Recombinant Human Forkhead box protein D3(FOXD3)

Code CSB-YP890729HU
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Source Yeast
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Code CSB-EP890729HU
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Source E.coli
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Code CSB-EP890729HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890729HU
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Source Baculovirus
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Code CSB-MP890729HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names FOXD3
Uniprot No. Q9UJU5
Alternative Names AIS1; forkhead box D3; Forkhead box protein D3; foxd3; FOXD3_HUMAN; Genesis; HFH2; HNF3/FH transcription factor genesis; VAMAS2
Species Homo sapiens (Human)
Expression Region 1-478
Target Protein Sequence MTLSGGGSAS DMSGQTVLTA EDVDIDVVGE GDDGLEEKDS DAGCDSPAGP PELRLDEADE VPPAAPHHGQ PQPPHQQPLT LPKEAAGAGA GPGGDVGAPE ADGCKGGVGG EEGGASGGGP GAGSGSAGGL APSKPKNSLV KPPYSYIALI TMAILQSPQK KLTLSGICEF ISNRFPYYRE KFPAWQNSIR HNLSLNDCFV KIPREPGNPG KGNYWTLDPQ SEDMFDNGSF LRRRKRFKRH QQEHLREQTA LMMQSFGAYS LAAAAGAAGP YGRPYGLHPA AAAGAYSHPA AAAAAAAAAA LQYPYALPPV APVLPPAVPL LPSGELGRKA AAFGSQLGPG LQLQLNSLGA AAAAAGTAGA AGTTASLIKS EPSARPSFSI ENIIGGGPAA PGGSAVGAGV AGGTGGSGGG STAQSFLRPP GTVQSAALMA THQPLSLSRT TATIAPILSV PLSGQFLQPA ASAAAAAAAA AQAKWPAQ
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
Gene References into Functions
  1. FOXD3 acts as a repressor of the mitochondrial S-adenosylmethionine carrier (SLC25A26) gene expression in cancer cells. PMID: 30076902
  2. On the basis of these data, FOXD3 is a potent repressor of DCLK1-S expression in normal cells; loss of FOXD3 in hCCCs/hCRCs allows upregulation of DCLK1-S, imparting a potent invasive potential to the cells PMID: 28851816
  3. FOXD3 knockdown resulted in enhanced ATC proliferation, invasion and migration and diminished cellular apoptosis. Further, we showed that FOXD3 regulated expression of E-cadherin by modulating MAPK/EKR signaling pathway that promotes EMT and metastasis during thyroid carcinogenesis. PMID: 28430585
  4. Results show that FOXD3 expression was reduced in colon cancer cells. Its knockdown dramatically increased the proliferation of tumor cells, enhanced cell invasiveness and inhibited cell apoptosis. The study indicates that FOXD3 may play a protective role in human colon formation by regulating EGFR/Ras/Raf/MEK/ERK signal pathway. PMID: 27926503
  5. FOXO4 and FOXD3 were shown independently predictive of overall survival in gastric cancer PMID: 27027443
  6. FOXD3/miR-214/MED19 axis is important for the regulation of growth, invasion and metastasis of colorectal cancer PMID: 27811858
  7. total of 1799 differentially methylated regions were identified including SLC6A3, Rab40C, ZNF584, and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction.Methylation of those aforementioned genes in white blood cells of our young patients may highlight their potential as early epimarkers PMID: 28349825
  8. Results showed that silencing FoxD3 in lung cancer cell lines stimulated cell growth and inhibited cell apoptosis. PMID: 25894280
  9. FOXD3 is sufficient but not necessary to drive PAX3 expression in melanoma cells. PMID: 26252164
  10. The present study finds that the aspirin-FOXD3-OLA1P2-STAT3 axis exhibits exciting anticancer effects and provides new insights into the chemopreventive mechanisms underlying aspirin use PMID: 26898989
  11. FOXD3 might serve as an independent prognostic biomarker and a potential therapeutic target for high-grade gliomas, which warrant further investigation. PMID: 26011451
  12. Down-regulation of FOXD3 is associated with metastasis in hepatocellular carcinoma. PMID: 26112097
  13. Data show that transcription factors PAX3 and FOXD3-mediated melanoma cell migration is dependent on promoting the expression of chemokine receptor CXCR4. PMID: 26205821
  14. In addition to a possible association of rs78645479 in FoxD3 with vitiligo, our data on the association of this FoxD3 variant with thyroid autoantibodies suggest a potential involvement of FoxD3 in thyroid immunoregulation. PMID: 26267147
  15. FOXD3 overexpression significantly inhibits cell growth and results in G1 cell cycle arrest in NSCLC A549 and H1299 cells. PMID: 26341266
  16. FOXD3 and TWIST1 define distinct subgroups of cells within a heterogeneous tumor. PMID: 25061102
  17. Findings indicate that miR-137 is a valuable biomarker for hepatocellular carcinoma (HCC) prognosis and the forkhead box D3 (FoxD3)/miR-137/AKT2 regulatory network plays an important role in HCC progression. PMID: 24970808
  18. Results suggest an inverse relationship between FoxD3 expression and tumor metastasis in invasive ductal carcinomas of the breast. PMID: 24551288
  19. Results indicate that FOXD3 exhibits tumor suppressive activity that affects the growth, aggressiveness and angiogenesis of NB through transcriptional regulation of NDRG1. PMID: 24269992
  20. Our results indicate FOXD3 exhibits tumor suppressive activity and may be useful for breast therapy. PMID: 24632201
  21. Foxd3 is both sufficient and necessary for regulating the balance between melanocyte and Schwann cell development. In addition, Foxd3 is also sufficient to regulate the switch between neuronal and glial fates in sensory ganglia. PMID: 23858437
  22. upregulation of ERBB3 is involved in melanoma adapting to RAF/MEK inhibitors through FOXD3 PMID: 23543055
  23. Methylation profile analyses identified the promoter of FOXD3 as the only genomic region with increased methylation in mice and humans during progression of Helicobacter pylori-associated gastric tumors. PMID: 23058321
  24. A balance of FOXD3 activity is required to maintain pluripotency and paraxial mesoderm fates. PMID: 22887036
  25. Six nonsynonymous FOXD3 variants were identified in human ocular disease. PMID: 22815627
  26. FOXD3 regulates migration properties and Rnd3 expression in melanoma cells PMID: 21478267
  27. Studies show FOXD3 is suppressed by B-RAF, uncover a novel role and mechanism for FOXD3 as a negative cell cycle regulator, and have implications for the repression of melanocytic lineage cells. PMID: 20332228
  28. candidate for vitilago susceptibility and autosomal dominant with promoter variation PMID: 16098053
  29. HIV-1 induces OTK18 expression through a YIN-YANG-1 mediated manner in human macrophages, although its gene expression is suppressed by FoxD3 upregulation PMID: 19034670

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Involvement in disease Autoimmune disease 1 (AIS1)
Subcellular Location Nucleus
Tissue Specificity Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
Database Links

HGNC: 3804

OMIM: 607836

KEGG: hsa:27022

STRING: 9606.ENSP00000360157

UniGene: Hs.546573

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