Recombinant Human Kv channel-interacting protein 4 (KCNIP4)

Code CSB-YP750882HU
MSDS
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Source Yeast
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Code CSB-EP750882HU
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Source E.coli
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Code CSB-EP750882HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP750882HU
MSDS
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Source Baculovirus
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Code CSB-MP750882HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KCNIP4
Uniprot No.
Alternative Names
A type potassium channel modulatory protein 4; A-type potassium channel modulatory protein 4; CALP; Calsenilin like protein; Calsenilin-like protein; HGNC:30083; KCHIP 4; KChIP4; KCIP4_HUMAN; KCNIP 4; Kcnip4; Kv channel interacting protein 4; Kv channel-interacting protein 4; MGC44947; Potassium channel interacting protein 4; Potassium channel-interacting protein 4
Species
Homo sapiens (Human)
Expression Region
1-250
Target Protein Sequence
MNVRRVESIS AQLEEASSTG GFLYAQNSTK RSIKERLMKL LPCSAAKTSS PAIQNSVEDE LEMATVRHRP EALELLEAQS KFTKKELQIL YRGFKNECPS GVVNEETFKE IYSQFFPQGD STTYAHFLFN AFDTDHNGAV SFEDFIKGLS ILLRGTVQEK LNWAFNLYDI NKDGYITKEE MLDIMKAIYD MMGKCTYPVL KEDAPRQHVE TFFQKMDKNK DGVVTIDEFI ESCQKDENIM RSMQLFENVI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates KCND2 channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. Modulates KCND3/Kv4.3 currents. Isoform 4 does not increase KCND2 expression at the cell membrane. Isoform 4 retains KCND3 in the endoplasmic reticulum and negatively regulates its expression at the cell membrane.
Gene References into Functions
  1. Results indicate that SNPs in KCNIP4 may modulate ACE inhibitor-induced cough risk. PMID: 26169577
  2. Subunit counting by single-molecule imaging revealed that the bound number of KChIP4 in each Kv4.2.KChIP4 complex was dependent on the expression level of KChIP4. PMID: 24811166
  3. genetic association studies in populations in Germany: Data suggest that multiple SNPs in KCNIP4 are associated not only with attention deficit disorder with hyperactivity in children and adults but also with personality disorders. PMID: 22981920
  4. Genome-wide association study identifies KCNIP4 as an asthma gene in human and in mouse. PMID: 23457522
  5. KChIP4a suppresses A-type Kv4 current via ER retention and enhancement of Kv4 closed-state inactivation. PMID: 23576435
  6. The synthesis of the variant KCNIP4 isoform is also detrimental to brain physiology, as it results in the concomitant blockade of the fast kinetics of potassium channels. PMID: 21624954
  7. These data demonstrate that PKA phosphorylation of Kv4.2 plays an important role in the trafficking of Kv4.2 through its specific interaction with KChIP4a. PMID: 20045463
  8. cloning and investigation of interaction with presenilin 2 and voltage-gated potassium channel subunit Kv4 PMID: 11847232
  9. The conformational change with metal-bound KChIP4.1 is crucial for its interaction with Kv channel but not for KChIP2.2, and that the Mg2+- and Ca2+-binding properties of KChIP2.2 and KChIP4.1 have different effects on their molecular structure. PMID: 16951992
  10. Mutation of KCNIP4 is associated with renal cell carcinoma. PMID: 17981209
  11. CALP and calsenilin are involved in the trafficking of Golgi glycosyltransferases. PMID: 18269347
  12. These results reveal a new role for KChIP3 in the regulation of calcium regulated secretion and also suggest that the functions of each of the KChIPs may be more specialized than previously appreciated. PMID: 18393943
  13. KChIP4a, KChIP2x, and KChIP3x comprise a novel class of KChIP isoforms characterized by an unusual transmembrane domain at their N termini that modulates Kv4 channel gating and trafficking. PMID: 18957440

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Subcellular Location
Cell membrane; Peripheral membrane protein. Cytoplasm.; [Isoform 4]: Endoplasmic reticulum.
Protein Families
Recoverin family
Tissue Specificity
Predominantly expressed in brain.
Database Links

HGNC: 30083

OMIM: 608182

KEGG: hsa:80333

STRING: 9606.ENSP00000371587

UniGene: Hs.655705

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