Recombinant Human Interleukin-4(IL4)

Code CSB-YP011659HU
Size US$2010
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names IL4
Uniprot No. P05112
Research Area Immunology
Alternative Names B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; B-cell stimulatory factor 1; BCGF 1; BCGF1; Binetrakin; BSF-1; BSF1 ; IGG1 induction factor; IL 4; IL-4; IL4; IL4_HUMAN; Il4e12; Interleukin 4; Interleukin 4 variant 2; Interleukin 4; isoform 1 ; Interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra
Species Homo sapiens (Human)
Source Yeast
Expression Region 25-153aa
Target Protein Sequence HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 17.0kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Participates in at least several B-cell activation processes as well as of other cell types
Gene References into Functions
  1. IL-33 has a role in the pathogenesis of autoimmune hepatitis (AIH) and affects the expression of IL-4, IL-17A, and of hypergammaglobulinemia PMID: 30034292
  2. Interleukin-4 induces a CD44high /CD49bhigh PC3 subpopulation with tumor-initiating characteristics. PMID: 29236307
  3. Allelic polymorphism C590T of the gene IL-4 is the most possible genetic marker of high predisposition to development of recurrent episodes of acute obstructive bronchitis in children. PMID: 30480406
  4. certain single nucleotide polymorphisms of IL4 gene could predispose individuals to recurrent aphthous stomatitis PMID: 29985726
  5. Germline variants in IL4 gene are associated with prostate cancer. PMID: 29298992
  6. The role of IL-4 in psoriasis. PMID: 28064550
  7. By establishing that IL-4 is posttranslationally regulated by TRX-promoted reduction of a disulfide bond, our findings highlight a novel regulatory mechanism of the type 2 immune response that is specific to IL-4 over IL-13. PMID: 30104382
  8. IL-4 polymorphisms might be associated with Kawasaki disease in an Iranian population. PMID: 28036156
  9. association between root resorption and IL4 gene polymorphisms was observed. PMID: 28617966
  10. these results demonstrated that variable number tandem repeat polymorphism in the IL-4 gene is associated with diabetic peripheral neuropathy in type 2 diabetes patients with coexisting cardiovascular disease PMID: 29182400
  11. serum antibodies against HP-NAP represent a state of risk, which is further exacerbated in IL-4 -590 T carriers. PMID: 27677314
  12. Polymorphisms at IL10 (-1082 G>A), IL4 (-589 C>T), CTLA4 (+49A>G), and DAO (+8956 C>G) genes were studied in 55 cases. PMID: 28750137
  13. Defective sirtuin-1 was found to increase IL-4 expression through acetylation of GATA-3 in patients with severe asthma compared with healthy controls. PMID: 26627546
  14. The results confirmed that the IL-4-590C/T polymorphism is correlated with the onset of RA and that carrying the T-allele can significantly increase the risk of rheumatoid arthritis in the Chinese Han population. PMID: 28975976
  15. the IL-4-590 C>T polymorphism does not influence the development of head and neck cancer. PMID: 29185028
  16. the present study suggests that IL-4 polymorphisms might play a role in susceptibility to inflammatory bowel disease and its major subtypes in the Iranian population PMID: 28872970
  17. IL-4R plays an important role in regulating hepatocellular carcinoma (HCC)cell survival and metastasis, and regulates the activity of the JAK1/STAT6 and JNK/ERK1/2 signaling pathways. We therefore suggest that IL-4/IL-4R may be a new therapeutic target for HCC PMID: 28665449
  18. IL-4 rs2227288 and IL-10 rs1800872 may contribute to an increased risk for virus-induced encephalitis. Through use of direct sequencing, we showed that genotypes of IL-4 rs2227288 and IL-10 rs1800872 may have particular host susceptibility to virus-induced encephalitis. IL-4 rs2227283 and IL-10 rs1800871 have no correlation in with risk of virus-induced encephalitis (both P>0.05) PMID: 28935853
  19. IL-4 and IL-8 genetic polymorphisms determine susceptibility to chronic Aggregatibacter actinomycetemcomitans periodontitis. PMID: 28859277
