Recombinant Human Iron-sulfur cluster assembly 2 homolog, mitochondrial (ISCA2)

Code CSB-YP769773HU
MSDS
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Source Yeast
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Code CSB-EP769773HU
MSDS
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Source E.coli
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Code CSB-EP769773HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP769773HU
MSDS
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Source Baculovirus
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Code CSB-MP769773HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ISCA2
Uniprot No.
Alternative Names
c14_5557 ; HESB like domain containing 1; HESB like domain containing protein 1 ; HESB-like domain-containing protein 1; iron sulfur cluster assembly 2 homolog (S. cerevisiae); Iron sulfur cluster assembly 2 homolog; mitochondrial ; Iron-sulfur cluster assembly 2 homolog; mitochondrial; ISCA2; ISCA2_HUMAN
Species
Homo sapiens (Human)
Expression Region
9-154
Target Protein Sequence
LT AATQRAVTPW PRGRLLTASL GPQARREASS SSPEAGEGQI RLTDSCVQRL LEITEGSEFL RLQVEGGGCS GFQYKFSLDT VINPDDRVFE QGGARVVVDS DSLAFVKGAQ VDFSQELIRS SFQVLNNPQA QQGCSCGSSF SIKL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway. May be involved in the binding of an intermediate of Fe/S cluster assembly.
Gene References into Functions
  1. We identified a homoallelic missense founder mutation in ISCA2 leading to mitochondrial depletion and reduced complex I activity as well as decreased ISCA2, ISCA1 and IBA57 expression in fibroblasts. PMID: 25539947
Involvement in disease
Multiple mitochondrial dysfunctions syndrome 4 (MMDS4)
Subcellular Location
Mitochondrion.
Protein Families
HesB/IscA family
Database Links

HGNC: 19857

OMIM: 615317

KEGG: hsa:122961

STRING: 9606.ENSP00000452007

UniGene: Hs.291079

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