; COMT_HUMAN; EC 2.1.1.6
Species
Homo sapiens (Human)
Expression Region
52-271aa
Target Protein Sequence
GDTKEQRILNHVLQHAEPGNAQSVLEAIDTYCEQKEWAMNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGVKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKGPGSEAGP
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Human COMT was expressed with the amino acid range of 52-271. This COMT protein is theoretically predicted to have a molecular weight of 28.3 kDa. This protein is generated in a e.coli-based system. The COMT gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant COMT protein during the following stages.
The human catechol O-methyltransferase (COMT) is a crucial enzyme involved in the metabolism of catecholamines, including dopamine, norepinephrine, and epinephrine. Its primary function is the methylation of catechol groups, playing a vital role in regulating neurotransmitter levels in the brain. The COMT gene exhibits polymorphisms, influencing enzyme activity and impacting cognitive and emotional processes. Research on COMT extends to neuropsychiatric disorders such as schizophrenia, bipolar disorder, and mood disorders, exploring associations between genetic variations and susceptibility to these conditions. Additionally, COMT inhibitors are investigated as potential therapeutics, contributing to advancements in neuropharmacology and personalized medicine. Understanding the multifaceted functions of COMT provides valuable insights into neurotransmitter regulation and its implications for mental health and therapeutic interventions.