Purity

≥ 95% as determined by SDS-PAGE.
≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human FGFR2 at 2 μg/mL can bind Anti-FGFR2 recombinant antibody (CSB-RA008645MA1HU). The EC50 is 1.066-1.203 ng/mL.

Target Names

FGFR2

Uniprot No.

P21802-3

Alternative Names

Fibroblast growth factor receptor 2; FGFR-2; EC 2.7.10.1; K-sam; KGFR; Keratinocyte growth factor receptor; CD antigen CD332

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

22-378aa

Target Protein Sequence

RPSFSLVEDTTLEPEEPPTKYQISQPEVYVAAPGESLEVRCLLKDAAVISWTKDGVHLGPNNRTVLIGEYLQIKGATPRDSGLYACTASRTVDSETWYFMVNVTDAISSGDDEDDTDGAEDFVSENSNNKRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCVVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTEADAGEYICKVSNYIGQANQSAWLTVLPKQQAPGREKEITASPDYLE

Mol. Weight

41.0 kDa

Protein Length

Partial of Isoform 3

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

FGFR2 dysregulation drives oncogenesis in gastric, breast, and endometrial cancers, making this receptor a critical target for therapeutic antibody development and small-molecule inhibitor screening. This mammalian-expressed construct spanning residues 22–378 of isoform 3 captures the complete extracellular ligand-binding domain, ensuring native glycosylation patterns that preserve conformational epitopes essential for antibody epitope mapping and blocking antibody screening. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 1.066–1.203 ng/mL, confirming that the protein retains its native structure and supports quantitative ligand-receptor interaction assays, competitive inhibition studies, and affinity characterization by surface plasmon resonance or biolayer interferometry. Dual chromatographic analysis confirms >95% purity by both SDS-PAGE and SEC-HPLC, and endotoxin levels below 1.0 EU/μg satisfy the contamination thresholds typical for cell-based assays in drug discovery workflows.

Code	CSB-MP008645HU(F3)
Abbreviation	Recombinant Human FGFR2 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human FGFR2 at 2 μg/ml can bind Anti-FGFR2 recombinant antibody (CSB-RA008645MA1HU). The EC₅₀ is 1.066-1.203 ng/mL. Biological Activity Assay The purity of FGFR2 was greater than 95% as determined by SEC-HPLC
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Recombinant Human Fibroblast growth factor receptor 2 (FGFR2), partial (Active)

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