Recombinant Human Lysophospholipase-like protein 1 (LYPLAL1)

Code CSB-YP736204HU
MSDS
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Source Yeast
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Code CSB-EP736204HU
MSDS
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Source E.coli
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Code CSB-EP736204HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP736204HU
MSDS
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Source Baculovirus
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Code CSB-MP736204HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LYPLAL1
Uniprot No.
Alternative Names
BC027340; FLJ99730; KIAA1238; LYPL1_HUMAN; LYPLAL1; Lysophospholipase like 1; Lysophospholipase-like protein 1; MGC28394; OTTHUMP00000035229
Species
Homo sapiens (Human)
Expression Region
2-237
Target Protein Sequence
AAASGSVLQ RCIVSPAGRH SASLIFLHGS GDSGQGLRMW IKQVLNQDLT FQHIKIIYPT APPRSYTPMK GGISNVWFDR FKITNDCPEH LESIDVMCQV LTDLIDEEVK SGIKKNRILI GGFSMGGCMA IHLAYRNHQD VAGVFALSSF LNKASAVYQA LQKSNGVLPE LFQCHGTADE LVLHSWAEET NSMLKSLGVT TKFHSFPNVY HELSKTELDI LKLWILTKLP GEMEKQK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has depalmitoylating activity toward KCNMA1. Does not exhibit phospholipase nor triacylglycerol lipase activity, able to hydrolyze only short chain substrates due to its shallow active site.
Gene References into Functions
  1. Novel LYPLAL1 SNP rs2605100 is associated with childhood hypertension adjusted by obesity. PMID: 28645872
  2. The LYPLAL1 genotype is associated with differences in eating behavior and loss of extensive body weight following Roux-en-Y gastric bypass (RYGB) surgery. Genotyping and the use of eating behavior-related questionnaires may help to estimate the RYGB-associated therapy success. PMID: 27181159
  3. Data provide evidence that variants of MC4R and LYPLAL1 modulate body fat distribution with sexual dimorphism in a Chinese population. PMID: 26848030
  4. Our results suggest that LYPLAL1 rs4846567 and NISCH rs6784615 may influence fat distribution in the Japanese population. PMID: 23221025
  5. genetic association studies in Danish population: Quantitative metabolic phenotypes in obesity are associated with SNP in various genes: LYPLAL1 (rs4846567) is associated with waist-hip ratio and insulin sensitivity in women. PMID: 21953277
  6. Gene-treatment interactions were observed for short-term weight loss. (LYPLAL1 rs2605100, Plifestyle*SNP = 0.032) PMID: 22179955
  7. LYPLAL1 exhibits neither phospholipase nor triacylglycerol lipase activity, but rather accepts short-chain substrates PMID: 22052940
  8. central obesity-associated variants in LYPLAL1, NRXN3, MSRA, and TFAP2B PMID: 21674055
  9. TFAP2B, LYPLAL1 and MSRA are associated with adiposity and fat distribution. PMID: 19557161

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Subcellular Location
[Isoform 1]: Cytoplasm, cytosol.; [Isoform 2]: Cytoplasm, cytosol.
Protein Families
AB hydrolase superfamily, AB hydrolase 2 family
Database Links

HGNC: 20440

OMIM: 616548

KEGG: hsa:127018

STRING: 9606.ENSP00000355895

UniGene: Hs.657617

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