Recombinant Human EF-hand domain-containing family member A1 (EFHA1)

Code CSB-YP818265HU
MSDS
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Source Yeast
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Code CSB-EP818265HU
MSDS
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Source E.coli
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Code CSB-EP818265HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818265HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP818265HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MICU2
Uniprot No.
Alternative Names
1110008L20Rik; EF hand domain containing family member A1; EF hand domain family A1; EF hand domain family member A1; EF-hand domain-containing family member A1; EFHA1; EFHA1 EF hand domain family member A1; EFHA1_HUMAN; FLJ25016; FLJ34588; Smhs2 homolog
Species
Homo sapiens (Human)
Expression Region
1-434
Target Protein Sequence
MAAAAGSCAR VAAWGGKLRR GLAVSRQAVR SPGPLAAAVA GAALAGAGAA WHHSRVSVAA RDGSFTVSAQ KNVEHGIIYI GKPSLRKQRF MQFSSLEHEG EYYMTPRDFL FSVMFEQMER KTSVKKLTKK DIEDTLSGIQ TAGCGSTFFR DLGDKGLISY TEYLFLLTIL TKPHSGFHVA FKMLDTDGNE MIEKREFFKL QKIISKQDDL MTVKTNETGY QEAIVKEPEI NTTLQMRFFG KRGQRKLHYK EFRRFMENLQ TEIQEMEFLQ FSKGLSFMRK EDFAEWLLFF TNTENKDIYW KNVREKLSAG ESISLDEFKS FCHFTTHLED FAIAMQMFSL AHRPVRLAEF KRAVKVATGQ ELSNNILDTV FKIFDLDGDE CLSHEEFLGV LKNRMHRGLW VPQHQSIQEY WKCVKKESIK GVKEVWKQAG KGLF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low. MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium. MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting calcium, avoiding energy dissipation and cell-death triggering.
Gene References into Functions
  1. MICU2 restricts spatial crosstalk between InsP3R and MCU channels by regulating threshold and gain of MICU1-mediated inhibition and activation of MCU. PMID: 29241542
  2. MICU2 expression in the heart tissues from patients with ventricular hypertrophy. PMID: 29073106
  3. study concludes that cooperative, high-affinity interaction of the MICU1-MICU2 complex with Ca(2+) serves as an on-off switch, leading to a tightly controlled channel, capable of responding directly to cytosolic Ca(2+) signals PMID: 28615291
  4. This is the first demonstration of MICU2 deficiency in humans, which we suggest causes a distinct neurodevelopmental phenotype secondary to impaired mitochondrial calcium uniporter-mediated regulation of intracellular calcium homeostasis. PMID: 29053821
  5. Expression of MICU2 mutants lacking functional Ca2+-binding sites leads to a striking loss of Ca2+ uptake in HEK293 cells. PMID: 24503055
  6. MICU1 and MICU2 have roles in tuning the mitochondrial Ca2+ uniporter by exerting opposite effects on MCU activity PMID: 24560927
  7. The results identify MICU2 as a new component of the uniporter complex that may contribute to the tissue-specific regulation of this channel. PMID: 23409044
  8. regulation of MCU-mediated mitochondrial calcium handling PMID: 23409044
  9. Observational study of gene-disease association. (HuGE Navigator) PMID: 20877624
  10. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614

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Subcellular Location
Mitochondrion intermembrane space.
Protein Families
MICU1 family, MICU2 subfamily
Database Links

HGNC: 31830

OMIM: 610632

KEGG: hsa:221154

STRING: 9606.ENSP00000371811

UniGene: Hs.412103

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