Recombinant Human HLA class II histocompatibility antigen, DO alpha chain (HLA-DOA), partial

Code CSB-YP356784HU
MSDS
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Source Yeast
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Code CSB-EP356784HU
MSDS
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Source E.coli
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Code CSB-EP356784HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP356784HU
MSDS
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Source Baculovirus
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Code CSB-MP356784HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HLA-DOA
Uniprot No.
Alternative Names
HLA-DOA; HLA-DNA; HLA-DZAHLA class II histocompatibility antigen; DO alpha chain; MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.
Gene References into Functions
  1. Contribution of a Non-classical HLA Gene, HLA-DOA, to the Risk of Rheumatoid Arthritis PMID: 27486778
  2. significant association was found between white wine liking and rs9276975:C>T polymorphism in the HLA-DOA gene. PMID: 25758996
  3. TAP2, HLA-DOA, HLA-DOB, and tapasin loci are novel candidate regions for susceptibility to HCV infection and viral clearance in the Chinese population PMID: 25874709
  4. HLA-DO regulates surface presentation of human leukocyte antigen class II-restricted antigens on B cell malignancies. PMID: 24530695
  5. HLA-DOA, HLA-DRA, and HLA-DQA1 associated with spontaneous autoimmunity PMID: 24384427
  6. results show that HLA-DO inhibits HLA-DM function by acting as a substrate mimic, and the findings also limit the possible functional roles for HLA-DO in antigen presentation PMID: 23222639
  7. analysis of the HLA-DO/HLA-DM complex by FRET and mutagenesis PMID: 22733780
  8. HLA-DOA mutations were analyzed for association with autoimmune diseases and B-cell leukemias. PMID: 12439622
  9. In transgenic mice expressing human HLA-DOA & HLA-DOB from a DC-specific promoter, as DO increased, A(b)-CLIP increased & class II-peptides decreased. DO impaired H-2M function, causing Ag-specific downmodulation of class II Ag processing/presentation. PMID: 15265882
  10. These results show that transduced DO modulates antigen presentation in human mature MoDC, evoking the possible use of this chaperone for immunotherapy. PMID: 15817706
  11. four novel HLA-DOA alleles, DOA*010106, DOA*0102, DOA*0103, and DOA*0104N, were identified by sequence-based typing PMID: 16101837
  12. minor allele of the SNP rs9296068 is significantly associated with liver transplantation rejection and with enhanced B-lymphocyte participation in rejection, likely because of a dysfunctional HLA-DOA gene product. PMID: 18639552
  13. HLA-DOA polymorphisms are associated with Diabetes Mellitus, Type 1. PMID: 19458622

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Subcellular Location
Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER.
Protein Families
MHC class II family
Database Links

HGNC: 4936

OMIM: 142930

KEGG: hsa:3111

STRING: 9606.ENSP00000229829

UniGene: Hs.631991

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