Recombinant Human Kelch domain-containing protein 5 (KLHDC5)

Code CSB-YP885803HU
MSDS
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Source Yeast
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Code CSB-EP885803HU
MSDS
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Source E.coli
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Code CSB-EP885803HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885803HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP885803HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KLHL42
Uniprot No.
Alternative Names
KLHL42; KIAA1340; KLHDC5Kelch-like protein 42; Cullin-3-binding protein 9; Ctb9; Kelch domain-containing protein 5
Species
Homo sapiens (Human)
Expression Region
1-505
Target Protein Sequence
MSAEEMVQIR LEDRCYPVSK RKLIEQSDYF RALYRSGMRE ALSQEAGGPE VQQLRGLSAP GLRLVLDFIN AGGAREGWLL GPRGEKGGGV DEDEEMDEVS LLSELVEAAS FLQVTSLLQL LLSQVRLNNC LEMYRLAQVY GLPDLQEACL RFMVVHFHEV LCKPQFHLLG SPPQAPGDVS LKQRLREARM TGTPVLVALG DFLGGPLAPH PYQGEPPSML RYEEMTERWF PLANNLPPDL VNVRGYGSAI LDNYLFIVGG YRITSQEISA AHSYNPSTNE WLQVASMNQK RSNFKLVAVN SKLYAIGGQA VSNVECYNPE QDAWNFVAPL PNPLAEFSAC ECKGKIYVIG GYTTRDRNMN ILQYCPSSDM WTLFETCDVH IRKQQMVSVE ETIYIVGGCL HELGPNRRSS QSEDMLTVQS YNTVTRQWLY LKENTSKSGL NLTCALHNDG IYIMSRDVTL STSLEHRVFL KYNIFSDSWE AFRRFPAFGH NLLVSSLYLP NKAET
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL42) E3 ubiquitin ligase complex mediates the ubiquitination and subsequent degradation of KATNA1. Involved in microtubule dynamics throughout mitosis.
Gene References into Functions
  1. Cul3-deficient cells or Ctb9/KLHDC5-deficient cells show an increase in p60/katanin levels, indicating that Cul3/Ctb9/KLHDC5 is required for efficient p60/katanin removal PMID: 19261606
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, spindle. Note=Predominantly in mitotic cells. Localized diffusely in the cytoplasm during the interphase. During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase cells is localized between the two sets of separated chromosomes as well as at the spindle poles. During telophase is localized arround the nuclei of the two daughter cells. Not detected at the midbody region during cytokinesis.
Database Links

HGNC: 29252

KEGG: hsa:57542

STRING: 9606.ENSP00000370671

UniGene: Hs.505104

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