Recombinant Human Leucine-rich repeat-containing protein 16A (LRRC16A), partial

Code CSB-YP713356HU
MSDS
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Source Yeast
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Code CSB-EP713356HU
MSDS
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Source E.coli
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Code CSB-EP713356HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP713356HU
MSDS
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Source Baculovirus
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Code CSB-MP713356HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CARMIL1
Uniprot No.
Alternative Names
Acan 125; Capping protein; Arp2/3; and myosin I linker; CARMIL homolog; leucine rich repeat containing 16; Leucine-rich repeat-containing protein 16A; LR16A_HUMAN; Lrrc16 protein; LRRC16A; p116
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization, however, seems unable to nucleate filaments. Plays a role in lamellipodial protrusion formations and cell migration.
Gene References into Functions
  1. A novel genetic variant in leucine-rich repeat-containing 16A (LRRC16A) has been associated with reduced ARDS risk. PMID: 27768389
  2. LRRC16A plays a role in adult respiratory distress syndrome pathophysiology by interacting with, and being mediated through, platelets. PMID: 25254322
  3. shown for the first time that CARMIL/LRRC16A was associated with gout, which could be due to urate transportsome failure PMID: 24318514
  4. The results also suggest that the ability of CARMIL1 to inhibit CP in cells may be regulated. PMID: 23904264
  5. Data suggest that CARMIL promotes uncapping by binding to a freely accessible site on Capping protein (CP) bound to a filament barbed end and inducing a change in the conformation of the actin-binding surface of CP. PMID: 22411988
  6. The two CARMIL isoforms are both important for cell migration, but they have distinct functions.[CARMIL1, CARMIL2] PMID: 19846667

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium.
Protein Families
CARMIL family
Tissue Specificity
Expressed in lung, placenta, small intestine, liver, thymus, colon, skeletal muscle, heart and brain. Higher expression in kidney.
Database Links

HGNC: 21581

OMIM: 609593

KEGG: hsa:55604

STRING: 9606.ENSP00000331983

UniGene: Hs.649550

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