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Recombinant Human Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1)

Recombinant Human Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1)

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Code	CSB-BP007293HU
Abbreviation	Recombinant Human DYNC1I1 protein
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

DYNC1I1

Uniprot No.

O14576

Research Area

Signal Transduction

Alternative Names

Cytoplasmic dynein intermediate chain 1 Dynein intermediate chain 1, cytosolic

Species

Homo sapiens (Human)

Source

Baculovirus

Expression Region

1-645aa

Target Protein Sequence

MSDKSDLKAELERKKQRLAQIREEKKRKEEERKKKEADMQQKKEPVQDDSDLDRKRRETEALLQSIGISPEPPLVQPLHFLTWDTCYFHYLVPTPMSPSSKSVSTPSEAGSQDSGDLGPLTRTLQWDTDPSVLQLQSDSELGRRLHKLGVSKVTQVDFLPREVVSYSKETQTPLATHQSEEDEEDEEMVESKVGQDSELENQDKKQEVKEAPPRELTEEEKQQILHSEEFLIFFDRTIRVIERALAEDSDIFFDYSGRELEEKDGDVQAGANLSFNRQFYDEHWSKHRVVTCMDWSLQYPELMVASYNNNEDAPHEPDGVALVWNMKFKKTTPEYVFHCQSSVMSVCFARFHPNLVVGGTYSGQIVLWDNRSHRRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLITVSTDGKMCSWSLDMLSTPQESMELVYNKSKPVAVTGMAFPTGDVNNFVVGSEEGTVYTACRHGSKAGIGEVFEGHQGPVTGINCHMAVGPIDFSHLFVTSSFDWTVKLWTTKHNKPLYSFEDNADYVYDVMWSPVHPALFACVDGMGRLDLWNLNNDTEVPTASVAIEGASALNRVRWAQAGKEVAVGDSEGRIWVYDVGELAVPHNDEWTRFARTLVEIRANRADSEEEGTVELSA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

75.4 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In baculovirus cells, the generation of the recombinant Human DYNC1I1 protein includes integrating a DNA fragment encoding the Human DYNC1I1 protein (1-645aa) into a plasmid vector and transforming this vector into baculovirus cells. Cells containing the plasmid are screened, cultured, and induced to yield the DYNC1I1 protein. A N-terminal 10xHis tag is attached to the protein. Lysis of the cells facilitates the collection of the recombinant Human DYNC1I1 protein, which is subjected to affinity purification and then SDS-PAGE analysis and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the obtained recombinant Human DYNC1I1 protein is greater than 85%.

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Target Background

Function

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCTN1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.

Gene References into Functions

SQSTM1 is required for proper dynein motility and trafficking along microtubules. PMID: 25015291
Deletions of the exons with regulatory potential of DYNC1I1 are an example of the emerging role of exonic enhancer elements and their implications in congenital malformation syndromes PMID: 25231166
NCAM and dynein have roles in tethering dynamic microtubules and maintaining synaptic density in cortical neurons PMID: 23960070
Both P-TEFb recruitment to the HIV long terminal repeat and enhanced HIV processivity were blocked by ERK inhibitor, but not by AKT and PI3K inhibitors. PMID: 21763495
the mRNA expression level of DYNC1I1 were significantly up-regulated in tumorous liver tissues compared with corresponding nontumorous counterparts. PMID: 21767414
NDE1 and NDEL1 act upstream of LIS1 in dynein recruitment, and/or activation, on the membrane. PMID: 20048338
identification of a novel 24-kDa protein that binds directly to dynein intermediate chain [PLAC-24] PMID: 12006665