  20. The data indicate that USP4 interacts with and deubiquitinates IRF4, and also stabilizes IRF4 protein and promotes IRF4 function to facilitate IL-4 expression in Th2 cells, which may be related to the pathological process of rheumatic heart disease. PMID: 28791349
  21. Results show that higher levels of black carbon (BC) were associated with lower methylation of IL4 promoter CpG-48 5 days later and increased FeNO. The magnitude of association between BC exposure and demethylation of IL4 CpG-48 measured 5 days later appeared to be greater among seroatopic children, especially those sensitized to cockroach allergens. PMID: 28588744
  22. Association between the TT-genotype of IL-4 rs2070874 polymorphism and a severe phenotype of viral-induced wheeze further underlines the role IL-4 plays in the inflammation pathway leading to viral respiratory infections. PMID: 28950434
  23. the effects of IL4 gene polymorphisms on cancer risk may vary by cancer type and by ethnicity. PMID: 28656227
  24. these results showed that allergy responses further accelerated the IL-4-induced inhibition of tumor development through the activation of STAT6 pathways. PMID: 28587956
  25. we identified a subgroup of CVID patients with defective IL-4 signaling in T cells, with severe clinical features of inflammation and autoimmunity. PMID: 28476239
  26. The difference between allelic and genotypic frequencies of interleukin-4 (-590C/T) between patients and controls was not significant (p = 0.46). PMID: 29372577
  27. Data suggest that miRNA-340/429, which targeted IL-4, might be a potential approach for cancer treatment. PMID: 27895317
  28. this paper shows that expression of non-secreted IL-4 is associated with histone deacetylase inhibitor-induced cell death, histone acetylation and c-Jun regulation in gamma/delta T-cells PMID: 27556516
  29. Human CCL1 gene is selectively targeted by AhR in M(IL-4) macrophage. IL-4-induced epigenetic modification potentiates AhR-mediated CCL1 expression. PMID: 27888289
  30. Liver IL-4 mRNA is downregulated in patients with pancreatic cancer and cachexia. PMID: 27897439
  31. Low IL4 expression is associated with melanoma. PMID: 26993600
  32. In this review, we discuss the molecular mechanisms driving IL-4 production in Th2 and Tfh cells. PMID: 27072069
  33. In this review, we discuss the structural details of IL-4 and IL-4Ralpha subunit and the structural similarities between IL-4 and IL-13. We also describe detailed chemistry of type-I and type-II receptor complexes and their signaling pathways. Furthermore, we elaborate the strength of type-II hetero dimer signals in response to IL-4 and IL-13. PMID: 27165851
  34. IL-4 and IL-17 modulate the functional activity of phagocytes in the maternal blood, cord blood, and colostrum of diabetic mother. PMID: 29135055
  35. There was no significant difference in serum level of IL-4 between children with MPP (Mycoplasma pneumoniae pneumonia)and those with non-MPP. Among children with MPP, we found similar level of IL-4 regardless of the personal and family history of allergy and asthma or the presence of wheezing. PMID: 28057814
  36. The functional promoter polymorphisms IL4-590C/T and IL6-174G/C, which affect the IL-4 and IL-6 levels in north Indian subjects, were associated with kidney dysfunction and CKD PMID: 27996163
  37. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. PMID: 29262348
  38. CGRP and IL-4 positively regulated APN/CD13 expression and activity in psoriatic fibroblasts. PMID: 28387421
  39. Results from these two large randomized aerobic exercise intervention trials suggest that aerobic exercise does not alter IL-10 or IL-4 in a manner consistent with chronic disease and cancer prevention. PMID: 27485297
  40. IL-4 signaling up-regulates the IL-25 axis in human monocytic cells, and IL-25 may provide autocrine signals in monocytes and macrophages to sustain IL-17Rb expression and predispose to alternative activation. PMID: 28421819
