Recombinant Human Cyclin-O (CCNO)

Code CSB-YP004831HU
MSDS
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Source Yeast
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Code CSB-EP004831HU
MSDS
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Source E.coli
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Code CSB-EP004831HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004831HU
MSDS
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Source Baculovirus
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Code CSB-MP004831HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CCNO
Uniprot No.
Alternative Names
CCNO; CCNO_HUMAN; CCNU; Cyclin domain containing; Cyclin like uracil DNA glycosylase; Cyclin O; Cyclin U; Cyclin-O; DGU; EC 3.2.2.; UDG2; UNG2; Uracil DNA glycolylase 2; Uracil DNA glycosylase cyclin like
Species
Homo sapiens (Human)
Expression Region
1-350
Target Protein Sequence
MVTPCPTSPS SPAARAGRRD NDQNLRAPVK KSRRPRLRRK QPLHPLNPCP LPGDSGICDL FESPSSGSDG AESPSAARGG SPLPGPAQPV AQLDLQTFRD YGQSCYAFRK AQESHFHPRE ALARQPQVTA ESRCKLLSWL IPVHRQFGLS FESLCLTVNT LDRFLTTTPV AADCFQLLGV TSLLIACKQV EVHPPRVKQL LALCCGAFSR QQLCNLECIV LHKLHFTLGA PTISFFLEHF THARVEAGQA EASEALEAQA LARGVAELSL ADYAFTSYSP SLLAICCLAL ADRMLRVSRP VDLRLGDHPE AALEDCMGKL QLLVAINSTS LTHMLPVQIC EKCSLPPSSK
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Specifically required for generation of multiciliated cells, possibly by promoting a cell cycle state compatible with centriole amplification and maturation. Acts downstream of MCIDAS to promote mother centriole amplification and maturation in preparation for apical docking.
Gene References into Functions
  1. This study presents the crystal structure of the DDB1-DCAF1-HIV-1-Vpr-uracil-DNA glycosylase (cyclin U) complex. PMID: 27571178
  2. CCNO is mutated more frequently than expected from the rare previous clinical case reports, leads to severe clinical manifestations, and should therefore be considered an important differential diagnosis of mucociliary clearance disorders. PMID: 26777464
  3. CCNO mutations have a role in congenital mucociliary clearance disorder with reduced generation of multiple motile cilia PMID: 24747639
  4. This publication corrected the mistaken attribution of uracil DNA glycosylase activity to this gene. PMID: 8419333
  5. This publication initially attributed 'uracil-DNA glycosylase' activity to this gene and named it UNG2, but a later publication (PubMed ID: 8419333) more correctly identified UNG2/CCNO as a member of the cyclin protein family. PMID: 2001396

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Involvement in disease
Ciliary dyskinesia, primary, 29 (CILD29)
Subcellular Location
Cytoplasm. Nucleus, nucleolus.
Protein Families
Cyclin family
Tissue Specificity
Present in respiratory cells (at protein level).
Database Links

HGNC: 18576

OMIM: 607752

KEGG: hsa:10309

STRING: 9606.ENSP00000282572

UniGene: Hs.3041

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