Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cell Biology
Alternative Names
DKFZP564P0622; F box and leucine rich repeat protein 2; F box protein containing leucine rich repeats; F box protein FBL2/FBL3; F box/LRR repeat protein 2; F-box and leucine-rich repeat protein 2; F-box protein FBL2/FBL3; F-box/LRR-repeat protein 2; FBL 2; FBL 3; FBL2; FBL3; FBXL 2; FBXL2; FBXL2_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-423aa
Target Protein Sequence
MVFSNNDEGLINKKLPKELLLRIFSFLDIVTLCRCAQISKAWNILALDGSNWQRIDLFNFQTDVEGRVVENISKRCGGFLRKLSLRGCIGVGDSSLKTFAQNCRNIEHLNLNGCTKITDSTCYSLSRFCSKLKHLDLTSCVSITNSSLKGISEGCRNLEYLNLSWCDQITKDGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNYCHELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEAARCSHLTDAGFTLLARNCHELEKMDLEECILITDSTLIQLSIHCPKLQALSLSHCELITDDGILHLSNSTCGHERLRVLELDNCLLITDVALEHLENCRGLERLELYDCQQVTRAGIKRMRAQLPHVKVHAYFAPVTPPTAVAGSGQRLCRCCVIL
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-423 constitute the expression domain of recombinant Human FBXL2. This FBXL2 protein is expected to have a theoretical molecular weight of 63.1 kDa. This FBXL2 recombinant protein is manufactured in e.coli. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of FBXL2, making it easier to detect and purify the FBXL2 recombinant protein in the later stages of expression and purification.
The human F-box/LRR-repeat protein 2 (FBXL2) is a member of the F-box protein family, serving as a substrate recognition component of the SCF (Skp1-Cul1-F-box protein) ubiquitin ligase complex. FBXL2 contains leucine-rich repeat (LRR) motifs, facilitating its interaction with specific substrates for ubiquitination and subsequent degradation by the proteasome. FBXL2 is involved in the regulation of diverse cellular processes, including cell cycle progression, signal transduction, and apoptosis. Its substrates include key proteins such as β-catenin and SREBP (sterol regulatory element-binding protein). Research on FBXL2 aims to elucidate its roles in cellular homeostasis, and development, and its potential implications in various diseases, including cancer and metabolic disorders.
