Recombinant Human Glutathione S-transferase LANCL1 (LANCL1)

Inserting the gene encoding the Human LANCL1 protein (2-399aa) into a plasmid vector results in the creation of recombinant plasmid, which is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected, indicating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human LANCL1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human LANCL1 protein, revealing a purity level exceeding 85%.