Recombinant Human Izumo sperm-egg fusion protein 1 (IZUMO1), partial

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Code CSB-EP812903HU
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
IZUMO1
Uniprot No.
Research Area
Developmental Biology
Alternative Names
FLJ61440; IZUM1_HUMAN; IZUMO; Izumo sperm egg fusion 1; Izumo sperm-egg fusion protein 1; Izumo1; MGC34799; OBF; Oocyte binding/fusion factor; Sperm specific protein izumo; Sperm-specific protein izumo
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
22-292aa
Target Protein Sequence
CVICDPSVVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
38.1 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The region for expressing recombinant Human IZUMO1 contains amino acids 22-292. This IZUMO1 protein is theoretically predicted to have a molecular weight of 38.1 kDa. This protein is generated in a e.coli-based system. The IZUMO1 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant IZUMO1 protein during the following stages.

The human Izumo sperm-egg fusion protein 1 (IZUMO1) is a transmembrane protein found on the surface of sperm cells. It plays a crucial role in the process of fertilization by mediating the fusion between sperm and egg during gamete interaction. IZUMO1 is essential for establishing the initial contact between the sperm and the egg, leading to the formation of a fertilization pore and subsequent fusion of the two gametes' membranes. The molecular interactions involving IZUMO1 are part of the intricate and highly regulated mechanisms that govern the fusion event, ensuring successful fertilization. Understanding IZUMO1's function provides valuable insights into reproductive biology and potential avenues for contraceptive development.

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Target Background

Function
Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.
Gene References into Functions
  1. crystal structures of human IZUMO1, JUNO and the IZUMO1-JUNO complex, establishing the structural basis for the IZUMO1-JUNO-mediated sperm-oocyte interaction PMID: 27309808
  2. crystal structures of human IZUMO1 and JUNO in unbound and bound conformations PMID: 27309818
  3. these findings indicate that human sperm expresses Izumo protein, and the immunoinfertile female sera have circulating isoantibodies against this protein. PMID: 23279005
  4. human sperm contain Izumo and addition of the antibody against human Izumo left the sperm unable to fuse with zona-free hamster eggs [Izumo] PMID: 15759005
  5. We observed in our populations two combinations of four polymorphisms that appeared to be preferentially linked (CGG-CG and TAA-TT) without any significant difference between different genotype repartitions. PMID: 18082733
  6. Observational study of gene-disease association. (HuGE Navigator) PMID: 18082733

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein.
Protein Families
Izumo family
Tissue Specificity
Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.
Database Links

HGNC: 28539

OMIM: 609278

KEGG: hsa:284359

STRING: 9606.ENSP00000327786

UniGene: Hs.400688

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