Recombinant Human G-protein coupled receptor-associated sorting protein 1 (GPRASP1), partial

Code CSB-YP683365HU
MSDS
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Source Yeast
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Code CSB-EP683365HU
MSDS
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Source E.coli
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Code CSB-EP683365HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP683365HU
MSDS
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Source Baculovirus
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Code CSB-MP683365HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GPRASP1
Uniprot No.
Alternative Names
G-protein coupled receptor-associated sorting protein 1; GASP; GASP-1; GASP1; GASP1_HUMAN; Gprasp1; KIAA0443
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.
Gene References into Functions
  1. Study demonstrated that binding of clozapine or olanzapine to the 5-HT7 receptor leads to antagonist-mediated lysosomal degradation by exposing key residues in the C-terminal tail that interact with GASP-1 PMID: 25706089
  2. Data suggest that specific serine/threonine residues in DRD2 (dopamine receptor D2) are involved in regulation of DRD2 endocytosis by GRK2 (G-protein coupled receptor kinase 2) and GASP1. PMID: 23082996
  3. GASP1 was identified as a potential new serum and biomarker for brain, pancreatic, lung, and breast cancers, and gliomas. PMID: 22483848
  4. LIR was able to induce gains in 1RM and quadriceps CSA similar to those observed after traditional HI. These responses may be related to the concomitant decrease in MSTN and increase in FLST isoforms, GASP-1, and SMAD-7 mRNA gene expression. PMID: 21900845
  5. These studies identify GASP-1 as a potential new serum and tumor biomarker for breast cancer. PMID: 21791203
  6. following this endocytosis the D(3) dopamine receptors fail to recycle and are instead targeted for degradation through an interaction with the G protein-coupled receptor (GPCR)-associated sorting protein-1 (GASP-1) PMID: 21030592
  7. This review describes the discovery of GASPs, their major features, interacting partners, functions and possible involvement in pathological situations including neurodegenerative diseases and cancer. PMID: 20394773
  8. identified a protein that binds preferentially to the cytoplasmic tail of the delta opioid receptor as a candidate heterotrimeric GTP-binding protein (G protein)-coupled receptor-associated sorting protein (GASP) [GASP] PMID: 12142540
  9. Modulation of the D2-GASP interaction is important for the functional down-regulation of D2 dopamine receptors. PMID: 16049099

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Subcellular Location
Cytoplasm.
Protein Families
GPRASP family
Tissue Specificity
Expressed in the brain, with lower expression in medulla, spinal cord and substantia nigra.
Database Links

HGNC: 24834

OMIM: 300417

KEGG: hsa:9737

STRING: 9606.ENSP00000355146

UniGene: Hs.729265

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