  41. IL4 VNTR B2 allele was only significantly associated with overall adiposity status before adjusting for ethnicity. PMID: 28293435
  42. Our results showed the role of IL4 in promoting breast cancer aggressiveness PMID: 28400477
  43. These data identified the IL-4/CXCL12 loop as a previously unrecognized pathway involved in lymphoid stroma polarization and as a potential therapeutic target in Follicular lymphoma patients. PMID: 28202459
  44. IL-4 genetic variations associated with susceptibility to or protection against chronic periodontitis are directly associated with influencing the response of immune cells to periodontopathogens PMID: 28114408
  45. in HIV/AIDS patients under antiretroviral therapy, IL-4 and IL-10 levels were significantly lower in lipodystrophy vs. non-lipodystrophy PMID: 28189545
  46. autologous CD4(+) T cells that are exposed to EVs from CD40/IL-4-stimulated CLL cells exhibit enhanced migration, immunological synapse signaling, and interactions with tumor cells. PMID: 27118451
  47. IL-4 substantially restores CD79b protein expression, sIgM expression, and BCR signaling. PMID: 27226435
  48. Tandem repeat polymorphisms of IL4 is associated with the severity of chronic periodontitis. PMID: 28053321
  49. Keratinocyte gene expression is critically shaped by IL-4, altering cell fate decisions, which are likely important for the clinical manifestations and pathology of allergic skin disease PMID: 27554818
  50. Inhibition of protein kinase C zeta expression in prostate cancer cells promoted chemotaxis of peripheral macrophages and acquisition of M2 phenotypic features. These results were further supported by the finding that silencing of endogenous protein kinase C zeta promoted the expression of prostate cancer cell-derived interleukin-4 and interleukin-10 PMID: 28631559
  51. Studies in colonic T84 cell monolayers revealed that barrier disruption by the colitis-associated Th2-type cytokines, IL-4 and IL-13, down-regulates matriptase as well as prostasin through phosphorylation of the transcriptional regulator STAT6 PMID: 28490634
  52. IL-4 induces demethylation of specific CpG sites within the pendrin promoter. These epigenetic alterations are cell type specific, and may in part dictate pendrin mRNA transcription PMID: 28365704
  53. results suggest that certain polymorphisms of IL4 and IL4R may affect susceptibility to gastrointestinal cancer [meta-analysis] PMID: 28142034
  54. the IL-4 -590C/T polymorphism may contribute to atopic dermatitis susceptibility in the overall population and children, especially for Asian children [meta-analysis] PMID: 28169853
  55. this study shows that that IL-12 and IL-4 govern group 2 innate lymphoid cells functional identity and that their imbalance results in the perpetuation of type 1 or type 2 inflammation PMID: 27111145
  56. these data highlight two novel regulatory proteins that could be therapeutically manipulated to limit IL-4-induced IRS-2 signaling and polarization of M2 macrophages in allergic inflammation. PMID: 27742835
  57. TGFbeta1 may be key in controlling the glycosylation of IgA1, in part via the downregulation of C1GalT1 and Cosmc. TGF-beta1 mimics the effect of IL-4 on the glycosylation of IgA1 by downregulating C1GalT1 and Cosmc. PMID: 28035353
  58. The IL-4 -33 CT genotype is negatively associated with the occurrence of aggressive periodontitis. PMID: 28392616
  59. findings provide novel insight into how dysregulated expression of SOCS increases IL-4 responses in allergic monocytes, and this may represent a new therapeutic avenue for managing allergic disease. PMID: 27507812
  60. We observed that the TT genotype, compared to the CC genotype, of IL-4 C-590T harbored a lower risk of pre-eclampsia. The CT+TT genotype, compared to the CC genotype, harbored a lower risk of pre-eclampsia in the dominant model. In the recessive model, the TT genotype harbored a lower risk of pre-eclampsia. No significant correlation was observed between the IL-4 C+33T and G-1098T polymorphism. PMID: 28387873
