Recombinant Human Dual specificity protein phosphatase 16 (DUSP16)

Code CSB-YP887158HU
MSDS
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Source Yeast
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Code CSB-EP887158HU
MSDS
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Source E.coli
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Code CSB-EP887158HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887158HU
MSDS
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Source Baculovirus
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Code CSB-MP887158HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Dual specificity phosphatase 16; Dual specificity protein phosphatase 16; DUS16_HUMAN; DUSP 16; DUSP16; KIAA1700; MAP kinase phosphatase 7; MAPK phosphatase 7; Mitogen activated protein kinase phosphatase 7; Mitogen-activated protein kinase phosphatase 7; MKP 7; MKP-7; MKP7
Species
Homo sapiens (Human)
Expression Region
1-665
Target Protein Sequence
MAHEMIGTQI VTERLVALLE SGTEKVLLID SRPFVEYNTS HILEAININC SKLMKRRLQQ DKVLITELIQ HSAKHKVDID CSQKVVVYDQ SSQDVASLSS DCFLTVLLGK LEKSFNSVHL LAGGFAEFSR CFPGLCEGKS TLVPTCISQP CLPVANIGPT RILPNLYLGC QRDVLNKELM QQNGIGYVLN ASNTCPKPDF IPESHFLRVP VNDSFCEKIL PWLDKSVDFI EKAKASNGCV LVHCLAGISR SATIAIAYIM KRMDMSLDEA YRFVKEKRPT ISPNFNFLGQ LLDYEKKIKN QTGASGPKSK LKLLHLEKPN EPVPAVSEGG QKSETPLSPP CADSATSEAA GQRPVHPASV PSVPSVQPSL LEDSPLVQAL SGLHLSADRL EDSNKLKRSF SLDIKSVSYS ASMAASLHGF SSSEDALEYY KPSTTLDGTN KLCQFSPVQE LSEQTPETSP DKEEASIPKK LQTARPSDSQ SKRLHSVRTS SSGTAQRSLL SPLHRSGSVE DNYHTSFLFG LSTSQQHLTK SAGLGLKGWH SDILAPQTST PSLTSSWYFA TESSHFYSAS AIYGGSASYS AYSCSQLPTC GDQVYSVRRR QKPSDRADSR RSWHEESPFE KQFKRRSCQM EFGESIMSEN RSREELGKVG SQSSFSGSME IIEVS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.
Gene References into Functions
  1. The crystal structure of JNK1 bound to the catalytic domain of MKP7 at 2.4-A resolution, providing high-resolution structural insight into the FXF-docking interaction, is reported. PMID: 26988444
  2. The DUSP16 ablation leads to a G1/S transition arrest, reduced incorporation of 5-bromodeoxyuridine, enhanced senescence-associated beta-galactosidase activity, and formation of senescence-associated heterochromatic foci. PMID: 26381291
  3. analysis of the interaction of the MAPK binding domain of DUSP16 with p38alpha PMID: 23926106
  4. PPARdelta-mediated messenger RNA stabilization of mitogen-activated protein kinase phosphatase (MKP)-7 was responsible for the GW501516-mediated inhibition of JNK signaling. PMID: 23639976
  5. Data indicate that the activities of phosphoprotein phosphatases MKP5 and MKP7 were determined in the system. PMID: 23233447
  6. MKP-7, a negative regulator of JNK, regulates VCAM-1 expression in activated endothelial cells through IRF-1 but not GATA6. PMID: 22182512
  7. DUSPs 10 and 16 are positive regulators of activation, apparently acting by modulating cross-talk between the p38 and ERK pathways. PMID: 22245064
  8. DUSP16 is a new epigenetically regulated determinant of JNK activation in BL. PMID: 20551953
  9. MKP-7 down-regulates ERK-dependent gene expression by blocking nuclear accumulation of phospho-ERK. PMID: 20122898
  10. context-dependent role for DUSP16 on cell transformation and apoptosis. PMID: 14586399
  11. activation of the ERK pathway strongly blocks JNK activation through stabilization of MKP-7 mediated by phosphorylation PMID: 15689616
  12. MKP7 binds beta-arrestin 2 via amino acids 394-443 of MKP7, the same region that interacts with JIP-1 PMID: 15888437
  13. identification of an intricate SDF-1alpha-induced signaling cascade that involves eNOS, JNK3, and MKP7and enhances endothelial migration PMID: 19307591

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Subcellular Location
Cytoplasm. Nucleus. Cytoplasmic vesicle. Note=After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Database Links

HGNC: 17909

OMIM: 607175

KEGG: hsa:80824

STRING: 9606.ENSP00000228862

UniGene: Hs.536535

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