Recombinant Human Integrin beta-4 (ITGB4),Yeast, partial,Yeast

Code CSB-YP011887HU
MSDS
Size
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
CD 104; CD104; CD104 antigen ; gp150; Integrin beta 4 subunit; Integrin beta-4; ITB4_HUMAN; ITG B4; ITGB 4; Itgb4
Species
Homo sapiens (Human)
Source
Yeast
Protein Length
Extracellular domain
Tag Info
N-terminal His-tagged
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.
Gene References into Functions
  1. High ITGB4 expression is associated with drug resistance in colorectal cancer. PMID: 30106452
  2. ITGB4 plays a tumorigenic and pro-metastatic role mediated by Slug PMID: 28084395
  3. correlation analysis indicated that the expression of ARRDC3 was negatively correlated with ITGbeta4 in clinical prostate cancer (PCa) tissues and cell lines. Our data revealed that ARRDC3 can serve as a tumor suppressor to inhibit PCa progression and an independent marker to predict the risk of biochemical recurrence and metastasis after radical resection of PCa. PMID: 28782483
  4. ITGB4(+) cancer stem cell (CSC)-enriched mesenchymal cells reside in an intermediate epithelial/mesenchymal phenotypic state. PMID: 28270621
  5. These findings suggest that the integrin beta4-FAK/Src signaling axis may play a crucial role in clonorchiasis-associated cholangiocarcinoma metastasis during tumor progression. PMID: 28286026
  6. MUC5AC interacts with integrin beta4 that mediates phosphorylation of FAK at Y397 leading to lung cancer cell migration. PMID: 26751774
  7. a significant reduction in the protein distribution of collagen IV (P<0.0001), collagen VII (P<0.001), collagen XVII (P<0.01), integrin beta4 (P<0.001) and laminin-332 (P<0.0001) in intrinsically aged skin. PMID: 27013376
  8. For the in vivo experiment, miR-182-5p overexpression also promoted the growth and progression of prostate cancer tumors. In this regard, we suggest that miR-182-5p may be a key androgen receptor-regulated factor that contributes to the development and metastasis of Chinese prostate cancers and may be a potential target for the early diagnosis and therapeutic studies of prostate cancer. PMID: 27109471
  9. data indicate that the beta4 integrin/FAK complex and subsequent FAK activation are essential regulators during the tumorigenicity of colon cancer PMID: 27178753
  10. This work investigates alpha6beta4 integrin genetic variations (A380T and R1281W) and breast cancer risk in an Argentinian population. No subjects carrying the R1281W mutation in the ITGB4 gene were found and this absence could reflect its high deleterious impact on proteins, so it would be eliminated from the population by natural selection. PMID: 27763564
  11. Herein, we present a female newborn with lethal Junctional epidermolysis bullosa with pyloric atresia caused by a novel beta4 integrin mutation. PMID: 27186702
  12. analysis of deletions in ITGB4 gene causing epidermolysis bullosa with pyloric atresia [case series] PMID: 26739954
  13. ITGB4 is overexpressed in hepatocellular carcinoma tissues and promotes metastases of HCC by conferring anchorage independence through EGFR-dependent FAK-AKT activation. PMID: 26996299
  14. High vimentin and low beta4 integrin protein levels are associated with poor survival in oral squamous carcinoma patients. PMID: 26646105
  15. Endothelial cell overexpression of mutant ITGB4 with specific tyrosines mutated to phenylalanine (Y1440, Y1526 Y1640, or Y1422) resulted in significantly attenuated CS-induced cytokine expression. PMID: 26572585
  16. This study describes a pro-metastatic EGFR/Src-dependent beta4 integrin/FAK complex that is involved in breast cancer malignancy and is a novel therapeutic target for triple-negative breast cancer. PMID: 26549523
  17. These data suggest that the p53(R248) mutant endows ovarian cancer cells with increased adhesiveness and that integrin beta4 and Akt signalling are associated with the mutation-enhanced ovarian cancer-mesothelial cell adhesion. PMID: 26223322
  18. Changes in beta-integrins gene expression in human prostate cancer cells following ITGB4 knockdown. PMID: 26395630
  19. PKD2 and RSK1 regulate integrin beta4 phosphorylation at threonine 1736 to stabilize keratinocyte cell adhesion and its hemidesmosomes. PMID: 26580203
  20. Data indicate that IGF binding protein-3 (IGFBP-3) reduced transcription of a variety of integrins, especially integrin beta4. PMID: 25945837
  21. Analysis showed that ITGB4 and VCL were upregulated in exosomes derived from taxane-resistant prostate cancer cells suggesting them as useful markers for progression of prostate cancer associated with taxane-resistance. PMID: 25997717
  22. Case Report: pyloric atresia-junctional epidermolysis bullosa syndrome with splicing abnormality of ITGB4 due to nucleotide substitutions far from splice site. PMID: 25728941
  23. ITGB4 stimulation leads to recruitment of 12-LOX from the cytosol to the membrane. PMID: 26037302
  24. The third and fourth fibronectin type III domains (FnIII-3,4) emerge as a structural and functional unit within the integrin beta 4. PMID: 25849406
  25. find that both Syk and LMP2A have an effect on ITGbeta4 cell surface expression PMID: 25531330