  61. Results found IL4 -33 T/T genotype to be associated with increased incidence of acute kidney injury after liver transplantation. PMID: 27003899
  62. IL-4 signaling plays a crucial role in the regulatory functions of SOCS1 in apoptosis and inflammation in human osteoarthritic chondrocytes. These findings provide a potential therapeutic target for the clinical treatment of osteoarthritis (OA) PMID: 28373981
  63. Increasing risk of asthma was associated with C allele and the CC genotype in IL-4 590. PMID: 28386156
  64. These data suggest that the expression of IL-4 is inversely proportional to hepatitis C virus load of viremia, and this connection is due to the high level of mutant genotype IL-4-589/TT in infected patients located in gene promoter and inhibits gene expression. PMID: 28368861
  65. Study indicates that endogenously expressed IL-4 and IL-4Ralpha contribute to the malignant phenotype of pancreatic cancer cells by activating diverse pro-oncogenic signaling pathways. PMID: 28350325
  66. The role of Th2 cytokines (IL-4, IL-13) and STAT6 in Th1/Th2 imbalance. PMID: 26781462
  67. We investigated the association between the interleukin 4 gene -590C;T polymorphism and forced expiratory volume. IL4 -590C;T polymorphism is related to bronchial asthma in Uighur children. Furthermore, this genetic variant can result in raised IgE levels and decreased FEV1 values, suggesting that both factors are associated with bronchial asthma in Uighur children. PMID: 27525870
  68. in homozygous female carriers of high expression alleles capital TE, Cyrillic (IL-4 C-589T) and G (IL-10 G-1082A), neuroticism scores were higher (p = 0.045 and p = 0.08, respectively). In turn, extraversion scores were significantly higher in both male and female carriers of heterozygous variants CT and GA (p = 0.01). PMID: 28064311
  69. The IL4_VNTR P1 allele, P2P2 genotypes, and IL4-VNTR_IL6-174CG P2P1-GG genotypes are common in southern Turkey, and carriers of these polymorphisms are susceptible to brucellosis. PMID: 27169944
  70. this study shows that IL-4 gene polymorphism is associated with psoriasis phenotypes in India PMID: 28027921
  71. NOS3 27-bp and IL4 70-bp VNTR Polymorphisms Do Not Contribute to the Risk of Sickle Cell Crisis PMID: 27400089
  72. Suggest that IL-4 gene polymorphisms may play a role in the response to the HBV vaccine in Korean infants. PMID: 28051794
  73. IL-4 polymorphisms associated with diminished serum IL-4 levels may be partially responsible for ankylosing spondylitis development in the Chinese population. PMID: 27813599
  74. A case-control study was performed to evaluate the relationship between asthma risk and the IL-4 rs2243250 (589 C/T) and rs2070874 (107 T/C), and IL-4 receptor (IL-4R) rs1801275 (576 Q/R) polymorphisms in 317 childhood asthma patients and 351 healthy children as controls. PMID: 27819719
  75. These findings suggest that enhanced miR-342-3p expression is a component of a negative feedback loop controlling excessive macrophage proliferation during Th2-type inflammatory responses. Moreover, study indicates that miR-342-3p likely plays a pro-apoptotic role in such cells, thereby providing a negative feedback arm to IL-4-dependent macrophage proliferation. PMID: 27245778
  76. No significant association between IL4 rs2070874 or IL13 rs1800925 and chronic periodontitis (CP) was found, while the frequencies of IL4 rs2243248 and two haplotypes C-G-T and C-T-T showed significant differences between 440 moderate or severe CP patients and 324 healthy controls. PMID: 27195298
  77. IL4R gene may affect the survival of glioma patients. PMID: 27495027
  78. Polymorphisms in promoter region of IL-4 but not IL-4RA genes confer susceptibility to juvenile idiopathic arthritis and may predispose individuals to adaptive immune responses. PMID: 26951255
  79. IL-4 alters human skin mast cell (MCs) after long-term exposure mimicking chronic disorders by strengthening MC numbers and intensifying processes associated with allergic inflammation. PMID: 27650274
