ILF2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA145432A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Raji whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: ILF2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time：20s IHC image of CSB-RA145432A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA145432A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA145432A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

Q12905

Target Names

ILF2

Alternative Names

HGNC:6037 antibody; ILF 2 antibody; ilf2 antibody; ILF2_HUMAN antibody; Interleukin enhancer binding factor 2 antibody; Interleukin enhancer binding factor 2; 45kDa antibody; Interleukin enhancer-binding factor 2 antibody; MGC8391 antibody; NF 45 antibody; NF45 antibody; Nuclear factor of activated T cells 45 kDa antibody; Nuclear factor of activated T-cells 45 kDa antibody; PRO3063 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human ILF2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8F2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Interleukin enhancer-binding factor 2, commonly known as NF45, plays a critical role in transcriptional regulation and RNA processing within the nucleus. As a component of the NF45-NF90 complex, ILF2 participates in gene expression control, DNA repair mechanisms, and cellular stress responses, making it a valuable target for researchers investigating epigenetics and nuclear signaling pathways.

This recombinant monoclonal antibody, clone 8F2, offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents, ensuring your results remain comparable across extended studies and collaborative projects.

Extensive validation demonstrates reliable performance across multiple applications. Western blot analysis detects a clean band at the predicted 43 kDa molecular weight across a diverse panel of human cell lines, including HeLa, Jurkat, Raji, MCF-7, HepG2, PC-3, and A431, confirming robust detection in both adherent and suspension cell models. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human brain and breast cancer specimens shows specific staining using standard citrate buffer antigen retrieval protocols on automated Leica Bond systems. Flow cytometry applications have been validated in SH-SY5Y neuroblastoma cells, demonstrating clear signal separation from isotype controls in fixed and permeabilized samples.

The unconjugated format provides flexibility for pairing with your preferred secondary detection systems. Whether you are exploring transcriptional regulation, characterizing nuclear protein complexes, or investigating ILF2 expression patterns in disease contexts, this antibody delivers the specificity and versatility needed for rigorous experimental design.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus.

Gene References into Functions

this study identifies a new role of ILF2 in the regulation of the NLRP3 inflammasome. PMID: 29655789
High expression of ILF2 was tightly correlated with lymph node metastasis, pathological stage and poor clinical prognosis in gastric cancer. PMID: 28831206
ILF2 interacts with RNA-binding proteins involved in the DNA damage response. PMID: 28669490
The data suggest that features of both the N- and C-termini of NF90 participate in the heterodimerization with NF45 and that the formation of NF90-NF45 changes the conformation of NF90's RNA-binding motifs to a status in which the co-operative interplay of the RNA-binding motifs is optimal. PMID: 28062840
NF90-NF45 stimulates an elevation of EGF receptor levels via the suppression of miR-7 biogenesis, resulting in the promotion of cell proliferation in Hepatocellular Carcinoma. PMID: 27519414
The results revealed that ILF2 is regulated by miR-7 and suggest that downregulation of miR-7 may be an important factor for the ILF2 overexpression in pancreatic carcinoma. PMID: 28259961
Deletion of the RNA binding domains of NF45 and NF90 diminished the enhancement of HIV infection and gene expression. PMID: 26891316
NF45 overexpression is associated with poor prognosis and enhanced cell proliferation of pancreatic ductal adenocarcinoma. PMID: 26276310
Upregulated expression of ILF2 in non-small cell lung cancer is associated with tumor cell proliferation and poor prognosis PMID: 26059795
The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators. PMID: 26381409
NF45 and NF90 are novel higher-eukaryote-specific factors required for the maturation of 60S ribosomal subunits. PMID: 26240280
These findings suggested that NF45 might play an important role in promoting the tumorigenesis of ESCC, and thus be a promising therapeutic target to prevent ESCC progression. PMID: 25286760
These results suggest a novel molecular mechanism for the modulation of RNA granule assembly and disassembly by NFAR2, NF45, and phosphorylation at double-stranded RNA-activated kinase PKR sites. PMID: 24920670
These findings indicate that NF45 plays an important role in the growth regulation of glioma cells PMID: 25023405
AU-rich 5' UTRs harboring internal ribosome entry sites are regulated by NF45. NF45 regulates XIAP protein levels through interaction with its IRES. PMID: 23129811
The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining PMID: 21969602
ILF2 recruits a DNA-associated replication and transcription complex, of which it is a part, to its target DNA sequence. PMID: 20808282
The data presented are consistent with a model in which translation of cIAP1 is governed, at least in part, by NF45, a novel cellular IRES trans-acting factor. PMID: 19893574
plays important role in protein priming of HBV polymerase PMID: 11958450
NF45 is a is a highly conserved transcriptional activator of the gene IL-2. PMID: 15817156
Cell growth is retarded and giant multinucleated cells accumulate when the expression of NF45 or NF90, but not NF110, is reduced in HeLa cells. PMID: 18458058
Results suggest that the association of the NF90-NF45 complex with pri-miRNAs impairs access of the Microprocessor complex to the pri-miRNAs, resulting in a reduction of mature miRNA production. PMID: 19398578

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Subcellular Location

Nucleus, nucleolus. Cytoplasm. Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Database Links

HGNC: 6037

OMIM: 603181

KEGG: hsa:3608

STRING: 9606.ENSP00000355011

UniGene: Hs.75117

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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