Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Developmental Biology
Alternative Names
FLJ61440; IZUM1_HUMAN; IZUMO; Izumo sperm egg fusion 1; Izumo sperm-egg fusion protein 1; Izumo1; MGC34799; OBF; Oocyte binding/fusion factor; Sperm specific protein izumo; Sperm-specific protein izumo
Species
Homo sapiens (Human)
Expression Region
22-292aa
Target Protein Sequence
CVICDPSVVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASR
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Human IZUMO1 contains amino acids 22-292. This IZUMO1 protein is theoretically predicted to have a molecular weight of 38.1 kDa. This protein is generated in a e.coli-based system. The IZUMO1 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant IZUMO1 protein during the following stages.
The human Izumo sperm-egg fusion protein 1 (IZUMO1) is a transmembrane protein found on the surface of sperm cells. It plays a crucial role in the process of fertilization by mediating the fusion between sperm and egg during gamete interaction. IZUMO1 is essential for establishing the initial contact between the sperm and the egg, leading to the formation of a fertilization pore and subsequent fusion of the two gametes' membranes. The molecular interactions involving IZUMO1 are part of the intricate and highly regulated mechanisms that govern the fusion event, ensuring successful fertilization. Understanding IZUMO1's function provides valuable insights into reproductive biology and potential avenues for contraceptive development.
