Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

FBXL2

Uniprot No.

Q9UKC9

Research Area

Cell Biology

Alternative Names

DKFZP564P0622; F box and leucine rich repeat protein 2; F box protein containing leucine rich repeats; F box protein FBL2/FBL3; F box/LRR repeat protein 2; F-box and leucine-rich repeat protein 2; F-box protein FBL2/FBL3; F-box/LRR-repeat protein 2; FBL 2; FBL 3; FBL2; FBL3; FBXL 2; FBXL2; FBXL2_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-423aa

Target Protein Sequence

MVFSNNDEGLINKKLPKELLLRIFSFLDIVTLCRCAQISKAWNILALDGSNWQRIDLFNFQTDVEGRVVENISKRCGGFLRKLSLRGCIGVGDSSLKTFAQNCRNIEHLNLNGCTKITDSTCYSLSRFCSKLKHLDLTSCVSITNSSLKGISEGCRNLEYLNLSWCDQITKDGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNYCHELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEAARCSHLTDAGFTLLARNCHELEKMDLEECILITDSTLIQLSIHCPKLQALSLSHCELITDDGILHLSNSTCGHERLRVLELDNCLLITDVALEHLENCRGLERLELYDCQQVTRAGIKRMRAQLPHVKVHAYFAPVTPPTAVAGSGQRLCRCCVIL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

63.1kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-423 constitute the expression domain of recombinant Human FBXL2. This FBXL2 protein is expected to have a theoretical molecular weight of 63.1 kDa. This FBXL2 recombinant protein is manufactured in e.coli. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of FBXL2, making it easier to detect and purify the FBXL2 recombinant protein in the later stages of expression and purification.

The human F-box/LRR-repeat protein 2 (FBXL2) is a member of the F-box protein family, serving as a substrate recognition component of the SCF (Skp1-Cul1-F-box protein) ubiquitin ligase complex. FBXL2 contains leucine-rich repeat (LRR) motifs, facilitating its interaction with specific substrates for ubiquitination and subsequent degradation by the proteasome. FBXL2 is involved in the regulation of diverse cellular processes, including cell cycle progression, signal transduction, and apoptosis. Its substrates include key proteins such as β-catenin and SREBP (sterol regulatory element-binding protein). Research on FBXL2 aims to elucidate its roles in cellular homeostasis, and development, and its potential implications in various diseases, including cancer and metabolic disorders.

Target Background

Function

Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy. PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant.

Gene References into Functions

PTEN counteracts FBXL2 to promote IP3R3- and Ca(2+)-mediated apoptosis limiting tumour growth PMID: 28614300
these results uncover a previous unknown network involving FBXL2 and FoxM1 in the regulation of gastric cancer growth. PMID: 26790640
FBXL2 recognizes Trp-73 within NALP3 for interaction and targets Lys-689 within NALP3 for ubiquitin ligation and degradation. PMID: 26037928
FBXL2 mediates the ubiquitylation and degradation of p85beta on cell membranes. PMID: 23604317
These results suggested that the involvement of the reduction of FBL2 level is related to AD progression. PMID: 22455980
FBXL2 is indispensible regulator of mitosis that serves as a tumor suppressor PMID: 22020328
FBXL2 targets cyclin D2 for ubiquitination and degradation to inhibit leukemic cell proliferation. PMID: 22323446
FBL2 is a novel and dual regulator of amyloid precursor protein (APP) metabolism through FBL2-dependent ubiquitination of APP. PMID: 22399757

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Subcellular Location

Membrane; Lipid-anchor.

Tissue Specificity

Expressed in brain, heart, kidney, liver, lung, pancreas and placenta.

Database Links

HGNC: 13598

OMIM: 605652

KEGG: hsa:25827

STRING: 9606.ENSP00000417601

UniGene: Hs.475872

Code	CSB-EP892134HU
Abbreviation	Recombinant Human FBXL2 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human F-box/LRR-repeat protein 2 (FBXL2)

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