Recombinant Human Diacylglycerol kinase delta (DGKD), partial

Code CSB-YP624117HU
MSDS
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Source Yeast
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Code CSB-EP624117HU
MSDS
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Source E.coli
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Code CSB-EP624117HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP624117HU
MSDS
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Source Baculovirus
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Code CSB-MP624117HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DGKD
Uniprot No.
Alternative Names
DGKD; KIAA0145Diacylglycerol kinase delta; DAG kinase delta; EC 2.7.1.107; 130 kDa diacylglycerol kinase; Diglyceride kinase delta; DGK-delta
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). By controlling the levels of diacylglycerol, regulates for instance the PKC and EGF receptor signaling pathways and plays a crucial role during development. May also regulate clathrin-dependent endocytosis.
Gene References into Functions
  1. purification and characterization of DGKepsilon's enzymatic and conformational properties PMID: 28199087
  2. Diacylglycerol signaling limits Akt activation through diacylglycerol kinase delta and PHLPP2. PMID: 23184957
  3. This study demonistrated that the lack of association of DGKD SNPs with PD in the Han Chinese population. PMID: 22406787
  4. Zinc site mutations impair DGKdelta localization to cytoplasmic puncta and enhance localization the plasma membrane. PMID: 20857926
  5. Data indicate a novel mechanism where diacylglycerol kinase delta and protein kinase Calpha modulate the levels of ubiquitinated epidermal growth factor receptors through Akt and ubiquitin-specific protease 8. PMID: 20064931
  6. Alternative splicing of the gene generates two isoforms differing in their expression patterns and in regulatory functions PMID: 12200442
  7. Phosphorylation is involved in the control of subcellular localization of DGKdelta1. PMID: 15228384
  8. Disruption of diacylglycerol kinase delta (DGKD) is associated with seizures in humans and mice. PMID: 17357084
  9. Study identified reduced diacylglycerol kinase delta (DGKdelta) expression and DGK activity in skeletal muscle from type 2 diabetic patients. PMID: 18267070
  10. Polymerization of DGK delta regulates the activity of the enzyme by sequestering DGK delta in an inactive cellular location. PMID: 18334213

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Subcellular Location
Membrane, clathrin-coated pit. Cytoplasm.; [Isoform 1]: Cell membrane; Peripheral membrane protein. Cytoplasm.
Protein Families
Eukaryotic diacylglycerol kinase family
Tissue Specificity
[Isoform 2]: Widely expressed.; [Isoform 1]: Only detected in ovary, and to a lesser extent in spleen.
Database Links

HGNC: 2851

OMIM: 601826

KEGG: hsa:8527

STRING: 9606.ENSP00000264057

UniGene: Hs.471675

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