  80. this study shows that IL-4 gene polymorphisms may be associated with Graves disease and Hashimoto's thyroiditis susceptibility PMID: 27100882
  81. IL-4 (intron 3) and IL-10 (-1082) gene polymorphisms are not significantly associated with susceptibility to rheumatic heart disease, but they might play a role in the pathogenesis of patients with mitral valve disease. PMID: 26506984
  82. the IL-4 gene polymorphism (C-590T) had no relationship with susceptibility to the Atopic Dermatitis among Saudi children PMID: 27018548
  83. plasma IL-4 and IL-6 were positively correlated with the radiographic severity of knee osteoarthritis. PMID: 27122451
  84. Identify the IL4 C allele and CC genotype as susceptibility genes in Iranian children with autoimmune hepatitis. PMID: 26735262
  85. Significant association was found of the IL-4 gene single nucleotide polymorphism in children Iranian population. PMID: 26228820
  86. Genotyping of IL4 T-33C in recipients might be useful to stratify the liver transplant recipients according to their risk of acute cellular rejection PMID: 26212175
  87. The aim of this study was to evaluate the impact of the polymorphisms within interleukin 4 (rs2243250, rs2227284), interleukin 4 receptor alpha chain (rs1805010, rs1805011) and interleukin 13 (rs20541) genes on the incidence of allergic phenotype in Polish pediatric population. PMID: 26426602
  88. IL-4 -590 and IL-4R Q551R gene polymorphisms are not associated with the susceptibility and the clinical pattern of alopecia areata in Saudi patients. PMID: 25853541
  89. One IL-4R gene polymorphism (rs1801275) was associated with acute rejection. In addition, a separate IL-4R SNP (rs2107356) was statistically associated with graft dysfunction after kidney transplantation. PMID: 27234743
  90. This meta-analysis indicates that a polymorphism of IL4 rs2243250 (C/T) reduces the risk of multiple sclerosis in Caucasian populations. PMID: 27000232
  91. findings demonstrate a negative regulatory role of IL-4 in gammadelta T cell-mediated anti-tumor immunity. PMID: 25942601
  92. Our results suggest that IL13 rs20541, IL4 rs2243250, ADRB2 rs1042713, and FCER1B rs569108, four SNPs with significant sole effect on asthma, interact to confer a higher risk for the disease in Chinese Han children. PMID: 26613553
  93. Result found the 590T and 589T alleles of IL-4 gene may be associated with high levels of serum IL-4, which may increase the risk of atopic dermatitis in children. PMID: 27212784
  94. Interleukin-4 used with GM-CSF and TNF-alpha during the maturation of DCs in vitro impaired dendritic cells (DC) functions. PMID: 27080531
  95. In Chinese Han children, the IL-4 -590C/T and -33 C/T polymorphisms were associated with the susceptibility to Respiratory syncytial virus (RSV) infection and the severity of the infection. PMID: 26289664
  96. investigated five SNPs (C-589T and C-33T of IL4, G+2044A of IL13, A+1902G of IL4Ralpha, and A+896G of TLR4) in patients with adult onset asthma to evaluate their role in manifestation and severity of asthma PMID: 26831416
  97. IL4 expression in follicular fluid and follicular fluid lymphocytes of PCOS patients is not significantly different from controls. PMID: 26751822
  98. A constitutively activated form of STAT6 is able to bypass IL-4 upstream signalling and instruct dendritic-cell-specific functional DNA methylation changes PMID: 26758199
  99. interleukin-4 regulates hematopoietic lineage choice by activating the JAK3-STAT6 pathway, which causes dendritic-cell-specific DNA demethylation PMID: 26829670
  100. The C allele of the +33 (C/T) polymorphism in the IL4 gene was associated with susceptibility to dental implant loss in Brazilians in the studied population. PMID: 25343318

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Involvement in disease Ischemic stroke (ISCHSTR)
Subcellular Location Secreted
Protein Families IL-4/IL-13 family
Database Links

HGNC: 6014

OMIM: 147780

KEGG: hsa:3565

STRING: 9606.ENSP00000231449

UniGene: Hs.73917

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