Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ILF2

Uniprot No.

Q12905

Research Area

Transcription

Alternative Names

HGNC:6037; ILF 2; ilf2; ILF2_HUMAN; Interleukin enhancer binding factor 2; Interleukin enhancer binding factor 2; 45kDa; Interleukin enhancer-binding factor 2; MGC8391; NF 45; NF45; Nuclear factor of activated T cells 45 kDa; Nuclear factor of activated T-cells 45 kDa; PRO3063

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-390aa

Target Protein Sequence

MRGDRGRGRGGRFGSRGGPGGGFRPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEENTEEPPQGEEEESMETQE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

70.1kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human ILF2 protein synthesis in e.coli cells necessitates the incorporation of a DNA fragment encoding the Human ILF2 protein (1-390aa) into a plasmid vector, followed by the transformation of this vector into e.coli cells. After screening for positive cells, they are cultured and induced to express the ILF2 protein. The protein carries a N-terminal GST tag. Cell lysis is performed to gather the recombinant Human ILF2 protein, which undergoes affinity purification and is then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human ILF2 protein reaches up to 90%.

Target Background

Function

Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus.

Gene References into Functions

this study identifies a new role of ILF2 in the regulation of the NLRP3 inflammasome. PMID: 29655789
High expression of ILF2 was tightly correlated with lymph node metastasis, pathological stage and poor clinical prognosis in gastric cancer. PMID: 28831206
ILF2 interacts with RNA-binding proteins involved in the DNA damage response. PMID: 28669490
The data suggest that features of both the N- and C-termini of NF90 participate in the heterodimerization with NF45 and that the formation of NF90-NF45 changes the conformation of NF90's RNA-binding motifs to a status in which the co-operative interplay of the RNA-binding motifs is optimal. PMID: 28062840
NF90-NF45 stimulates an elevation of EGF receptor levels via the suppression of miR-7 biogenesis, resulting in the promotion of cell proliferation in Hepatocellular Carcinoma. PMID: 27519414
The results revealed that ILF2 is regulated by miR-7 and suggest that downregulation of miR-7 may be an important factor for the ILF2 overexpression in pancreatic carcinoma. PMID: 28259961
Deletion of the RNA binding domains of NF45 and NF90 diminished the enhancement of HIV infection and gene expression. PMID: 26891316
NF45 overexpression is associated with poor prognosis and enhanced cell proliferation of pancreatic ductal adenocarcinoma. PMID: 26276310
Upregulated expression of ILF2 in non-small cell lung cancer is associated with tumor cell proliferation and poor prognosis PMID: 26059795
The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators. PMID: 26381409
NF45 and NF90 are novel higher-eukaryote-specific factors required for the maturation of 60S ribosomal subunits. PMID: 26240280
These findings suggested that NF45 might play an important role in promoting the tumorigenesis of ESCC, and thus be a promising therapeutic target to prevent ESCC progression. PMID: 25286760
These results suggest a novel molecular mechanism for the modulation of RNA granule assembly and disassembly by NFAR2, NF45, and phosphorylation at double-stranded RNA-activated kinase PKR sites. PMID: 24920670
These findings indicate that NF45 plays an important role in the growth regulation of glioma cells PMID: 25023405
AU-rich 5' UTRs harboring internal ribosome entry sites are regulated by NF45. NF45 regulates XIAP protein levels through interaction with its IRES. PMID: 23129811
The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining PMID: 21969602
ILF2 recruits a DNA-associated replication and transcription complex, of which it is a part, to its target DNA sequence. PMID: 20808282
The data presented are consistent with a model in which translation of cIAP1 is governed, at least in part, by NF45, a novel cellular IRES trans-acting factor. PMID: 19893574
plays important role in protein priming of HBV polymerase PMID: 11958450
NF45 is a is a highly conserved transcriptional activator of the gene IL-2. PMID: 15817156
Cell growth is retarded and giant multinucleated cells accumulate when the expression of NF45 or NF90, but not NF110, is reduced in HeLa cells. PMID: 18458058
Results suggest that the association of the NF90-NF45 complex with pri-miRNAs impairs access of the Microprocessor complex to the pri-miRNAs, resulting in a reduction of mature miRNA production. PMID: 19398578

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Subcellular Location

Nucleus, nucleolus. Cytoplasm. Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Database Links

HGNC: 6037

OMIM: 603181

KEGG: hsa:3608

STRING: 9606.ENSP00000355011

UniGene: Hs.75117

Code	CSB-EP614262HU
Abbreviation	Recombinant Human ILF2 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Interleukin enhancer-binding factor 2 (ILF2)

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