Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ISM1

Uniprot No.

B1AKI9

Research Area

others

Alternative Names

ISM1; C20orf82; ISM; Isthmin-1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

30-464aa

Target Protein Sequence

ADGPDAAAGNASQAQLQNNLNVGSDTTSETSFSLSKEAPREHLDHQAAHQPFPRPRFRQETGHPSLQRDFPRSFLLDLPNFPDLSKADINGQNPNIQVTIEVVDGPDSEADKDQHPENKPSWSVPSPDWRAWWQRSLSLARANSGDQDYKYDSTSDDSNFLNPPRGWDHTAPGHRTFETKDQPEYDSTDGEGDWSLWSVCSVTCGNGNQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEEFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVMNDLPSCPCSYPTEVAYSTADIFDRIKRKDFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDVLPWIICKGDWSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

69.1 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant Human ISM1 protein is a prokaryotic system E.coli expressed protein (Full Length of Mature Protein). To make this ISM1 recombinant protein, the ISM1 gene is synthesized and cloned into the E.coli system expression vector, and then it was transformed into protein-expressing host E Coli for expression, the other steps are testing for the identification of recombinant protein, large scale production and target protein Isolation and purification. The purity of the final ISM1 protein is 85%+ as determined by SDS-PAGE.

ISM1 (also known as ISM or C20f82) is a protein encoding gene that provides an instruction in making a protein named Isthmin-1. isthmin-1 is a 52-kDa secreted protein with a thrombospondin type 1 repeat (TSR) domain, containing two consensus C-mannosylation sequences at Trp223 and Trp226 in human. This protein has a dual role in increasing adipose glucose uptake while suppressing hepatic lipid synthesis as an adipokine. It is also essential during organ development and active in parts of the immune response.

Target Background

Function

Acts as an angiogenesis inhibitor.

Gene References into Functions

Our study demonstrates a successful pipeline from CNV identification of a candidate gene to functional validation in a vertebrate model system, and reveals Isthmin 1 as both a new human clefting locus as well as a key craniofacial patterning gene. PMID: 29162626
Given that ISM1 has been reported to have anti-angiogenic properties, these observations suggest that ISM1 is a mediator of lymphocyte effector functions and may participate in both innate and acquired immune responses. PMID: 24956034
Isthmin unexpectedly has both a pro-survival and death-promoting effect on endothelial cells depending on its physical state. PMID: 21544092

Subcellular Location

Secreted.

Protein Families

Isthmin family

Database Links

HGNC: 16213

OMIM: 615793

KEGG: hsa:140862

STRING: 9606.ENSP00000262487

UniGene: Hs.559353

Code	CSB-EP011850HU
Abbreviation	Recombinant Human ISM1 protein
MSDS	MSDS Datasheet
Size	$256
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Isthmin-1 (ISM1)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage