anti-Homo sapiens (Human) FOXD3 Antibody raised in Rabbit

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FOXD3 Polyclonal antibody

Uniprot No.

Target Names

FOXD3

Alternative Names

AIS1 antibody; forkhead box D3 antibody; Forkhead box protein D3 antibody; foxd3 antibody; FOXD3_HUMAN antibody; Genesis antibody; HFH2 antibody; HNF3/FH transcription factor genesis antibody; VAMAS2 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Forkhead box protein D3 protein (1-140AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function	Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
Gene References into Functions	FOXD3 acts as a repressor of the mitochondrial S-adenosylmethionine carrier (SLC25A26) gene expression in cancer cells. PMID: 30076902 On the basis of these data, FOXD3 is a potent repressor of DCLK1-S expression in normal cells; loss of FOXD3 in hCCCs/hCRCs allows upregulation of DCLK1-S, imparting a potent invasive potential to the cells PMID: 28851816 FOXD3 knockdown resulted in enhanced ATC proliferation, invasion and migration and diminished cellular apoptosis. Further, we showed that FOXD3 regulated expression of E-cadherin by modulating MAPK/EKR signaling pathway that promotes EMT and metastasis during thyroid carcinogenesis. PMID: 28430585 Results show that FOXD3 expression was reduced in colon cancer cells. Its knockdown dramatically increased the proliferation of tumor cells, enhanced cell invasiveness and inhibited cell apoptosis. The study indicates that FOXD3 may play a protective role in human colon formation by regulating EGFR/Ras/Raf/MEK/ERK signal pathway. PMID: 27926503 FOXO4 and FOXD3 were shown independently predictive of overall survival in gastric cancer PMID: 27027443 FOXD3/miR-214/MED19 axis is important for the regulation of growth, invasion and metastasis of colorectal cancer PMID: 27811858 total of 1799 differentially methylated regions were identified including SLC6A3, Rab40C, ZNF584, and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction.Methylation of those aforementioned genes in white blood cells of our young patients may highlight their potential as early epimarkers PMID: 28349825 Results showed that silencing FoxD3 in lung cancer cell lines stimulated cell growth and inhibited cell apoptosis. PMID: 25894280 FOXD3 is sufficient but not necessary to drive PAX3 expression in melanoma cells. PMID: 26252164 The present study finds that the aspirin-FOXD3-OLA1P2-STAT3 axis exhibits exciting anticancer effects and provides new insights into the chemopreventive mechanisms underlying aspirin use PMID: 26898989 FOXD3 might serve as an independent prognostic biomarker and a potential therapeutic target for high-grade gliomas, which warrant further investigation. PMID: 26011451 Down-regulation of FOXD3 is associated with metastasis in hepatocellular carcinoma. PMID: 26112097 Data show that transcription factors PAX3 and FOXD3-mediated melanoma cell migration is dependent on promoting the expression of chemokine receptor CXCR4. PMID: 26205821 In addition to a possible association of rs78645479 in FoxD3 with vitiligo, our data on the association of this FoxD3 variant with thyroid autoantibodies suggest a potential involvement of FoxD3 in thyroid immunoregulation. PMID: 26267147 FOXD3 overexpression significantly inhibits cell growth and results in G1 cell cycle arrest in NSCLC A549 and H1299 cells. PMID: 26341266 FOXD3 and TWIST1 define distinct subgroups of cells within a heterogeneous tumor. PMID: 25061102 Findings indicate that miR-137 is a valuable biomarker for hepatocellular carcinoma (HCC) prognosis and the forkhead box D3 (FoxD3)/miR-137/AKT2 regulatory network plays an important role in HCC progression. PMID: 24970808 Results suggest an inverse relationship between FoxD3 expression and tumor metastasis in invasive ductal carcinomas of the breast. PMID: 24551288 Results indicate that FOXD3 exhibits tumor suppressive activity that affects the growth, aggressiveness and angiogenesis of NB through transcriptional regulation of NDRG1. PMID: 24269992 Our results indicate FOXD3 exhibits tumor suppressive activity and may be useful for breast therapy. PMID: 24632201 Foxd3 is both sufficient and necessary for regulating the balance between melanocyte and Schwann cell development. In addition, Foxd3 is also sufficient to regulate the switch between neuronal and glial fates in sensory ganglia. PMID: 23858437 upregulation of ERBB3 is involved in melanoma adapting to RAF/MEK inhibitors through FOXD3 PMID: 23543055 Methylation profile analyses identified the promoter of FOXD3 as the only genomic region with increased methylation in mice and humans during progression of Helicobacter pylori-associated gastric tumors. PMID: 23058321 A balance of FOXD3 activity is required to maintain pluripotency and paraxial mesoderm fates. PMID: 22887036 Six nonsynonymous FOXD3 variants were identified in human ocular disease. PMID: 22815627 FOXD3 regulates migration properties and Rnd3 expression in melanoma cells PMID: 21478267 Studies show FOXD3 is suppressed by B-RAF, uncover a novel role and mechanism for FOXD3 as a negative cell cycle regulator, and have implications for the repression of melanocytic lineage cells. PMID: 20332228 candidate for vitilago susceptibility and autosomal dominant with promoter variation PMID: 16098053 HIV-1 induces OTK18 expression through a YIN-YANG-1 mediated manner in human macrophages, although its gene expression is suppressed by FoxD3 upregulation PMID: 19034670 Show More Hide All
Involvement in disease	Autoimmune disease 1 (AIS1)
Subcellular Location	Nucleus
Tissue Specificity	Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
Database Links	HGNC: 3804 OMIM: 607836 KEGG: hsa:27022 STRING: 9606.ENSP00000360157 UniGene: Hs.546573

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Check Code：

Code	CSB-PA890729LA01HU
Size	US$299
Image	Western Blot Positive WB detected in: Rat kidney tissue All lanes: FOXD3 antibody at 3.2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa Immunohistochemistry of paraffin-embedded human glioma using CSB-PA890729LA01HU at dilution of 1:100 Immunofluorescence staining of Hela cells with CSB-PA890729LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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