Recombinant Human Collagen alpha-1 (XII) chain (COL12A1), partial

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Code CSB-EP859521HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
COL12A1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
COL12A1; COL12A1LCollagen alpha-1(XII) chain
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
140-316aa
Target Protein Sequence
DLVFLVDGSWSVGRNNFKYILDFIAALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYYQRDELLAAIKKIPYKGGNTMTGDAIDYLVKNTFTESAGARVGFPKVAIIITDGKSQDEVEIPARELRNVGVEVFSLGIKAADAKELKQIASTPSLNHVFNVANFDAIVDIQNEIISQV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
23.5kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
Gene References into Functions
  1. The study indicates that the spectrum of causative genes in extracellular matrix-related myopathies be extended to include COL12A1. PMID: 24334769
  2. collagen alpha-1 (XII) chain was expressed at dramatically higher (~10-fold) levels in breast PMID: 24262153
  3. Type XII collagen is overexpressed in permanent human and mouse corneal scars and could represent a new target to treat corneal scarring. PMID: 22969073
  4. In addition to binding collagen I, COMP binds to collagens XII and XIV via their C-terminal collagenous domains. PMID: 22573329
  5. COL12A1 is associated with anterior cruciate ligament ruptures among females PMID: 19443461
  6. this study identifies COL12A1 as the likely gene candidate within the recurrent 6q13 breakpoint and provides an alternative approach for detecting 6q13 anomalies in nondividing cells of chondromyxoid fibroma. PMID: 19648885
  7. induction of expression by sheare stress is associated with atherogenesis PMID: 12890494
  8. Results suggest that collagens XII and XIV might serve different functions during human embryonic development although their structures are highly similar. PMID: 15609093

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Involvement in disease
Ullrich congenital muscular dystrophy 2 (UCMD2); Bethlem myopathy 2 (BTHLM2)
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Fibril-associated collagens with interrupted helices (FACIT) family
Tissue Specificity
Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, ki
Database Links

HGNC: 2188

OMIM: 120320

KEGG: hsa:1303

STRING: 9606.ENSP00000325146

UniGene: Hs.101302

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