Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

IZUMO1

Uniprot No.

Q8IYV9

Research Area

Developmental Biology

Alternative Names

FLJ61440; IZUM1_HUMAN; IZUMO; Izumo sperm egg fusion 1; Izumo sperm-egg fusion protein 1; Izumo1; MGC34799; OBF; Oocyte binding/fusion factor; Sperm specific protein izumo; Sperm-specific protein izumo

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

22-292aa

Target Protein Sequence

CVICDPSVVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

38.1 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human IZUMO1 contains amino acids 22-292. This IZUMO1 protein is theoretically predicted to have a molecular weight of 38.1 kDa. This protein is generated in a e.coli-based system. The IZUMO1 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant IZUMO1 protein during the following stages.

The human Izumo sperm-egg fusion protein 1 (IZUMO1) is a transmembrane protein found on the surface of sperm cells. It plays a crucial role in the process of fertilization by mediating the fusion between sperm and egg during gamete interaction. IZUMO1 is essential for establishing the initial contact between the sperm and the egg, leading to the formation of a fertilization pore and subsequent fusion of the two gametes' membranes. The molecular interactions involving IZUMO1 are part of the intricate and highly regulated mechanisms that govern the fusion event, ensuring successful fertilization. Understanding IZUMO1's function provides valuable insights into reproductive biology and potential avenues for contraceptive development.

Target Background

Function

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.

Gene References into Functions

crystal structures of human IZUMO1, JUNO and the IZUMO1-JUNO complex, establishing the structural basis for the IZUMO1-JUNO-mediated sperm-oocyte interaction PMID: 27309808
crystal structures of human IZUMO1 and JUNO in unbound and bound conformations PMID: 27309818
these findings indicate that human sperm expresses Izumo protein, and the immunoinfertile female sera have circulating isoantibodies against this protein. PMID: 23279005
human sperm contain Izumo and addition of the antibody against human Izumo left the sperm unable to fuse with zona-free hamster eggs [Izumo] PMID: 15759005
We observed in our populations two combinations of four polymorphisms that appeared to be preferentially linked (CGG-CG and TAA-TT) without any significant difference between different genotype repartitions. PMID: 18082733
Observational study of gene-disease association. (HuGE Navigator) PMID: 18082733

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein.

Protein Families

Izumo family

Tissue Specificity

Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.

Database Links

HGNC: 28539

OMIM: 609278

KEGG: hsa:284359

STRING: 9606.ENSP00000327786

UniGene: Hs.400688

Code	CSB-EP812903HU
Abbreviation	Recombinant Human IZUMO1 protein, partial
MSDS	MSDS Datasheet
Size	$306
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Recombinant Human Izumo sperm-egg fusion protein 1 (IZUMO1), partial

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