Recombinant Human Glutamate dehydrogenase 2, mitochondrial (GLUD2)

Code CSB-YP009545HU
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Source Yeast
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Code CSB-EP009545HU
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Source E.coli
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Code CSB-EP009545HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009545HU
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Source Baculovirus
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Code CSB-MP009545HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GLUD2
Uniprot No.
Alternative Names
DHE4_HUMAN; GDH 2; GDH; GDH2; GLUD2; GLUDP1; Glutamate dehydrogenase 2; Glutamate dehydrogenase 2 mitochondrial; Glutamate dehydrogenase pseudogene 1; mitochondrial
Species
Homo sapiens (Human)
Expression Region
54-558
Target Protein Sequence
SELVADR EDDPNFFKMV EGFFDRGASI VEDKLVKDLR TQESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTENELEK ITRRFTMELA KKGFIGPGVD VPAPDMNTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFR DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKPYEGS ILEVDCDILI PAATEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NILVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLLSV QESLERKFGK HGGTIPIVPT AEFQDSISGA SEKDIVHSAL AYTMERSARQ IMHTAMKYNL GLDLRTAAYV NAIEKVFKVY SEAGVTFT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
Gene References into Functions
  1. hGDH2 evolution bestowed large human neurons with enhanced glutamate metabolizing capacity, thus strengthening cortical excitatory transmission. PMID: 26399640
  2. This study demonstrated that hGDH2 expression increases capacity for uptake and oxidative metabolism of glutamate, particularly during increased workload and hypoglycemia. PMID: 28032919
  3. This proliferation requires glutamate dehydrogenase 2, which synthesizes glutamate from ammonia and alpha-ketoglutarate and is expressed in MCF7 and T47D cells. Our findings provide insight into how cancer cells survive under glutamine deprivation conditions and thus contribute to elucidating the mechanisms of tumor growth. PMID: 29146184
  4. The expression of GLUD2 was identified in the cellular and subcellular compartments of numerous tissues. PMID: 27422263
  5. Notably, the introduction of GLUD2 did not affect glutamate levels in mice, consistent with observations in the primates. Instead, the metabolic effects of GLUD2 center on the tricarboxylic acid cycle, suggesting that GLUD2 affects carbon flux during early brain development, possibly supporting lipid biosynthesis. PMID: 27118840
  6. IDH1(R132H) exhibits a growth-inhibitory effect that is abrogated in the presence of glutamate dehydrogenase 2 (GLUD2), a hominoid-specific enzyme purportedly optimized to facilitate glutamate turnover in human forebrain. PMID: 25225364
  7. hGDH2 can operate efficiently in the relatively acidic environment that prevails in astrocytes following glutamate uptake [review] PMID: 24352816
  8. [review] Whereas GDH2 in most mammals is encoded by a single functional GLUD1 gene expressed widely, humans have acquired through retroposition an X-linked GLUD2 gene that encodes a highly homologous isoenzyme GDH2 expressed in testis and brain. PMID: 21420458
  9. GLUD2 glutamate dehydrogenase is expressed in neural and testicular supporting cells PMID: 20194501
  10. A gain-of-function rare polymorphism in hGDH2 hastens the onset of Parkinson's disease in hemizygous subjects. PMID: 19826450
  11. amino acid changes, acting in concert with Arg443Ser and Gly456Ala, ought to be responsible the unique properties of the brain-specific human isoenzyme PMID: 17924438
  12. Mitochondrial targeting specificity of GLUD2 is due to an amino acid substitution in the mitochondrial targeting sequence soon after the duplication event in the hominoid ancestor approximately 18-25 million years ago. PMID: 18688271
  13. study of molecular mechanisms regulating hGDH2 function by creating & analyzing mutants with single amino acid substitutions in the regulatory domain (antenna, pivot helix) of the protein PMID: 19393024
  14. GDH2 localizes mainly to mitochondria and to a lesser extent to the endoplasmic reticulum of cells PMID: 19428807

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Subcellular Location
Mitochondrion matrix.
Protein Families
Glu/Leu/Phe/Val dehydrogenases family
Tissue Specificity
Expressed in retina, testis and, at a lower level, brain.
Database Links

HGNC: 4336

OMIM: 300144

KEGG: hsa:2747

STRING: 9606.ENSP00000327589

UniGene: Hs.368538

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