Recombinant Human Cyclin-K (CCNK)

In Stock
Code CSB-BP004828HU(F2)
MSDS
Size $400
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
CCNK
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
CCNK; CCNK_HUMAN; CPR 4; CPR4; Cyclin K; Cyclin-K; CyclinK; MGC9113
Species
Homo sapiens (Human)
Source
Baculovirus
Expression Region
1-354aa
Target Protein Sequence
MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPLILLQGWACRQPATHLLPSPLEDSLLCPRPFPHPACLQLGGWGGQPG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
42.9kDa
Protein Length
Full Length of isoform 2
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).
Gene References into Functions
  1. Cyclin K expression positively correlates with cell proliferation; knockdown of cyclin K or its cognate kinase CDK12 prevents the assembly of prereplicative complex in the G1 cell cycle phase. PMID: 29760377
  2. Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation PMID: 29760377
  3. Structures of CDK12/CycK complexes solved in the presence of AMP-PNP. PMID: 26597175
  4. Data show that most mutations prevent formation of the cyclin-dependent kinase 12 (Cdk12)/cyclin K (CycK) complex, rendering the kinase inactive. PMID: 25712099
  5. that cyclin K may be a novel molecular link between germ cell development, cancer development and embryonic stem cell maintenance. PMID: 25004108
  6. Cyclin K is highly expressed in mammalian testes in a developmentally regulated manner. PMID: 25004108
  7. Cyclin K interacts with CDK12 and CDK13 but not CDK9 in cells, and is required to maintain self-renewal in ES cells. PMID: 22547058
  8. Cyclin K1 is the primary cyclin partner for CDK12/CrkRS and it is required for activation of CDK12/CrkRS to phosphorylate the C-terminal domain of RNA Pol II. PMID: 22988298
  9. Cyclin K inhibits HIV-1 gene expression and replication by interfering with cyclin-dependent kinase 9 (CDK9)-cyclin T1 interaction in Nef-dependent manner. PMID: 21555514
  10. These results reveal an unexpectedly direct role for CDK9-cyclin K in checkpoint pathways that maintain genome integrity in response to replication stress. PMID: 20930849
  11. Cyclin K-expressing multiple myeloma LP-1 cells have lost their migration properties and display enhanced clonogenic capacities PMID: 20459741
  12. P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. PMID: 11884399
  13. cyclin K may play a role in regulating the cell cycle or apoptosis PMID: 11988847
  14. The cyclin K fold comprises two typical cyclin boxes with two short helices preceding the N-terminal box. A prominent feature of cyclin K is an additional helix (H4a) in the first cyclin box that obstructs the binding pocket for the p27. PMID: 17169370

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Subcellular Location
Nucleus.
Protein Families
Cyclin family, Cyclin C subfamily
Tissue Specificity
Widely expressed. Highest levels in testis.
Database Links

HGNC: 1596

OMIM: 603544

KEGG: hsa:8812

STRING: 9606.ENSP00000374529

UniGene: Hs.510409

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