Recombinant Human Ephrin-B3 (EFNB3), partial

Code CSB-YP007467HU
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Source Yeast
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Code CSB-EP007467HU
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Source E.coli
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Code CSB-EP007467HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007467HU
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Source Baculovirus
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Code CSB-MP007467HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EFNB3
Uniprot No.
Alternative Names
EFL 6; EFL6; EFNB 3; Efnb3; EFNB3_HUMAN; EPH related receptor transmembrane ligand ELK L3; EPH related receptor transmembrane ligand ELKL3; EPH related receptor tyrosine kinase ligand 8; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 8; Ephrin B3; Ephrin-B3; EphrinB3; EPLG 8; EPLG8; LERK 8; LERK-8; LERK8
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.; (Microbial infection) Acts as a receptor for nipah virus and hendra virus.
Gene References into Functions
  1. The study provides evidence from single nucleotide polymorphism analyses of ADVANCE study demonstrating that EFNB3 is a hypertension risk gene. PMID: 28272517
  2. Data suggest that molecules in the erythropoietin-producing hepatocellular receptor B family (EPHB) / ephrinB (EFNB) signalling pathways, specifically ephrin B3 and GRIP1, are involved blood pressure regulation. PMID: 27941904
  3. Data show that Ephrin B3 was concomitantly expressed with EphA2 and Ephrin A1 with higher Ephrin B3 levels found in non-squamous than in squamous tumors. PMID: 27533087
  4. Study found up-regulated expression of ephrinB3/EphB3 in intractable temporal lobe epilepsy patients and experimental temporal lobe epilepsy rats, which suggested that ephrinB3/EphB3 might be involved in the pathogenesis of temporal lobe epilepsy PMID: 26930615
  5. Findings suggest that EphrinB3 may be a relevant target for promoting remyelination in demyelinating disease PMID: 26687980
  6. Ephrin-B3 binds to B lymphocytes, most likely via a non-classical receptor, and induces migration of the memory B cell subpopulation. PMID: 21447033
  7. Phosphoproteomic profiling of nonsmall cell lung cancer cells reveals that ephrin B3 regulates pro-survival signaling through Akt1-mediated phosphorylation of the EphA2 receptor. PMID: 21413766
  8. evidence for an unknown ephrin-B3-binding cell-surface proteoglycan involved in cellular signalling PMID: 20925654
  9. EphrinB3 is a bona fide alternate receptor for NiV entry, and two residues in the G-H loop of the ephrin B-class ligands are critical determinants of NiV receptor activity. PMID: 16477309
  10. Immunohistochemistry shows robust staining for phosphorylated ephrin-B and ephrin-B3 in invading glioblastoma cells. PMID: 16951161
  11. Transgenic EphB1 and ephrin-B3 cooperatively regulate the proliferation and migration of neural progenitors in the hippocampus PMID: 18057206
  12. report the crystal structures of the NiV-G both in its receptor-unbound state and in complex with ephrin-B3, providing, to our knowledge, the first view of a paramyxovirus attachment complex in which a cellular protein is used as the virus receptor PMID: 18632560

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Ephrin family
Tissue Specificity
Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Database Links

HGNC: 3228

OMIM: 602297

KEGG: hsa:1949

STRING: 9606.ENSP00000226091

UniGene: Hs.26988

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