Recombinant Human Dual specificity protein phosphatase 2 (DUSP2)

Code CSB-YP2049HU
MSDS
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Source Yeast
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Code CSB-EP2049HU
MSDS
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Source E.coli
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Code CSB-EP2049HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP2049HU
MSDS
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Source Baculovirus
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Code CSB-MP2049HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Dual specificity protein phosphatase 2; EC 3.1.3.16; EC 3.1.3.48; Dual specificity protein phosphatase PAC-1; DUSP2 PAC1
Species
Homo sapiens (Human)
Expression Region
1-314
Target Protein Sequence
MGLEAARELECAALGTLLRDPREAERTLLLDCRPFLAFCRRHVRAARPVPWNALLRRRARGPPAAVLACLLPDRALRTRLVRGELARAVVLDEGSASVAELRPDSPAHVLLAALLHETRAGPTAVYFLRGGFDGFQGCCPDLCSEAPAPALPPTGDKTSRSDSRAPVYDQGGPVEILPYLFLGSCSHSSDLQGLQACGITAVLNVSASCPNHFEGLFRYKSIPVEDNQMVEISAWFQEAIGFIDWVKNSGGRVLVHCQAGISRSATICLAYLMQSRRVRLDEAFDFVKQRRGVISPNFSFMGQLLQFETQVLCH
Protein Length
Full Length
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
Gene References into Functions
  1. Regulation of atypical MAP kinases ERK3 and ERK4 by the phosphatase DUSP2 has been reported. PMID: 28252035
  2. hypoxia-induced IL-6 production in endometriotic lesions is mediated via downregulation of DUSP2, which causes aberrant activation of STAT3 signaling pathway and helps the endometriotic cells survive under the ectopic environment PMID: 28440564
  3. Authors report that hypoxia-mediated downregulation of the dual specificity phosphatase 2 (DUSP2) is critical for the accumulation of CSC in colorectal cancer. PMID: 28652251
  4. Hypoxia inhibits DUSP2 expression in colon cancer, leading to up-regulation of IL-8, which facilitates angiogenesis and tumour metastasis. PMID: 28026024
  5. Our data show that aberrant epigenetic inactivation of DUSP2 occurs in carcinogenesis and that CTCF is involved in the epigenetic regulation of DUSP2 expression. PMID: 26833217
  6. Highly recurrent mutation of DUSP2 is associated with nodular lymphocyte predominant Hodgkin lymphoma. PMID: 26658840
  7. identified among the key genes in circulating monocytes that were altered by exercise PMID: 26207425
  8. Data show that hypoxia promotes lapatinib resistance in ERBB2-positive breast cancer cells through activation of the MEK-ERK pathway a HIF-1-dependent manner via regulation of dual-specificity phosphatase 2 (DUSP2). PMID: 25596742
  9. DUSP2 is an important molecule in endometrial physiology and that hypoxia-inhibited DUSP2 expression is a critical factor for the development of endometriosis. PMID: 21984126
  10. Dipyridamole reduced expression of PAC-1 and CD62p in patients with malignant lymphoma. PMID: 20723301
  11. DUSP2 is a key downstream regulator of HIF-1-mediated tumor progression and chemoresistance PMID: 21490398
  12. During apoptosis, p53 activates transcription of PAC1 by binding to a palindromic site in the PAC1 promoter PMID: 12673251
  13. relationships between the expression levels of CD61, CD63, and PAC-1 on the platelet surface and the incidences of acute rejection and tubular necrosis as well as the recovery of graft function after renal transplantation PMID: 12826159
  14. variation in large granular lymphocyte leukemia PMID: 14680939
  15. These results reveal a crucial role of PAC1 in E2F-1-directed apoptosis. PMID: 17471234
  16. Induction of hypercortisolism in healthy volunteers was not associated with changes in the platelet activation marker PAC-1 or the number of circulating platelet-leucocyte aggregates. PMID: 18600034

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Subcellular Location
Nucleus.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Tissue Specificity
Expressed in hematopoietic tissues.
Database Links

HGNC: 3068

OMIM: 603068

KEGG: hsa:1844

STRING: 9606.ENSP00000288943

UniGene: Hs.1183

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