  26. Case Report: ITGB4 germline mutations in junctional epidermolysis bullosa without pylori atresia but profound genito-urinary involvement. PMID: 24807042
  27. Mutations in the 5' flanking region result in reduced integrin beta4 expression, which is related to increased risk of asthma. PMID: 24740264
  28. By a loss-of-function assay, we elucidate a novel feed-forward signaling pathway, integrin beta4 --> PI3K --> Akt --> FAK, by which TR3 mediates HUVEC migration. Furthermore, TR3/Nur77 regulates the expression of integrin beta4 by targeting its promoter activity. PMID: 25326539
  29. Data indicate that knockdown of integrin beta4 (ITGB4) reduced the migratory and invasive ability of pancreatic ductal adenocarcinoma cells. PMID: 25599535
  30. miR-21 is a key player in oncogenic EMT, its overexpression is controlled by the cooperation of genetic and epigenetic alterations, and its levels, along with ITGbeta4 and PDCD4 expression, could be exploited as a prognostic tool for CRC metastasis. PMID: 24149370
  31. underexpressed in circulating (4D culture) cells relative to 2D culture cells PMID: 25234746
  32. the diminishment of crosstalk between phosphorylated FAK and ITGB4 due to the down-regulation of Gli family transcription factors might play a pivotal role for inhibiting ovarian cancer progression PMID: 24533083
  33. Data indicate that sphingosine 1-phosphate (S1P) and hepatocyte growth factor (HGF) induced transloaction of integrin beta4, S1P receptors S1PR2 and S1PR3 to endothelial cell membrane caveolin-enriched microdomains (CEMs). PMID: 24851274
  34. Both annexin A7 and integrin beta4 were essential for small molecule, 6-amino-2, 3-dihydro-3-hydroxymethyl-1, 4-benzoxazine-induced autophagy. PMID: 24007983
  35. The expression of MPS-1 and ITGB4 is associated with poor outcomes in gastric cancer patients. PMID: 23803695
  36. beta4 and beta1 integrin protein and mRNA expression is elevated in PKP2-silenced keratinocytes. PMID: 23884246
  37. beta4 integrin rs8669 genotyping may help to identify a subgroup of HER-3-negative, K-RAS wild-type colorectal cancer patients who are more likely to benefit from anti-EGFR treatment. PMID: 23617461
  38. study reports two new non-Herlitz junctional epidermolysis bullosa cases carrying two novel ITGB4 mutations - c.3040C>T in exon 26 and c.4975G>T in exon 37 PMID: 23013259
  39. Post-translational regulation of beta4 by PTHrP (Parathyroid hormone-related protein) is mediated via attenuation of its proteolytic cleavage by caspases. PMID: 23499737
  40. Mechanistic studies indicated that mutant ITGB4 fails to promote transactivation of ErbB2 and c-Met in prostate tumor progenitor cells and cancer cell lines. PMID: 23348745
  41. S1PR1 and ITGB4 transactivation are rate-limiting events in the transduction of HGF signals via a dynamic c-Met complex resulting in enhanced EC barrier integrity PMID: 23212923
  42. Most actinic cheilitis cases showed reduced expression of integrin beta4 and superficially invasive squamous cell carcinoma lacked intergrin beta4 in the invasive front. PMID: 22917688
  43. invasive breast cancer cells confer an anoikis-resistant phenotype on myofibroblasts during tissue remodeling by inducing laminin-332 upregulation and integrin beta4 neoexpression PMID: 22673183
  44. MAPK activation is increased when [Ca(2+)](i) is increased, suggesting that calcineurin activates an additional mechanism that counteracts MAPK-induced beta4 phosphorylation. PMID: 22865863
  45. Silencing of ITGB4 in airway epithelial cells resulted in impaired antigen presentation processes and suppressed T cell proliferation. PMID: 22545078
  46. Mutational analysis disclosed compound heterozygosity for two novel nonsense mutations in the ITGB4 gene: c.600dupC/p.F201fsX14 and c.2533C>T/p.Q845X. PMID: 21969027
  47. the expression of integrin beta(4) is upregulated to sensitize the cells to low concentrations of netrin-4 for maintaining cell proliferation. PMID: 22496621
  48. beta4 can regulate SPARC expression and that SPARC is an effector of beta4-mediated invasion. PMID: 22308039
  49. AexU effector protein of Aeromonas veronii is a novel type three secretion system effector which specifically inactivates Rac1 to disrupt actin cytoskeleton and has an alternative cytotoxic pathway through beta4-integrin mediation. PMID: 21963679
  50. beta4 serine phosphorylation may have important role during squamous cell carcinoma invasion by destabilizing hemidesmosomes and facilitating migration. PMID: 21769085

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Involvement in disease
Epidermolysis bullosa letalis, with pyloric atresia (EB-PA); Generalized atrophic benign epidermolysis bullosa (GABEB)
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note=Colocalizes with DST at the leading edge of migrating keratinocytes.
Protein Families
Integrin beta chain family
Tissue Specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Database Links

HGNC: 6158

OMIM: 147557

KEGG: hsa:3691

STRING: 9606.ENSP00000200181

UniGene: Hs.632226

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