Recombinant Human Histone H1t (HIST1H1T)

Code CSB-YP010383HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP010383HU
MSDS
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Source E.coli
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Code CSB-EP010383HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010383HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP010383HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HIST1H1T
Uniprot No.
Alternative Names
H1 histone family; member T (testis specific); H1FT ; H1T ; H1T_HUMAN; Hist1h1t; Histone cluster 1; H1t ; Histone H1t; Testicular H1 histone
Species
Homo sapiens (Human)
Expression Region
2-207
Target Protein Sequence
SETVPAASA SAGVAAMEKL PTKKRGRKPA GLISASRKVP NLSVSKLITE ALSVSQERVG MSLVALKKAL AAAGYDVEKN NSRIKLSLKS LVNKGILVQT RGTGASGSFK LSKKVIPKST RSKAKKSVSA KTKKLVLSRD SKSPKTAKTN KRAKKPRATT PKTVRSGRKA KGAKGKQQQK SPVKARASKS KLTQHHEVNV RKATSKK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination. Histones H1 act as linkers that bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration (Probable).
Gene References into Functions
  1. These results suggested that the testis-specific linker histone, H1T, possesses a specific function to produce the chromatin architecture required for proper chromosome regulation, such as homologous recombination. PMID: 26757249
Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H1/H5 family
Tissue Specificity
Testis-specific.
Database Links

HGNC: 4720

OMIM: 142712

KEGG: hsa:3010

STRING: 9606.ENSP00000341214

UniGene: Hs.533293

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